FOXF2在肝癌中的表达及其对预后价值的研究
发布时间：2019-06-14 12:23
【摘要】：原发性肝癌是世界上最常见的恶性肿瘤之一,同时在恶性肿瘤中,原发性肝癌致死率排名第三。据统计,在2008年,每年大概有700000人被诊断为原发性肝癌,其发病率为每100,000人中就有16人患有原发性肝癌。对于早期患者,我们可以选择肝脏肿瘤切除,肝移植及射频消融等手段进行治疗。然而高复发率是影响早期肝癌患者生存的主要障碍,在临床上,我们常用一些临床指标来预测患者治疗后的效果,其中包括肿瘤大小,血管侵润,AFP及病理分级等。然而,许多分子标志物来预测肝癌的预后还没有被公认,因此,积极寻找有效的分子标志物对于研究原发性的预后有着重要的意义。FOX家族是一个具有高保真的DNA区域和组织特异性表达的特点,其在调控细胞的生长分化,胚胎的形成及发育有着重要的作用。FOXF2作为FOX家族中的一员,已经被证实在乳腺癌及前列腺癌的发生发展中有着重要的作用。然而,其在肝癌中的表达及预后关系尚未有相关文献报道。[目的]本课题以肝癌为研究对象,选择FOXF2分子,检测在肝癌中的表达水平及与预后的关系,并在肝癌细胞系研究其在细胞水平上的表达与细胞的生物学行为的关系。[方法](1)利用免疫组化方法和real-time PCR检测295例肝癌及配对癌旁组织FOXF2表达并与临床病理学参数进行相关性分析。(2)利用IHC、Western Blot和real-time PCR方法检测295例肝癌组织和细胞的FOXF2表达并与临床病理学参数进行相关性分析。(3)通过人工合成FOXF2的干扰片,干涉MHCC-97H肝癌细胞系FOXF2的表达,检测其对肝癌细胞增殖、凋亡的影响。[结果]1.(1)通过IHC、Western Blot和real-time PCR技术检测结果,我们发现FOXF2在肝癌组织中的表达水平比癌旁低。2.(1) FOXF2在肝癌组织表达下降,另外在转移能力和侵袭能力较强的肝癌细胞系MHCC-97H中表达下降；(2)经统计分析结果显示FOXF2的低表达与肝癌患者的较差的预后呈正相关性；(3)关于FOXF2在肝癌细胞系中表达的初步功能研究发现FOXF2表达促进细胞的凋亡和抑制肝癌细胞的增殖；[结论]1.FOXF2表达下降在肝癌的发生发展中可能起重要作用,FOXF2的表达可作为肝癌患者术后生存的独立预测指标。2. FOXF2表达下降可提高肝癌细胞的抗凋亡能力并促进肝癌细胞的增殖。
[Abstract]:Primary liver cancer is one of the most common malignant tumors in the world, and the mortality rate of primary liver cancer ranks third among malignant tumors. According to statistics, in 2008, about 700000 people are diagnosed with primary liver cancer every year, and the incidence rate is 16 out of every 100000 people with primary liver cancer. For early patients, we can choose liver tumor resection, liver transplantation and radiofrequency ablation. However, high recurrence rate is the main obstacle to the survival of patients with early liver cancer. Clinically, we often use some clinical indicators to predict the effect of patients after treatment, including tumor size, vascular invasion, AFP and pathological grade. However, many molecular markers to predict the prognosis of liver cancer have not been recognized, so it is of great significance to actively find effective molecular markers for the study of primary prognosis. Fox family is a characteristic of high fidelity DNA region and tissue specific expression, which plays an important role in regulating cell growth and differentiation, embryo formation and development. FOXF2, as a member of FOX family, It has been proved to play an important role in the occurrence and development of breast cancer and prostate cancer. However, the relationship between its expression and prognosis in HCC has not been reported in the literature. [objective] in this study, FOXF2 molecule was selected to detect the expression level of HCC and its relationship with prognosis, and the relationship between the expression of HCC molecule and the biological behavior of HCC cell line was studied. [methods] (1) Immunohistochemical method and real-time PCR were used to detect the expression of FOXF2 in 295 cases of HCC and matched paracancerous tissues and their correlation with clinicopathological parameters. (2) the expression of FOXF2 in 295 cases of HCC tissues and cells was detected by IHC,Western Blot and real-time PCR, and the correlation between FOXF2 expression and clinicopathological parameters was analyzed. (3) the expression of FOXF2 in MHCC-97H HCC cell line was interfered with by synthetic FOXF2 interference. To detect the effect of HCC on proliferation and apoptosis of HCC cells. [results] 1. (1) the expression level of FOXF2 in HCC tissues was lower than that in HCC tissues by IHC,Western Blot and real-time PCR. (1) the expression of FOXF2 in HCC tissues decreased, in addition, in HCC cell line MHCC-97H with strong metastasis and invasiveness. (2) the results of statistical analysis showed that the low expression of FOXF2 was positively correlated with the poor prognosis of HCC patients. (3) the preliminary study on the expression of FOXF2 in HCC cell line found that the expression of FOXF2 promoted the apoptosis of HCC cells and inhibited the proliferation of HCC cells. [conclusion] the decrease of 1.FOXF2 expression may play an important role in the occurrence and development of HCC. The expression of FOXF2 can be used as an independent predictor of postoperative survival in patients with HCC. 2. The decrease of FOXF2 expression can improve the anti-apoptosis ability of HCC cells and promote the proliferation of HCC cells.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.7
本文编号：2499383
[Abstract]:Primary liver cancer is one of the most common malignant tumors in the world, and the mortality rate of primary liver cancer ranks third among malignant tumors. According to statistics, in 2008, about 700000 people are diagnosed with primary liver cancer every year, and the incidence rate is 16 out of every 100000 people with primary liver cancer. For early patients, we can choose liver tumor resection, liver transplantation and radiofrequency ablation. However, high recurrence rate is the main obstacle to the survival of patients with early liver cancer. Clinically, we often use some clinical indicators to predict the effect of patients after treatment, including tumor size, vascular invasion, AFP and pathological grade. However, many molecular markers to predict the prognosis of liver cancer have not been recognized, so it is of great significance to actively find effective molecular markers for the study of primary prognosis. Fox family is a characteristic of high fidelity DNA region and tissue specific expression, which plays an important role in regulating cell growth and differentiation, embryo formation and development. FOXF2, as a member of FOX family, It has been proved to play an important role in the occurrence and development of breast cancer and prostate cancer. However, the relationship between its expression and prognosis in HCC has not been reported in the literature. [objective] in this study, FOXF2 molecule was selected to detect the expression level of HCC and its relationship with prognosis, and the relationship between the expression of HCC molecule and the biological behavior of HCC cell line was studied. [methods] (1) Immunohistochemical method and real-time PCR were used to detect the expression of FOXF2 in 295 cases of HCC and matched paracancerous tissues and their correlation with clinicopathological parameters. (2) the expression of FOXF2 in 295 cases of HCC tissues and cells was detected by IHC,Western Blot and real-time PCR, and the correlation between FOXF2 expression and clinicopathological parameters was analyzed. (3) the expression of FOXF2 in MHCC-97H HCC cell line was interfered with by synthetic FOXF2 interference. To detect the effect of HCC on proliferation and apoptosis of HCC cells. [results] 1. (1) the expression level of FOXF2 in HCC tissues was lower than that in HCC tissues by IHC,Western Blot and real-time PCR. (1) the expression of FOXF2 in HCC tissues decreased, in addition, in HCC cell line MHCC-97H with strong metastasis and invasiveness. (2) the results of statistical analysis showed that the low expression of FOXF2 was positively correlated with the poor prognosis of HCC patients. (3) the preliminary study on the expression of FOXF2 in HCC cell line found that the expression of FOXF2 promoted the apoptosis of HCC cells and inhibited the proliferation of HCC cells. [conclusion] the decrease of 1.FOXF2 expression may play an important role in the occurrence and development of HCC. The expression of FOXF2 can be used as an independent predictor of postoperative survival in patients with HCC. 2. The decrease of FOXF2 expression can improve the anti-apoptosis ability of HCC cells and promote the proliferation of HCC cells.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R735.7
【参考文献】
相关期刊论文 前1条
1 ;Expression and clinical significance of TAp73α, p53, PCNA and apoptosis in hepatocellular carcinoma[J];World Journal of Gastroenterology;2005年18期
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