miR-342-3p对肝细胞癌糖酵解的调控作用与机制的研究

发布时间：2021-06-17 13:45

　　肝细胞癌（hepatocellular carcinoma,HCC）是肝脏最常见的恶性肿瘤,在世界范围内普遍流行,最新的流行病学调查显示,其发病率位于所有恶性肿瘤中第五位,肿瘤致死性疾病中第三位,并且发病率具有一定的性别差异,于男性中排名第三位,女性中第八位。HCC生长速度快、恶性程度高,常伴有血管侵犯、肝内及肝外转移、治疗后复发等,预后差,其5年生存率仅仅为3%-25%,严重危害着人类的健康。目前仍未完全阐明HCC的发病机制,因此,探究新的分子靶点及信号通路具有重要的科研及临床意义。Micro RNAs（miRNAs）是一类单链的,长度约为14-24个核苷酸,非编码的小RNA片段,可以特异性结合至靶基因的3’末端非翻译区（3’end of the untranslated regions,3’-UTR）,导致靶基因的降解或抑制靶基因的翻译,从而调控多个生理及病理过程,例如细胞增殖、周期、分化、凋亡等基本生命过程。大量的研究结果显示,miRNA在肿瘤的发生发展过程中扮演了促癌或抑癌的角色,miRNA的功能失调会影响肿瘤的发生及进展。miRNA可能成为肿瘤诊断、治疗的一个潜在的小分子生物... 

【文章来源】：河北医科大学河北省

【文章页数】：125 页

【学位级别】：博士

【部分图文】：

糖酵解在miR-342-3p抑制HepG2细胞增殖中发挥了重要作用Fig.1miR-342-3psuppressedthecellproliferationandcolonyformationabilityinHepG2hepatomacells.2-deoxy-D-glucose(2-DG),theglycolyticinhibitor,impairedtheabilityofmiR-342-3pmimicstoinhibitproliferation

26图 2 糖酵解在miR-342-3p抑制MHCC97H细胞增殖中发挥了重要作用Fig. 2 miR-342-3p suppressed the cell proliferation and colony formationability in MHCC97H hepatoma cells. 2-deoxy-D-glucose (2-DG), theglycolytic inhibitor, impaired the ability of miR-342-3p mimics to inhibitproliferation and colony formation in MHCC97H hepatoma cells.**P < 0.01

1 IGF-1R 是 miR-342-3p 的靶 target of miR-342-3p in HEKells transfected with NC or mve control. β–actin was used a

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