FK506结合蛋白5在肝细胞癌中的表达及其临床意义

发布时间：2019-06-17 17:34

【摘要】：目的:探讨FK506结合蛋白5(FKBP5)在肝细胞癌(HCC)中的表达及临床意义。方法:用q RT-PCR和Western blot法检测30对新鲜HCC及相应癌旁组织中FKBP5 m RNA和蛋白的表达;免疫组化法检测107例HCC患者石蜡标本中FKBP5的表达,并分析其与HCC临床病理特征及预后的关系。结果:30例新鲜HCC标本中,26例(86.7%)HCC组织FKBP5 m RNA的表达水平明显高于相应癌旁组织,HCC组织中FKBP5蛋白表达量明显高于其癌旁组织。石蜡标本分析显示,FKBP5蛋白在HCC组织中的高表达率明显高于癌旁组织(63.6%vs.28.0%,P0.001);FKBP5的表达水平与肿瘤大小(P=0.009)、肿瘤个数(P=0.018)、微血管侵犯(P=0.020)、TNM分期(P=0.009)和BCLC分期(P=0.024)有关,且FKBP5高表达患者术后总体生存率和无瘤生存率均明显低于低表达组(均P0.001);FKBP5高表达是HCC患者术后总体生存(HR=1.992,95%CI=1.127~3.522,P=0.018)和无瘤生存(HR=1.787,95%CI=1.067~2.992,P=0.027)的独立危险因素。结论:FKBP5在HCC组织中高表达,且与患者不良临床病理特征及预后密切相关,提示其是一个潜在的HCC预后分子标志物。
[Abstract]:Objective: to investigate the expression and clinical significance of FK506 binding protein 5 (FKBP5) in hepatocellular carcinoma (HCC). Methods: the expression of FKBP5 m RNA and protein in 30 pairs of fresh HCC and corresponding paracancerous tissues were detected by Q RT-PCR and Western blot, and the expression of FKBP5 in paraffin specimens of HCC patients was detected by immunohistochemistry, and the relationship between FKBP5 and clinicopathological features and prognosis of HCC was analyzed. Results: the expression level of FKBP5 m RNA in 26 (86.7%) HCC tissues was significantly higher than that in corresponding paracancerous tissues, and the expression of FKBP5 protein in HCC tissues was significantly higher than that in paracancerous tissues. Paraffin analysis showed that the high expression rate of FKBP5 protein in HCC was significantly higher than that in paracancerous tissues (63.6% vs. 28.0%, P0.001). The expression level of FKBP5 was correlated with tumor size (P 鈮，

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