肿瘤/睾丸抗原REC8在结直肠癌进展中的作用及机制
发布时间：2021-03-01 01:08
结直肠癌是消化道常见的恶性肿瘤之一,其发病率高,死亡率高,严重威胁国民健康。结直肠癌起病隐匿,早期症状不典型,许多患者就诊时已发生转移。合并转移的结直肠癌患者预后不良,1年相对生存率为52.6%,2年相对生存率仅为33.3%。结直肠癌目前主要的治疗方式为手术切除和放化疗。这些治疗手段一般初期疗效较好,但经过数月至数年的缓解后,约30-50%的患者病情进展,发生肿瘤侵袭和转移。而结直肠癌患者的主要死亡原因正是侵袭和转移。因此,防治肿瘤进展（主要表现为侵袭和转移）是提高患者生存率的重要手段。肿瘤进展是一个复杂的过程,受多种因素的调控,目前认为上皮-间质转化（Epithelial-mesenchymal transition,EMT）和肿瘤干细胞（Cancer stem cells,CSCs）与肿瘤转移密切相关。肿瘤DNA倍体异常是肿瘤进展及恶性度的标志。上皮-间质转化是指上皮细胞通过特定程序转化为具有间质表型细胞的过程。EMT涉及Snail、Slug、Twist等一系列多能转录因子的变化,这些变化引起细胞粘附分子（如E-钙粘蛋白）表达的减少,基质降解酶表达的增加,细胞运动性的增加和对凋亡抗...
【文章来源】：河北医科大学河北省
【文章页数】：62 页
【学位级别】：硕士
【部分图文】：
REC8在结直肠癌组织及其配对癌旁组织中的表达水平无显著差异（P=0.761）
28 3 过表达REC8可促进MMP-（9P=0.016, P=0.017）及VEGF-C（P=0.01P=0.009）的表达Fig. 3 Overexpression of REC8 promotes the expression of MMP-9 andVEGF-C at protein level, b) Western blotting for MMP-9 and VEGF-C in human colon carcinomll lines (a) HCT116 and (b) SW480. GAPDH was used as loading controar graphs represented means ± SEM from at least three independeperiment; *P＜0.05, **P＜0.01, ***P＜0.001 vs. indicated groups; ndicates not significant.
图 4 过表达 REC8 组 PCNA 的表达水平与对照组相比差异无统计学意（P=0.12, P=0.492）Fig. 4 Overexpression of REC8 does not affect the expression of PCNA aprotein level(a, b) Western blotting for PCNA in human colon carcinoma cell linesHCT116 and (b) SW480. GAPDH was used as loading control. Bar grarepresented means±SEM from at least three independent experiment; *P0.05, **P ＜ 0.01, ***P ＜ 0.001 vs. indicated groups; n.s. indicates significant.
本文编号：3056725
【文章来源】：河北医科大学河北省
【文章页数】：62 页
【学位级别】：硕士
【部分图文】：
REC8在结直肠癌组织及其配对癌旁组织中的表达水平无显著差异（P=0.761）
28 3 过表达REC8可促进MMP-（9P=0.016, P=0.017）及VEGF-C（P=0.01P=0.009）的表达Fig. 3 Overexpression of REC8 promotes the expression of MMP-9 andVEGF-C at protein level, b) Western blotting for MMP-9 and VEGF-C in human colon carcinomll lines (a) HCT116 and (b) SW480. GAPDH was used as loading controar graphs represented means ± SEM from at least three independeperiment; *P＜0.05, **P＜0.01, ***P＜0.001 vs. indicated groups; ndicates not significant.
图 4 过表达 REC8 组 PCNA 的表达水平与对照组相比差异无统计学意（P=0.12, P=0.492）Fig. 4 Overexpression of REC8 does not affect the expression of PCNA aprotein level(a, b) Western blotting for PCNA in human colon carcinoma cell linesHCT116 and (b) SW480. GAPDH was used as loading control. Bar grarepresented means±SEM from at least three independent experiment; *P0.05, **P ＜ 0.01, ***P ＜ 0.001 vs. indicated groups; n.s. indicates significant.
本文编号：3056725
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