针刺内关穴对MCAO大鼠星形胶质细胞谷氨酸代谢通路的影响及机制研究

发布时间：2017-12-28 23:11

本文关键词：针刺内关穴对MCAO大鼠星形胶质细胞谷氨酸代谢通路的影响及机制研究　出处：《山东中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:从星形胶质细胞谷氨酸代谢通路的角度,初步探讨针刺对MCAO大鼠谷氨酸-谷氨酰胺循环关键环节谷氨酸转运体1(GLT1)和谷氨酰胺合成酶(GS)的调控作用,为针刺治疗缺血性脑中风的作用机制研究提供新的思路和实验依据。方法:通过采用线栓法制作大鼠大脑中动脉栓塞模型(MCAO),连续7天对MCAO大鼠内关穴进行针刺,以地机穴作为相对无关穴位进行对照,运用神经行为学评分、缺血脑组织形态学观察、生物化学检测及免疫组织化学检测等方法,通过观察MCAO大鼠神经功能恢复情况,脑组织谷氨酸、谷氨酰胺合成酶的含量,星形胶质细胞GLT1、GS的蛋白表达情况。初步探讨针刺对MCAO大鼠谷氨酸-谷氨酰胺循环关键环节谷氨酸转运体1(GLT1)和谷氨酰胺合成酶(GS)的调控作用。结果:1.连续针刺内关穴7天能够明显改善MCAO大鼠脑组织损伤情况,并促进神经功能的恢复。2.针刺内关穴可以使MCAO大鼠脑组织中过量的谷氨酸减少;并且提高脑组织中的谷氨酰胺合成酶的含量。3.针刺内关穴能够提高星形胶质细胞内GLT1和GS的蛋白表达。结论:针刺内关穴能够通过提高星形胶质细胞GLT1和GS表达,对星形胶质细胞谷氨酸代谢通路进行调控,增强对过多谷氨酸的清除的能力,减轻神经兴奋性毒性,发挥对缺血脑组织的保护和治疗作用。
[Abstract]:Objective: from the astrocyte glutamate metabolism pathway angle, preliminary study of acupuncture on glutamate - glutamine MCAO rats key circulating glutamate transporter 1 (GLT1) and glutamine synthetase (GS) regulation, provide new ideas and experimental basis for research on the mechanism of acupuncture treatment of ischemic stroke. Methods: the rat models of middle cerebral artery occlusion by suture method (MCAO), 7 consecutive days of MCAO rats by acupuncture Neiguan acupoint to acupoint, machine as relatively independent control, using the neurobehavioral score of ischemic brain tissue morphology, biochemical assay and immunohistochemistry method the recovery of nerve function, through the observation of MCAO rats in the brain tissue of glutamic acid, glutamine synthetase, the expression of GLT1 in astrocytes, GS protein. The effects of acupuncture on glutamate transporter 1 (GLT1) and glutamine synthetase (GS) in the key link of glutamate - glutamine cycle in MCAO rats were preliminarily investigated. Results: 1. continuous acupuncture of Neiguan point for 7 days can obviously improve the brain tissue damage in MCAO rats and promote the recovery of nerve function. 2. the acupuncture of Neiguan point can reduce the excess glutamic acid in the brain tissue of MCAO rats and increase the content of glutamine synthetase in brain tissue. 3. acupuncture Neiguan acupoint can improve the protein expression of GLT1 and GS in astrocytes. Conclusion: acupuncture can increase the expression of GLT1 and GS in astrocytes, regulation of astrocytic glutamate metabolism pathway, enhancement of the excessive glutamate scavenging ability, reducing excitotoxicity, protect and treatment of ischemic brain tissue for.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245.3

【参考文献】