儿童病毒性肺炎风寒郁肺证代谢组学研究

发布时间：2017-12-26 22:32

本文关键词：儿童病毒性肺炎风寒郁肺证代谢组学研究　出处：《南京中医药大学学报》2017年02期 　论文类型：期刊论文

【摘要】：目的分析病毒性肺炎风寒郁肺证患儿的血清代谢指纹图谱,探讨中医证候的组学代谢物研究方法。方法 30例风寒郁肺证患儿、30例健康儿童血清进行气相色谱-质谱检测,通过在线分析平台XCMS Online预处理、NIST数据库和已有标准品进行验证、MetaboAnalyst确定差异性代谢物及代谢通路。结果有机酸、氨基酸、脂类等差异性代谢物及代谢通路的确定,能够良好区分风寒郁肺证与健康儿童组。与健康儿童相比,风寒郁肺证患儿血清中丙氨酸、天冬氨酸、谷氨酸、甘氨酸、丝氨酸、苏氨酸、牛磺酸、赖氨酸等多种有机酸、氨基酸上调;氨基丁酸、乙醇酸、甘油、1-棕榈酸单甘油酯、胆固醇、葡萄糖等下调,主要涉及丙氨酸、甘氨酸、牛磺酸代谢、氨酰tRNA合成和赖氨酸降解等5条代谢通路异常。结论病毒性肺炎风寒郁肺证存在差异代谢物及代谢通路,这可能与病毒感染导致的机体的多个能量代谢通路相关;代谢组学可为类似中医证候客观化研究提供借鉴。
[Abstract]:Objective to analyze the serum metabolic fingerprints of children with wind cold and depressed lung syndrome in viral pneumonia, and to explore the research methods of the metabolites of TCM syndromes. Methods serum samples from 30 cases of chill, lung depression and 30 healthy children were detected by gas chromatography-mass spectrometry. The differential metabolites and metabolic pathways were determined by online analysis platform XCMS Online pretreatment, NIST database and existing standard products, MetaboAnalyst. The results of organic acids, amino acids, lipid differences of metabolites and metabolic pathways, can well differentiate cold obstructing lung syndrome and healthy children group. Compared with healthy children, children with cold obstructing lung syndrome serum alanine, aspartic acid, glutamic acid, glycine, serine, threonine, taurine, lysine and other organic acids and amino acids increased; amino butyric acid, glycolic acid, glycerol, 1- palmitic acid monoglyceride, cholesterol, glucose reduction, mainly involving alanine glycine, taurine, metabolism and aminoacyl tRNA synthesis and degradation of lysine 5 pathway abnormalities. Conclusion there are different metabolites and metabolic pathways in viral pneumonia, wind cold and lung depression syndrome, which may be related to multiple energy metabolism pathways induced by virus infection. Metabonomics can provide reference for similar TCM syndrome objectification research.
【作者单位】：江苏省儿童呼吸疾病(中医药)重点实验室南京中医药大学中医儿科学研究所;南京中医药大学医学代谢组学中心;河南中医药大学第一附属医院;美国加州大学戴维斯分校基因组中心NIH西海岸代谢组学研究中心;
【基金】：国家自然科学基金(81378688)
【分类号】：R272
【正文快照】： 组中心,NIH西海岸代谢组学研究中心,美国戴维斯95616)代谢组学是研究生物体受病理生理刺激或基因改变而引起的体内代谢物动态变化的一门学科[1]。代谢组学主要应用于疾病诊断、药物安全性评价、药物作用机制及植物代谢等方面的研究[2]。代谢指纹(Metabolic fingerprinting)即

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