罗勒多糖抑制TGF-β诱导的A549细胞侵袭运动能力研究

发布时间：2019-06-07 15:03

【摘要】：目的罗勒多糖具有抗肿瘤侵袭转移、化疗增效以及降血糖、降血脂和抗血栓等多种生物活性作用。本研究探讨罗勒多糖对转化生长因子-β(transforming growth factor-beta,TGF-β)诱导的人肺癌A549细胞侵袭运动能力的抑制作用,揭示罗勒多糖抑制上皮间质转化作用的可能机制。方法体外培养肺癌A549细胞,实验分为3组:(1)正常对照组,不加任何处理;(2)模型组,TGF-β5ng/mL;(3)罗勒多糖组,TGF-β5ng/mL+罗勒多糖200μg/mL。免疫荧光检测罗勒多糖对E-cadherin、Vimentin和α-SMA表达的影响,细胞侵袭实验和运动实验检测细胞对人工基底膜的降解和运动能力,细胞划痕实验检测细胞运动能力。结果与正常对照组比较,模型组细胞Vimentin和α-SMA表达增强,E-cadherin表达减弱。罗勒多糖处理后Vimentin和α-SMA表达减弱,E-cadherin表达增强。TGF-β可提高细胞降解人工基底膜的能力,单位视野下侵袭至下室的细胞数模型组为69.0±4.3,与正常对照组(42.0±4.30)和罗勒多糖组(37.0±4.41)比较,差异有统计学意义,均P0.05。TGF-β增强了细胞的侵袭能力,罗勒多糖可以抑制TGF-β诱导的A549细胞侵袭能力。TGF-β使FN趋化的迁移细胞数目增多,单位视野下侵袭至下室的细胞数模型组为95.2±3.83,与正常对照组(69.2±5.11)和罗勒多糖组(68.2±6.18)比较,差异均有统计学意义,均P0.05。划痕实验显示,TGF-β增强了细胞的迁移能力,罗勒多糖可以抑制TGF-β诱导的A549细胞迁移能力。结论罗勒多糖能够调节A549细胞上皮间质转化,这可能是其抑制A549细胞的侵袭转移能力的重要机制。
[Abstract]:Objective basil polysaccharide has many biological activities, such as anti-tumor invasion and metastasis, chemotherapy synergy, hypoglycemia, blood lipid reduction and antithrombosis. The aim of this study was to investigate the inhibitory effect of basil polysaccharide on the invasiveness and motility of human lung cancer cell line A549 induced by transforming growth factor-尾 (transforming growth factor-beta,TGF- 尾 (transforming growth factor-beta,TGF- 尾), and to reveal the possible mechanism of basil polysaccharide inhibiting epithelial stroma transformation. Methods Lung cancer A549 cells were cultured in vitro and divided into three groups: (1) normal control group without any treatment; (2) model group, TGF- 尾 5 ng/mL; (3) basil polysaccharide group, TGF- 尾 5 ng/mL basil polysaccharide 200 渭 g / mL.. The effects of basil polysaccharide on the expression of E 鈮，

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