Tspan9通过下调整合素β1的表达抑制胃癌SGC7901细胞的迁移与侵袭
发布时间：2019-06-29 22:47
【摘要】：研究目的:1、研究四跨膜蛋白Tspan9对胃癌SGC7901细胞迁移和侵袭的影响;2、观察Tspan9对整合素β1蛋白表达的影响;3、验证Tspan9是否通过整合素β1影响SGC7901细胞的迁移和侵袭。研究方法:1、首先检测前期通过慢病毒转染Tspan9的T-SGC7901细胞中Tspan9蛋白的表达情况;2、通过划痕实验和transwell实验分别检测Tspan9对SGC7901细胞迁移和侵袭的影响;3、通过脂质体瞬时转染整合素β1,建立ITG/T-SGC7901细胞,并检测转染后整合素β1的表达情况;4、再次通过细胞划痕实验和transwell实验检测转染整合素β1后SGC7901细胞迁移和侵袭能力的变化。结果:1、与对照组相比,T-SGC7901细胞Tspan9蛋白的表达明显增多(P0.05);2、划痕实验显示,T-SGC7901细胞的迁移率较对照组明显降低(P0.05),transwell实验显示,T-SGC7901细胞侵入到下室的细胞数量较对照组明显减少(P0.05);3、转染整合素β1后,ITG/T-SGC7901细胞整合素β1的表达明显增多;4、划痕实验显示,ITG/T-SGC7901细胞的迁移率较对照组明显加快(P0.05),transwell实验显示,ITG/T-SGC7901细胞侵入到下室的细胞数量较对照组明显增多(P0.05)。结论和意义:Tspan9能够抑制胃癌细胞SGC7901的迁移和侵袭能力,这种抑制作用可能是通过下调整合素β1表达实现的。本研究进一步加深了对四跨膜蛋白和恶性肿瘤关系的认识,为后续研究提供实验基础。
[Abstract]:Aim: 1. To study the effect of four transmembrane protein Tspan9 on the migration and invasion of gastric cancer SGC7901 cells; 2, to observe the effect of Tspan9 on the expression of integrin 尾 1 protein; and 3. To verify whether Tspan9 affects the migration and invasion of SGC7901 cells through integrin 尾 1. Methods: firstly, the expression of Tspan9 protein in T-SGC7901 cells transfected with lentivirus Tspan9 was detected. 2, the effects of Tspan9 on the migration and invasion of SGC7901 cells were detected by scratch test and transwell assay, respectively. 3, ITG/T-SGC7901 cells were established by transient transfer of integrin 尾 1 by liposomes, and the expression of integrin 尾 1 after transfection was detected. 4. The migration and invasiveness of SGC7901 cells were detected by cell scratch test and transwell assay. Results: 1, compared with the control group, the expression of Tspan9 protein in T-SGC7901 cells was significantly increased (P 0.05). 2, scratch test showed that the mobility of T-SGC7901 cells was significantly lower than that in the control group (P 0.05), transwell test). 3, the expression of integrin 尾 1 in ITG/T-SGC7901 cells was significantly increased after transfection of integrin 尾 1 compared with the control group (P 0.05), and the expression of integrin 尾 1 in ITG/T-SGC7901 cells was significantly higher than that in the control group (P 0.05), and the expression of integrin 尾 1 in ITG/T-SGC7901 cells was significantly higher than that in the control group (P 0.05). 4, scratch test showed that the mobility of ITG/T-SGC7901 cells was significantly faster than that of the control group (P 0.05), transwell test showed that the number of ITG/T-SGC7901 cells invading the inferior chamber was significantly higher than that of the control group (P 0.05). Conclusion and significance: Tspan9 can inhibit the migration and invasion of SGC7901 in gastric cancer cells, which may be achieved by down-regulating the expression of integrin 尾 1. This study further deepened the understanding of the relationship between four transmembrane proteins and malignant tumors, and provided an experimental basis for further study.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.2
本文编号：2508183
[Abstract]:Aim: 1. To study the effect of four transmembrane protein Tspan9 on the migration and invasion of gastric cancer SGC7901 cells; 2, to observe the effect of Tspan9 on the expression of integrin 尾 1 protein; and 3. To verify whether Tspan9 affects the migration and invasion of SGC7901 cells through integrin 尾 1. Methods: firstly, the expression of Tspan9 protein in T-SGC7901 cells transfected with lentivirus Tspan9 was detected. 2, the effects of Tspan9 on the migration and invasion of SGC7901 cells were detected by scratch test and transwell assay, respectively. 3, ITG/T-SGC7901 cells were established by transient transfer of integrin 尾 1 by liposomes, and the expression of integrin 尾 1 after transfection was detected. 4. The migration and invasiveness of SGC7901 cells were detected by cell scratch test and transwell assay. Results: 1, compared with the control group, the expression of Tspan9 protein in T-SGC7901 cells was significantly increased (P 0.05). 2, scratch test showed that the mobility of T-SGC7901 cells was significantly lower than that in the control group (P 0.05), transwell test). 3, the expression of integrin 尾 1 in ITG/T-SGC7901 cells was significantly increased after transfection of integrin 尾 1 compared with the control group (P 0.05), and the expression of integrin 尾 1 in ITG/T-SGC7901 cells was significantly higher than that in the control group (P 0.05), and the expression of integrin 尾 1 in ITG/T-SGC7901 cells was significantly higher than that in the control group (P 0.05). 4, scratch test showed that the mobility of ITG/T-SGC7901 cells was significantly faster than that of the control group (P 0.05), transwell test showed that the number of ITG/T-SGC7901 cells invading the inferior chamber was significantly higher than that of the control group (P 0.05). Conclusion and significance: Tspan9 can inhibit the migration and invasion of SGC7901 in gastric cancer cells, which may be achieved by down-regulating the expression of integrin 尾 1. This study further deepened the understanding of the relationship between four transmembrane proteins and malignant tumors, and provided an experimental basis for further study.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.2
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