缺血性中风病患者体质、证候与MMP-9,IL-6,MTHFR基因多态性的相关性研究

发布时间：2017-12-28 09:15

本文关键词：缺血性中风病患者体质、证候与MMP-9,IL-6,MTHFR基因多态性的相关性研究　出处：《中国中药杂志》2017年18期 　论文类型：期刊论文

【摘要】：通过研究缺血性中风病患者证候、体质与MMP-9,IL-6,MTHFR基因多态性之间相互关系,对缺血性中风病患者从体质、证候与MMP-9,IL-6,MTHFR基因多态性关系进行分析。采集缺血性中风病患者的相关资料,建立数据库,进行统计分析。结果纳入的61例缺血性中风病患者中风痰瘀阻证患者出现频率最高,共30例;痰湿质是缺血性中风病患者易发体质,共20例;体质与证候对应关系分析显示:气虚体质患者发病后倾向表现为气虚血瘀证;痰湿体质和平和体质患者发病后倾向表现为风痰瘀阻证;证候与MMP-9,IL-6关系:风痰瘀阻证、气虚血瘀证患者MMP-9,IL-6分布经Z检验有显著统计学差异(P0.05),气虚血瘀证患者MMP-9水平明显高于风痰瘀阻证患者;风痰瘀阻证患者IL-6水平明显高于气虚血瘀证患者;证候、体质与MTHFR基因多态性关系:61个样本中,共检测到杂合突变34例;纯和突变15例;无突变12例,该基因位点突变率是未突变患者的4.08倍;痰湿质患者发病后其MTHFR C677T基因型倾向于表现为CT基因型;风痰瘀阻证患者发病后易表现为TT基因型;阴虚动风证患者发病后易发生杂和突变,表现为CT基因型。从缺血性中风病患者证候与体质关系分析显示,痰湿质、平和质患者发病后易表现为风痰瘀阻证;气虚质患者发病后易表现为气虚血瘀证。提示发病前如对于偏颇体质进行调理,或许对预防缺血性中风病有一定效果;从缺血性中风病患者证候与MMP-9,IL-6关系分析显示,风痰瘀阻证与IL-6水平具有相关性,气虚血瘀证与MMP-9水平具有相关性。从缺血性中风病患者证候与MTHFR基因多态性关系分析显示,TT基因型患者发病后易表现为风痰瘀阻证,CT基因型患者发病后易表现为阴虚动风证;从缺血性中风病患者体质与MTHFR基因多态性关系分析显示,CT基因型患者易表现为痰湿质。为更深入地认识缺血性中风病病机提供依据,为临床治疗与预防提供干预策略。
[Abstract]:By studying the relationship between syndromes, physical fitness and MMP-9, IL-6 and MTHFR gene polymorphisms in ischemic stroke patients, we analyzed the relationship between constitution, syndrome and MMP-9, IL-6 and MTHFR gene polymorphisms in ischemic stroke patients. The related data of patients with ischemic stroke were collected, and a database was set up to carry out statistical analysis. Results in 61 cases of ischemic stroke in patients with stroke, phlegm and blood stasis syndrome in patients with the highest frequency, a total of 30 cases; phlegm dampness is patients with ischemic stroke prone physique, a total of 20 cases; analysis of constitution and syndrome relationship: the constitution of qi deficiency patients after onset of tendency for Qi and blood stasis syndrome; phlegm dampness constitution peace and health after the onset of tendency to wind phlegm and blood stasis syndrome; IL-6 syndrome and MMP-9: wind phlegm and blood stasis syndrome, Qi deficiency and blood stasis syndrome in patients with MMP-9, IL-6 distribution by Z test with significant difference (P0.05), Qi deficiency and blood stasis syndrome in patients with MMP-9 were significantly higher than that in the water wind phlegm and blood stasis syndrome; the level of IL-6 in patients with wind phlegm and blood stasis syndrome was significantly higher than that of patients with Qi deficiency and blood stasis syndrome; constitution, the association between MTHFR gene polymorphism and 61 samples were detected in 34 cases with heterozygous mutation; homozygous mutation in 15 cases; 12 cases without mutation, the mutation rate is not mutation in 4. 08 times. After the onset of phlegm dampness, the genotype of MTHFR C677T tended to be CT genotype. The patients with wind phlegm and blood stasis syndrome were prone to be TT genotype, and yin deficiency and dynamic wind syndrome were prone to be heterozygous and mutation, showing CT genotype. From the analysis of the relationship between syndromes and physical fitness of patients with ischemic stroke, it is shown that phlegm dampness and mild constitution are easily manifested as wind, phlegm and blood stasis syndrome. Prompt before the onset of such as biased constitution conditioning, may have some effect on the prevention of ischemic stroke; syndrome and MMP-9 syndrome in patients with ischemic stroke, IL-6 analysis showed that the wind phlegm and blood stasis syndrome is associated with the level of IL-6, Qi deficiency and blood stasis syndrome and the level of MMP-9 has correlation. From the analysis of relationship between syndrome and MTHFR syndrome in patients with ischemic stroke incidence showed polymorphism, TT genotype is easy after the wind phlegm and blood stasis syndrome, the incidence of CT genotype patients after easy performance for dynamic wind from Yin deficiency syndrome; relationship between ischemic stroke patients with physical and MTHFR gene polymorphism analysis showed that CT genotype patients showed phlegm dampness. It provides a basis for understanding the pathogenesis of ischemic apoplexy, and provides an intervention strategy for clinical treatment and prevention.
【作者单位】：中国中医科学院中医临床基础医学研究所;中国中医科学院医学实验中心;北京六合华大基因科技有限公司武汉分公司;
【基金】：2015年度中医药行业科研专项项目(201507003-8)
【分类号】：R255.2
【正文快照】： 3.北京六合华大基因科技有限公司武汉分公司,湖北武汉430000)缺血性中风病是指因脑部血液供应障碍,缺血、缺氧所致的局限性脑组织的缺血性坏死或软化,近年来缺血性中风病发病率、死亡率逐年升高,全球疾病负担研究报告指出,自2010年来缺血性中风病成为导致中国居民死亡主要原因

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