乳腺癌和导管增生病变与17p染色体的遗传学不稳定关系

发布时间：2019-06-21 06:57

【摘要】：目的通过检测乳腺导管普通型增生(Usual Dutal Hyperplasia,UDH),非典型增生(Atypical Dutal Hyperplasia,ADH),导管原位癌(Dutal Carcinoma In Situ,DCIS)及浸润性导管癌(Invasive Dutal Carcinoma,IDC)中染色体17p微卫星不稳定(Microsatellite instability,MSI)/杂合性缺失(Loss Of Heterozygosity,LOH)的改变规律,探讨乳腺癌发展中其遗传学改变的特征。方法提取106例乳腺病变石蜡包埋组织(其中UDH 31例、ADH 10例、DCIS 35例、IDC 30例)的DNA,参考文献选取17p上D17S695、D17S261、D17S786、D17S796、D17S831、D17S654这6个微卫星位点,采用PCR方法扩增位点的DNA,然后用8%聚丙烯酰胺凝胶电泳,银染显色后进行LOH/MSI分析。结果 106例检测标本中,在DCIS、IDC组中发生频率要高于UDH组(P0.05)。在位点D17S695、D17S786、D17S796、D17S831上,各组LOH/MSI的发生频率差异均无统计学意义(P0.05);而在位点D17S261、D17S654上,各组LOH/MSI的发生频率差异均有统计学意义(P0.05)。进一步比较中D17S261上UDH组低于IDC组(P0.008);D17S654上UDH组低于DCIS组(P0.008)。结论 17P染色体的遗传学不稳定是乳腺癌发生的早期事件,D17S261、D17S654位点的改变可能在乳腺导管增生性病变进展为乳腺癌过程中起到关键作用。
[Abstract]:Objective to detect the changes of chromosome 17p microsatellite instability (Microsatellite instability,MSI) / loss of heterozygosity (Loss Of Heterozygosity,LOH) in breast cancer (Usual Dutal Hyperplasia,UDH), atypical hyperplasia (Atypical Dutal Hyperplasia,ADH), ductal carcinoma in situ (Dutal Carcinoma In Situ,DCIS) and invasive ductal carcinoma (Invasive Dutal Carcinoma,IDC), and to explore the characteristics of genetic changes in breast cancer. Methods six microsatellite sites, D17S695, D17S786, D17S796, D17S831 and D17S654, were extracted from paraffin-embedded tissues of 106 cases of breast lesions (including 31 cases of UDH, 10 cases of ADH, 35 cases of DCIS and 30 cases of IDC). The DNA, of the sites was amplified by PCR and then analyzed with 8% polyacrylamide gel electrophoresis and silver staining. Results the frequency of DCIS,IDC group was higher than that of UDH group (P 0.05). At loci D17S695, D17S786, D17S796, D17S831, there was no significant difference in the frequency of LOH/MSI among the three groups (P 0.05), but there was significant difference in the frequency of LOH/MSI in each group at D17S661 and D17S654 (P 0.05), but there was no significant difference in the frequency of LOH/MSI among the three groups at D17S695, D17S786, D17S796 and D17S831 (P 0.05). In further comparison, UDH group on D17S261 was lower than IDC group (P0.008), UDH group on D17S654 was lower than DCIS group (P0.008). Conclusion genetic instability of chromosome 17P is an early event in breast cancer. The changes of D17S261and D17S654 loci may play a key role in the progress of breast ductal hyperplasia into breast cancer.
【作者单位】：贵州医科大学病理学教研室;
【基金】：贵州省科学技术基金(黔科合J字2278) 贵州省社会发展攻关项目(黔科合SY字3016)
【分类号】：R737.9;R655.8

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