回药木香油方对大鼠脑缺血再灌注损伤的治疗作用及其机制研究

发布时间：2017-12-28 14:31

本文关键词：回药木香油方对大鼠脑缺血再灌注损伤的治疗作用及其机制研究　出处：《宁夏医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:研究回药木香油方对大鼠脑缺血再灌注损伤的治疗作用及其作用机制。方法:雄性SD大鼠,体重300±20g,采用线栓法建立MCAO模型,缺血2小时再灌注后进行神经功能学评分,按照评分分为如下6组,即假手术组、模型对照组、尼莫地平对照组(12mg/kg)、木香油方低剂量组(4.0g/kg)、木香油方中剂量组(8.0g/kg)和木香油方高剂量组(16.0g/kg)。大鼠再灌注后且意识清醒时,采用Zea Longa 5级评分法评价神经缺失症状(计0时),然后于缺血后每日早8时进行评分。大鼠再灌注后4小时给药一次,然后于缺血后每日早9时给药,连续7天,假手术对照组、模型对照组给予溶媒(0.5%的CMC-Na)(1mL/100g体重),第7天给药两小时后,采血、处死动物后取脑,检测相关指标。按试剂盒方法检测各组大鼠血清中SOD、LDH、CAT、MDA、T-AOC和GSH-PX等指标,采用TTC染色法检测脑梗死面积,采用Western-blot法检测大鼠脑组织Cyt-c,Bax,Bcl-2,Caspase-3,Caspase-7,Caspase-9的蛋白表达。结果:实验发现木香油方能够显著的降低神经功能学评分,且可以有效减小脑梗死面积。大鼠血清中氧化应激指标显示,木香油方可使脑缺血再灌注损伤大鼠血清中SOD、GSH-PX、T-AOC、CAT增高,MDA、LDH含量下降。Western-blot结果显示,木香油方能够显著提高抗凋亡蛋白Bcl-2的表达,同时降低促凋亡蛋白Bax,Cyt-c,Caspase-3,Caspase-7,Caspase-9的表达。结论:木香油方对大鼠脑缺血再灌注损伤具有改善作用,作用机制可能与其抗氧化应激、抑制凋亡蛋白表达有关。
[Abstract]:Objective: To study the therapeutic effect and mechanism of Hui Yao Mu Xiang oil prescription on cerebral ischemia reperfusion injury in rats. Methods: male SD rats, weight 300 + 20g, MCAO model was established by using suture method, 2 hours after reperfusion of ischemic neurological function scores, according to the score is divided into the following 6 groups: sham operation group, model group, nimodipine group (12mg/kg), alantol side low dose group (4.0g/kg), dose alantol Fang (8.0g/kg) and alantol high dose group (16.0g/kg). After reperfusion and conscious, the Zea Longa 5 grade score was used to evaluate the symptoms of neurodeletions (0 hours), and then 8 hours after the ischemia. The rats were administrated once a day after reperfusion for 4 hours, then administrated for 9 days after the onset of ischemia for 7 days. The sham operated control group and the model control group were given the solvent (0.5% CMC-Na) (1mL/100g body weight). After two days of seventh days' administration, the animals were taken blood and killed, and then the brain was detected, and the related indexes were detected. The indexes of serum SOD, LDH, CAT, MDA, T-AOC and GSH-PX in each group were detected by kit method. The area of cerebral infarction was detected by TTC staining, and the protein expression of Cyt-c, Bax, Bcl-2, Bax, Western-blot in rats brain was detected by Western-blot. Results: it was found that wood oil could significantly reduce the score of neurologic function and reduce the area of cerebral infarction. The oxidative stress index in rat serum showed that the SOD, GSH-PX, T-AOC and CAT increased in serum and the content of MDA and LDH decreased in rats serum after cerebral ischemia reperfusion injury. Western-blot results showed that costusin oil could significantly increase the expression of anti apoptotic protein Bcl-2, and decrease the expression of Pro apoptotic protein Bax, Cyt-c, Caspase-3, Caspase-7 and Caspase-9. Conclusion: the effect of Mu Xiang oil on cerebral ischemia reperfusion injury in rats may be related to the anti oxidative stress and the inhibition of the expression of apoptotic protein.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R29

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