针刺曲池与合谷对发热大鼠PGE2和cAMP及IL-1β含量的影响

发布时间：2017-12-30 23:39

本文关键词：针刺曲池与合谷对发热大鼠PGE2和cAMP及IL-1β含量的影响　出处：《湖南中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:通过针刺曲池、合谷穴观察发热大鼠体温变化情况,检测大鼠下丘脑组织中PGE2、c AMP及血浆IL-1β含量,探讨针刺退热效应及其机理。方法:将大鼠随机分成6组,除正常组外,将造模成功的50只大鼠按随机表随机分成模型组(灌服蒸馏水)、中药组(灌服银翘解毒颗粒混悬液)、西药组(灌服阿司匹林混悬液)、单纯针刺组(针刺曲池、合谷)、非经非穴组(针刺曲池、合谷穴旁开0.5cm),每组10只。各组随机抽取5只大鼠在治疗后2h取腹主动脉血及下丘脑,予以待检血浆IL-1β及下丘脑组织中PGE2、c AMP的含量;各组其余的5只大鼠在治疗后1h、2h、3h、4h分别测量体温并记录,并于治疗后4h测量体温后取腹主动脉血及下丘脑,予以待检相应指标。结果:1、对酵母致热大鼠体温的影响西药组、中药组、针刺组在治疗后各个时间点体温均下降,其中西药组、针刺组在治疗后各个时间点体温均低于中药组,针刺组治疗后1h体温低于西药组,差异均有统计学意义(P0.01,P0.05);西药组治疗后2h,针刺组治疗后4h体温与正常组相当,差异无统计学意义(P0.05);非经非穴组体温变化与模型组相当,差异无统计学意义(P0.05)。2、对酵母所致发热大鼠下丘脑组织中PGE2、c AMP及血浆IL-1β含量的影响各组治疗2小时后,针刺组下丘脑组织中PGE2、c AMP、血浆IL-1β分别与模型组相比均有统计学意义(P0.05),与西药组相比均有统计学意义(P0.05),西药组与正常组相比无统计学意义(P0.05);治疗4小时后,针刺组下丘脑组织中PGE2、c AMP、血浆IL-1β分别与模型组、中药组相比均有统计学意义(P0.05),与西药组、正常组相比均无统计学意义(P0.05)。给药后2h、4h各组的体温值分别与相应PGE2、c AMP、IL-1β含量进行相关分析,结果表明二者之间均存在明显正相关(P0.01)。结论:1、针刺组、中药组、西药组均有退热效应,其中西药组、针刺组退热效应优于中药组,针刺退热存在穴位特异性;针刺组退热起效时间优于西药组,西药组优于中药组;西药组解热时间优于针刺组,针刺组优于中药组。2、针刺曲池、合谷退热机制可能主要是通过降低大鼠下丘脑组织中PGE2、c AMP、血浆IL-1β含量而实现的。
[Abstract]:Objective: to observe the changes of body temperature in febrile rats by acupuncture of Quchi and Hegu points, and to detect the content of PGE2C AMP and plasma IL-1 尾 in hypothalamus of rats. Methods: the rats were randomly divided into 6 groups. In addition to the normal group, 50 successful rats were randomly divided into the model group (fed with distilled water) according to the random list. The traditional Chinese medicine group (Yinqiao jiedu granule suspension), the western medicine group (taking aspirin suspension, the simple acupuncture group) (acupuncture qu Chi, Hegu Wu, non-meridian non-point group). 10 rats in each group were randomly selected to collect abdominal aortic blood and hypothalamus 2 hours after treatment, and the plasma IL-1 尾 and the content of PGE2C AMP in hypothalamus were measured. The body temperature of the other 5 rats in each group was measured and recorded at 1h, 2h and 3h after treatment, and the abdominal aorta blood and hypothalamus were taken 4 hours after treatment. Results the body temperature of rats caused by yeast fever was decreased in the western medicine group, traditional Chinese medicine group and acupuncture group at each time point after treatment, among which the western medicine group. The body temperature of the acupuncture group was lower than that of the traditional Chinese medicine group at each time point after treatment, and the body temperature of the acupuncture group was lower than that of the western medicine group at 1 hour after treatment, and the difference was statistically significant. The body temperature in the western medicine group was similar to that in the normal group at 4 hours after treatment, and there was no significant difference between the two groups (P 0.05). The change of body temperature in non-meridian non-point group was similar to that in model group, but there was no significant difference (P 0.05). 2. PGE2 in hypothalamus of febrile rats induced by yeast was observed. The effect of c AMP and plasma IL-1 尾 on PGE2c AMP in hypothalamus of acupuncture group 2 hours after treatment. Compared with the model group, the plasma IL-1 尾 had statistical significance (P 0.05), and compared with the western medicine group, the plasma IL-1 尾 had statistical significance (P 0.05). There was no significant difference between the western medicine group and the normal group (P 0.05). After 4 hours of treatment, PGE2AMP and plasma IL-1 尾 in the hypothalamus of acupuncture group were significantly higher than those in model group and traditional Chinese medicine group. There was no significant difference between the normal group and the control group (P 0.05). The temperature of each group was correlated with the content of IL-1 尾 in the corresponding PGE2C AMPP 4 h after administration. The results showed that there was a significant positive correlation between the two groups (P 0.01). Conclusion the antipyretic effect of the western medicine group and the acupuncture group is better than that of the traditional Chinese medicine group, and the antipyretic effect of the western medicine group and the acupuncture group is better than that of the traditional Chinese medicine group. Acupuncture has acupoint specificity in antipyretic; The antipyretic effect of acupuncture group was better than that of western medicine group, and that of western medicine group was better than that of traditional Chinese medicine group. The antipyretic time of the western medicine group was better than that of the acupuncture group, and the acupuncture group was superior to the traditional Chinese medicine group. The content of plasma IL-1 尾 was achieved.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245

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