SRPX2在子宫内膜癌中的表达及对HEC-1A细胞增殖凋亡的影响
发布时间：2019-06-29 20:12
【摘要】：目的:探讨SRPX2在人子宫内膜癌中的表达及生物学功能。方法:收集50例子宫内膜癌及癌旁组织,免疫组化染色法检测SRPX2的表达,分析SRPX2表达与患者临床特点的关系。通过si RNA下调HEC-1A细胞中SRPX2的表达,检测HEC-1A细胞增殖及凋亡改变。检测沉默SRPX2后细胞内FAK及Cyclin D1表达改变。结果:SRPX2在子宫内膜癌组织中表达明显升高并与肿瘤直径增大及高FIGO分期(Ⅲ+Ⅳ期)显著相关(P0.05);沉默SRPX2表达可显著抑制HEC-1A细胞的增殖并促进凋亡,并导致细胞中FAK及Cyclin D1的表达降低。结论:子宫内膜癌组织中高表达的SRPX2与肿瘤增殖关系密切,沉默SRPX2可能通过下调FAK/Cyclin D1的表达而抑制子宫内膜癌细胞增殖并促进凋亡。
[Abstract]:Objective: to investigate the expression and biological function of SRPX2 in human endometrial carcinoma. Methods: the expression of SRPX2 was detected by immunohistochemical staining in 50 cases of endometrial carcinoma and paracancerous tissues, and the relationship between the expression of SRPX2 and the clinical characteristics of the patients was analyzed. The expression of SRPX2 in HEC-1A cells was down-regulated by si RNA, and the proliferation and apoptosis of HEC-1A cells were detected. The expression of FAK and Cyclin D1 in cells after silencing SRPX2 was detected. Results: the expression of SRPX2 was significantly increased in endometrial carcinoma and significantly correlated with the increase of tumor diameter and high FIGO stage (stage III IV) (P 0.05). The expression of silent SRPX2 could significantly inhibit the proliferation and promote apoptosis of HEC-1A cells, and lead to the decrease of the expression of FAK and Cyclin D1 in the cells. Conclusion: the high expression of SRPX2 in endometrial carcinoma is closely related to tumor proliferation. Silent SRPX2 may inhibit the proliferation and promote apoptosis of endometrial cancer cells by down-regulating the expression of FAK/Cyclin D1.
【作者单位】： 西南医科大学附属医院妇科;
【分类号】：R737.33
，
本文编号：2508077
[Abstract]:Objective: to investigate the expression and biological function of SRPX2 in human endometrial carcinoma. Methods: the expression of SRPX2 was detected by immunohistochemical staining in 50 cases of endometrial carcinoma and paracancerous tissues, and the relationship between the expression of SRPX2 and the clinical characteristics of the patients was analyzed. The expression of SRPX2 in HEC-1A cells was down-regulated by si RNA, and the proliferation and apoptosis of HEC-1A cells were detected. The expression of FAK and Cyclin D1 in cells after silencing SRPX2 was detected. Results: the expression of SRPX2 was significantly increased in endometrial carcinoma and significantly correlated with the increase of tumor diameter and high FIGO stage (stage III IV) (P 0.05). The expression of silent SRPX2 could significantly inhibit the proliferation and promote apoptosis of HEC-1A cells, and lead to the decrease of the expression of FAK and Cyclin D1 in the cells. Conclusion: the high expression of SRPX2 in endometrial carcinoma is closely related to tumor proliferation. Silent SRPX2 may inhibit the proliferation and promote apoptosis of endometrial cancer cells by down-regulating the expression of FAK/Cyclin D1.
【作者单位】： 西南医科大学附属医院妇科;
【分类号】：R737.33
，
本文编号：2508077
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