珍宝类藏族药珍珠丸系列品种中挥发性成分的GC特征图谱

发布时间：2017-12-28 05:29

本文关键词：珍宝类藏族药珍珠丸系列品种中挥发性成分的GC特征图谱　出处：《中国实验方剂学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的:建立藏族药珍珠丸系列品种(二十五味珍珠丸、七十味珍珠丸)中挥发性成分的特征图谱,对其部分成分归属进行初步判定,为其全面质量评价提供依据。方法:Agilent DB-1毛细管柱(0.25 mm×30 m,0.25μm),进样口温度200℃,FID检测器温度320℃;柱温初始温度为60℃,保留1 min,以4℃·min~(-1)的速率升至90℃,保留1 min,再以2℃·min~(-1)的速率升至110℃,保留10 min,再以2.5℃·min~(-1)的速率升至200℃,保留5 min,最后以5℃·min~(-1)的速率升至280℃,保留8 min;载气氮气,流速3 m L·min~(-1),进样量2μL,分流比为5∶1。结果:GC特征图谱中标定的共有峰,精密度、稳定性、重复性均达到了要求(RSD5.0%),相似度分析中,不同厂家、不同批次的样品各成分的种类差异不大,但成分的比例和量上差异较大。结论:建立的气相特征图谱中,各共有峰分离较好,可为藏族药珍珠丸系列品种的质量评价提供借鉴。
[Abstract]:Objective: to establish the characteristic chromatogram of the volatile components in the series of Tibetan medicine Pearl Ball Series (twenty-five flavor pearl pills and seventy flavor pearl pills), and preliminarily determine their partial components, so as to provide basis for their comprehensive quality evaluation. Method: Agilent DB-1 column (0.25 mm * 30 m, 0.25 m), the inlet temperature of 200 DEG C, FID detector temperature of 320 DEG C; column temperature and initial temperature of 60 DEG C, keep 1 to 4 degrees min, min~ (-1) rate to 90 DEG C, retention of 1 min, and then to 2 C - min~ (-1) the rate of up to 110 degrees Celsius, retention of 10 min, and then to 2.5 DEG C - min~ (-1) the rate of retention of 5 to 200 DEG C, min, finally to 5 DEG C - min~ (-1) rate to 280 DEG C, retained 8 min; nitrogen carrier gas, the flow rate of 3 m L. Min~ (-1), sample volume 2 L, the split ratio is 5: 1. Results: the common peaks in the GC characteristic map reached the requirement of accuracy, stability and repeatability (RSD5.0%). In similarity analysis, there was little difference in the components among different manufacturers and batches of samples, but the proportion and quantity of components were quite different. Conclusion: the common peaks are well separated in the gas phase characteristics atlas established, which can be used for reference for the quality evaluation of the Tibetan medicine Pearl Pill series.
【作者单位】：青海省药品检验检测院;青海省食品药品监督管理局食品药品审评中心;
【基金】：青海省(基础)研究计划项目(2013-Z-716)
【分类号】：R29
【正文快照】： [网络出版地址]http://kns.cnki.net/kcms/detail/11.3495.R.20170308.0953.042.html[网络出版时间]2017-03-08 9:53二十五味珍珠丸系藏族验方,最早收载于《四部医典》的第三部“秘诀部”中[1],现收载于《中国药典》2015年版一部,由珍珠、肉豆蔻、石灰华、草果、丁香、西红花

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