胃癌患者血清中miR-133a水平及其与病理特征的相关性

发布时间：2019-05-21 08:04

【摘要】：目的:探讨血清中miR-133a水平对胃癌的诊断价值及其与病理特征的相关性。方法:取2010年3月至2011年4月于南京医科大学附属常州市第二附属医院胃肠外科住院的胃癌手术患者94例(胃癌组),其中男64例、女30例,年龄32~82岁,平均(61.72±8.59)岁;取30例健康体检者作为对照组。实时荧光定量PCR法检测两组血清中miR-133a水平,应用SPSS 22.0和Graphpad 5.0统计软件进行统计学分析。结果:胃癌患者血清中miR-133a水平明显低于正常对照组(1.95±1.51 vs 4.47±0.56,P0.01)。患者miR-133a水平与其年龄、性别、肿瘤大小无明显相关性(P0.05),与肿瘤分化程度、浸润深度、淋巴结转移及TNM分期有关(均P0.05)。受试者工作特征曲线(ROC)分析结果显示,miR-133a在预测胃癌发生和淋巴结转移时具有较好的敏感性和特异性[ROC曲线下面积(AUC)分别为0.883(95%CI:0.8257~0.9402)和0.984(95%CI:0.9633~1.005),均P0.01]。miR-133a高表达组患者5年生存率显著高于miR-133a低表达组(40.49%vs 19.37%,P0.05)。结论:胃癌患者血清miR-133a表达水平改变与胃癌的发生发展、临床病理特征及临床预后密切相关,miR-133a可能成为胃癌诊断及预后判断的液体活检标志物。
[Abstract]:Objective: to investigate the diagnostic value of serum miR-133a level in gastric cancer and its correlation with pathological features. Methods: from March 2010 to April 2011, 94 patients with gastric cancer, including 64 males and 30 females, aged 32 years and 82 years, were enrolled in the Department of Gastrointestinal surgery, the second affiliated Hospital of Changzhou City, Nanjing Medical University. The average age was (61.72 卤8.59) years. Thirty healthy subjects were taken as the control group. The levels of miR-133a in serum of the two groups were detected by real-time fluorescence quantitative PCR and analyzed statistically by SPSS 22.0 and Graphpad 5.0 statistical software. Results: the level of miR-133a in serum of patients with gastric cancer was significantly lower than that of normal control group (1.95 卤1.51 vs 4.47 卤0.56, P 0.01). There was no significant correlation between miR-133a level and age, sex and tumor size (P 0.05), but correlated with tumor differentiation, depth of invasion, lymph node metastasis and TNM stage (P 0.05). The results of (ROC) analysis of the working characteristic curve of the subjects showed that MiR-133a has good sensitivity and specificity in predicting the occurrence of gastric cancer and lymph node metastasis [the area (AUC) under ROC curve is 0.883 (95%CI:0.8257~0.9402) and 0.984 (95%CI:0.9633~1.005), respectively. The 5-year survival rate of patients with high expression of miR-133a was significantly higher than that of patients with low expression of miR-133a (40.49%vs 19.37%, P 0.05). Conclusion: the change of serum miR-133a expression level in patients with gastric cancer is closely related to the occurrence and development of gastric cancer, clinicopathological features and clinical prognosis. MiR-133a may become a liquid biopsies marker for the diagnosis and prognosis of gastric cancer.
【作者单位】：南京医科大学附属常州市第二人民医院胃肠外科;
【基金】：常州市科技基础研究项目资助(No.CJ20122014)~~
【分类号】：R735.2

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