Wnt4/JNK1信号通路在食管鳞癌中表达的研究
发布时间：2021-10-21 18:25
背景和目的:食管癌是常见的致死率较高的消化道恶性肿瘤之一,我国食管癌约90%属于鳞状细胞癌。Wnt信号通路在调控基因表达及细胞生物学行为中发挥重要作用。Wnt4属于非经典Wnt信号分子并且可调控上皮细胞分化,其表达异常与人类鳞状细胞癌具有相关性。JNK1作为非经典wnt信号通路中位于细胞内的重要信使分子,可介导肿瘤细胞的凋亡。本实验旨在研究非经典Wnt信号通路中Wnt4、JNK1在食管鳞癌组织中的表达情况及其与临床病理参数的关系,探讨其在食管鳞癌发生发展中的意义。方法:收集、整理临床资料及食管鳞癌、癌旁组织标本;使用半定量PCR的方法检测Wnt4、JNK1基因在食管鳞癌及癌旁组织中的m RNA表达情况;使用免疫组织化学染色方法检测Wnt4及JNK1蛋白在食管鳞癌及癌旁组织中的表达情况;对Wnt4、JNK1表达水平综合临床病理资料进行相关性研究,分析Wnt4/JNK1信号通路与食管鳞癌分化程度、病理分期、淋巴转移、浸润深度之间的关系。结果:（1）食管鳞癌组织中Wnt4、JNK1 m RNA表达水平低于癌旁正常组织,差异具有统计学意义（P<0.05）。食管鳞癌高分化组中Wnt4 m ...
【文章来源】：天津医科大学天津市 211工程院校
【文章页数】：76 页
【学位级别】：硕士
【部分图文】：
JNK1半定量PCR产物分子大小为355bp，凝胶电泳图见下
其中M为
内参（GAPDH）半定量PCR产物大小为156bp，凝胶电泳图见下
【参考文献】：
期刊论文
[1]中国食管癌流行病学现状、诊疗现状及未来对策[J]. 赫捷,邵康. 中国癌症杂志. 2011(07)
[2]p-JNK JNK和PPARγ在食管癌变过程中的表达及其意义[J]. 丁涛,张祥宏,李学民,严霞,邢凌霄,王俊灵,张杰英,薛丽英,赵晨燕,崔晋峰. 中国肿瘤临床. 2008(23)
[3]Expression level of beta-catenin is associated with prognosis of esophageal carcinoma[J]. Lv Ji, Xiu-Feng Cao, He-Ming Wang, Yi-Sheng Li, Bin Zhu, Jian Xiao, Dan Wang Affillted Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, Jiangsu Province, China. World Journal of Gastroenterology. 2007(18)
[4]Role of JNK activation in apoptosis:Adouble-edged sword[J]. Jing LIU, Anning LIN* Ben May Institute for Cancer Research, The University of Chicago, 5841 S. Maryland Avenue, MC 6027, Chicago, IL 60637, USA. Cell Research. 2005(01)
[5]Expression of e-cadherin and β-catenin in human esophageal squamous cell carcinoma: relationships with prognosis[J]. Xi-Jiang Zhao Qing-Lai Feng Department of Thoracic Surgery, Cancer Hospital, Tianjin Medical University, Tianjin 300060, ChinaHui Li Department of Thoracic Surgery, Shandong Cancer Hospital & Institute, Jinan 250117, Shandong Province, ChinaHua Chen Yan-Xue Liu Li-Hua Zhang Su-Xiang Liu Department of Pathology, Cancer Hospital & Institute, Tianjin Medical University, Yianjin 300060, China. World Journal of Gastroenterology. 2003(02)
[6]Gene expression profiles at different stages of human esophageal squamous cell carcinoma[J]. Jin Zhou Xiu-Qin Wang Min Wu Zhi-Hua Liu National Laboratory of Molecular Oncology,Cancer Institute,Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 100021,China Li-Qun Zhao Guan-Rui Yang Zong-Liang Qiu Medical Science Institute of Henan Province,Zhengzhou 450052,China Mo-Miao Xiong Health Genetics Center,School of Public Health,The University of Texas Houston,Houston,Texas 77225,USA. World Journal of Gastroenterology. 2003(01)
本文编号：3449501
【文章来源】：天津医科大学天津市 211工程院校
【文章页数】：76 页
【学位级别】：硕士
【部分图文】：
JNK1半定量PCR产物分子大小为355bp，凝胶电泳图见下
其中M为
内参（GAPDH）半定量PCR产物大小为156bp，凝胶电泳图见下
【参考文献】：
期刊论文
[1]中国食管癌流行病学现状、诊疗现状及未来对策[J]. 赫捷,邵康. 中国癌症杂志. 2011(07)
[2]p-JNK JNK和PPARγ在食管癌变过程中的表达及其意义[J]. 丁涛,张祥宏,李学民,严霞,邢凌霄,王俊灵,张杰英,薛丽英,赵晨燕,崔晋峰. 中国肿瘤临床. 2008(23)
[3]Expression level of beta-catenin is associated with prognosis of esophageal carcinoma[J]. Lv Ji, Xiu-Feng Cao, He-Ming Wang, Yi-Sheng Li, Bin Zhu, Jian Xiao, Dan Wang Affillted Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, Jiangsu Province, China. World Journal of Gastroenterology. 2007(18)
[4]Role of JNK activation in apoptosis:Adouble-edged sword[J]. Jing LIU, Anning LIN* Ben May Institute for Cancer Research, The University of Chicago, 5841 S. Maryland Avenue, MC 6027, Chicago, IL 60637, USA. Cell Research. 2005(01)
[5]Expression of e-cadherin and β-catenin in human esophageal squamous cell carcinoma: relationships with prognosis[J]. Xi-Jiang Zhao Qing-Lai Feng Department of Thoracic Surgery, Cancer Hospital, Tianjin Medical University, Tianjin 300060, ChinaHui Li Department of Thoracic Surgery, Shandong Cancer Hospital & Institute, Jinan 250117, Shandong Province, ChinaHua Chen Yan-Xue Liu Li-Hua Zhang Su-Xiang Liu Department of Pathology, Cancer Hospital & Institute, Tianjin Medical University, Yianjin 300060, China. World Journal of Gastroenterology. 2003(02)
[6]Gene expression profiles at different stages of human esophageal squamous cell carcinoma[J]. Jin Zhou Xiu-Qin Wang Min Wu Zhi-Hua Liu National Laboratory of Molecular Oncology,Cancer Institute,Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 100021,China Li-Qun Zhao Guan-Rui Yang Zong-Liang Qiu Medical Science Institute of Henan Province,Zhengzhou 450052,China Mo-Miao Xiong Health Genetics Center,School of Public Health,The University of Texas Houston,Houston,Texas 77225,USA. World Journal of Gastroenterology. 2003(01)
本文编号：3449501
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