微波消融对原发性肝癌患者免疫功能的影响
发布时间：2019-06-06 07:50
【摘要】：目的本课题主要分析原发性肝癌患者在经皮微波消融治疗后机体部分免疫指标的变化及其意义,研究经皮微波消融治疗对肝癌患者机体免疫功能造成的影响,为临床选择合适的治疗手段及判断预后提供客观依据。方法统计了2014年2月至2016年2月两年期间,在解放军第四0一医院肿瘤科所进行的经皮微波消融治疗的48例原发性肝癌患者,分别在手术之前的3天、手术之后第8天以及手术后第30天抽取外周静脉血3毫升,分别检测T淋巴细胞亚群、自然杀伤细胞(NK细胞)、免疫细胞亚群因子(IFN-γ、TNF-α、IL-12、IL-2、IL-10、IL-4)和炎症细胞因子(IL-1β、IL-6、IL-8、IP-10、MCP-1/MCAF、IL-17)水平,进行前后对照,同时比较术前3天、术后30天外周血AFP的变化情况。结果1、经皮微波消融治疗后第8天患者外周血NK细胞比例相比手术前3天明显升高,IL-8比例较治疗前显著降低,差异有统计学意义(P值分别为0.004和0.028)。2、治疗后第30天外周血NK细胞、IL-12、IFN-γ、TNF-α、CD4+T/CD8+T比值水平较治疗前显著升高,差异有统计学意义(P值分别为0.0004、0.028、0.043、0.018、0.035),而IL-4、IL-6、调节性T细胞所占比例较治疗前显著降低,差异有统计学意义(P值分别为0.019、0.008、0.0003)。3、26例患者治疗前外周血AFP高于正常者,治疗后第30天均有不同程度降低,治疗前后外周血AFP差异有统计学意义(t=2.39,P0.05)。4、所有免疫细胞亚群及细胞因子治疗后第8天与治疗后第30天比较,差异均无统计学意义(均P0.05)。结论1、原发性肝癌患者经皮微波消融治疗后外周血NK细胞、IL-12、IFN-γ、TNF-α、CD4+T/C D8+T水平较治疗前明显提高,调节性T细胞、IL-4较治疗前显著降低提示经皮微波消融治疗可直接或间接刺激机体产生抗肿瘤效应,增强肝癌患者的细胞免疫功能。2、IL-8、IL-6、AFP治疗后显著降低,提示经皮微波消融治疗可以有效降低原发性肝癌患者肿瘤负荷且具有较好的近期疗效。
[Abstract]:Objective to analyze the changes and significance of some immune indexes in patients with primary liver cancer after percutaneous microwave ablation, and to study the effect of percutaneous microwave ablation on immune function in patients with primary liver cancer. It provides an objective basis for clinical selection of appropriate treatment and prognosis. Methods from February 2014 to February 2016, 48 patients with primary liver cancer were treated by percutaneous microwave ablation in the Department of Oncology, 40th Hospital of the people's Liberation Army, respectively, three days before the operation. On the 8th day after operation and on the 30th day after operation, 3 milliliters of peripheral venous blood were taken to detect T lymphocyte subsets, natural killer cells (NK cells), immune cell subgroup factors (IFN- 纬, TNF- 伪, IL-12,IL-2,). The levels of IL-10,IL-4 and IL-1 尾 (IL-6,IL-8,IP-10,MCP-1/MCAF,IL-17) were compared before and after operation, and the changes of AFP in peripheral blood 3 days before and 30 days after operation were compared. Results 1. The proportion of NK cells in peripheral blood of patients on the 8th day after percutaneous microwave ablation was significantly higher than that on the 3rd day before operation, and the proportion of IL-8 was significantly lower than that before treatment (P = 0.004 and 0.028, respectively). On the 30th day after treatment, the ratios of IL-12,IFN- 纬, TNF- 伪 and CD4 T/CD8 T in peripheral blood NK cells were significantly higher than those before treatment (P = 0.0004, 0.028, 0.043, 0.018, 0.035, respectively), while IL-4, was significantly higher than that before treatment (P = 0.0004, 0.028, 0.043, 0.018, 0.035, respectively). The proportion of IL-6, regulatory T cells was significantly lower than that before treatment (P = 0.019, 0.008, 0.0003, respectively). 3, AFP in peripheral blood of 26 patients before treatment was higher than that of normal patients. On the 30th day after treatment, the level of AFP in peripheral blood was significantly lower than that before and after treatment (t 鈮，
本文编号：2494188
[Abstract]:Objective to analyze the changes and significance of some immune indexes in patients with primary liver cancer after percutaneous microwave ablation, and to study the effect of percutaneous microwave ablation on immune function in patients with primary liver cancer. It provides an objective basis for clinical selection of appropriate treatment and prognosis. Methods from February 2014 to February 2016, 48 patients with primary liver cancer were treated by percutaneous microwave ablation in the Department of Oncology, 40th Hospital of the people's Liberation Army, respectively, three days before the operation. On the 8th day after operation and on the 30th day after operation, 3 milliliters of peripheral venous blood were taken to detect T lymphocyte subsets, natural killer cells (NK cells), immune cell subgroup factors (IFN- 纬, TNF- 伪, IL-12,IL-2,). The levels of IL-10,IL-4 and IL-1 尾 (IL-6,IL-8,IP-10,MCP-1/MCAF,IL-17) were compared before and after operation, and the changes of AFP in peripheral blood 3 days before and 30 days after operation were compared. Results 1. The proportion of NK cells in peripheral blood of patients on the 8th day after percutaneous microwave ablation was significantly higher than that on the 3rd day before operation, and the proportion of IL-8 was significantly lower than that before treatment (P = 0.004 and 0.028, respectively). On the 30th day after treatment, the ratios of IL-12,IFN- 纬, TNF- 伪 and CD4 T/CD8 T in peripheral blood NK cells were significantly higher than those before treatment (P = 0.0004, 0.028, 0.043, 0.018, 0.035, respectively), while IL-4, was significantly higher than that before treatment (P = 0.0004, 0.028, 0.043, 0.018, 0.035, respectively). The proportion of IL-6, regulatory T cells was significantly lower than that before treatment (P = 0.019, 0.008, 0.0003, respectively). 3, AFP in peripheral blood of 26 patients before treatment was higher than that of normal patients. On the 30th day after treatment, the level of AFP in peripheral blood was significantly lower than that before and after treatment (t 鈮，
本文编号：2494188
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