基于相对和绝对定量同位素标记技术的脾气虚大鼠回肠蛋白质组学研究

发布时间：2017-12-31 12:25

本文关键词：基于相对和绝对定量同位素标记技术的脾气虚大鼠回肠蛋白质组学研究　出处：《中华中医药杂志》2017年12期 　论文类型：期刊论文

【摘要】：目的:应用相对和绝对定量同位素标记(iTRAQ)技术研究脾气虚大鼠回肠蛋白质组的表达,寻找有意义的差异蛋白,为探索脾气虚证的现代科学本质提供新的实验方法与依据。方法:12只健康雄性SD大鼠随机分为正常组和脾气虚组,脾气虚组采用饮食不节加力竭游泳方法造模,15d后分离提取回肠总蛋白,以iTRAQ试剂标记后进行质谱检测,并对差异表达的蛋白进行生物学分析。结果:与正常组比较,脾气虚组回肠271个上调蛋白,303个下调蛋白;Pathway显著性富集分析确定下调差异蛋白参与的主要生化代谢途径和信号转导途径有细胞外基质受体交互作用、黏着斑等19条,上调差异蛋白参与包括PPAR信号通路、甾类激素生物合成在内的通路24条。选择细胞外基质受体交互作用为兴趣通路,发现与小肠屏障功能密切相关的层粘连蛋白、胶原蛋白以及整合蛋白等19个蛋白发生下调。结论:脾气虚证可能与回肠细胞外基质受体交互作用通路改变及回肠黏膜屏障功能受损相关。
[Abstract]:Objective: to study the expression of proteome in ileum of rats with spleen qi deficiency by using relative and absolute quantitative isotope labeling technique. Methods 12 healthy male SD rats were randomly divided into normal group and spleen qi deficiency group. In the spleen qi deficiency group, the total ileum protein was separated and extracted 15 days later by the method of diet and exhaustive swimming. The total ileum protein was detected by mass spectrometry after labeling with iTRAQ reagent. Results: compared with the normal group, 271 upregulated proteins were upregulated and 303 down-regulated proteins were found in the ileum of spleen Qi deficiency group. Pathway significant enrichment analysis confirmed that the main biochemical metabolic pathways and signal transduction pathways involved in down-regulation of differentially expressed proteins were extracellular matrix receptor interaction and adhesion plaque. Upregulated differential protein involved in 24 pathways including PPAR signaling pathway and steroid hormone biosynthesis. The extracellular matrix receptor interaction was selected as the interest pathway. Laminin was found to be closely related to intestinal barrier function. Conclusion: the deficiency of temper syndrome may be related to the change of extracellular matrix receptor interaction pathway and the impairment of ileal mucosal barrier function.
【作者单位】：辽宁中医药大学中医脏象理论及应用教育部重点实验室;辽宁省中医转化医学研究中心;辽宁中医药大学基础医学院;辽宁中医药大学附属医院;
【基金】：国家重点基础研究发展计划(973计划)项目(No.201133CB531702)~~
【分类号】：R228;R-332
【正文快照】： Proteome research of ileum in spleen qi deficiency rats based on i TRAQ techniqueMA Yi-xin1,2,SONG Nan1,2,JIA Lian-qun1,2,WANG De-shan3,SHAN De-hong3,ZHANG Zhe4,YANG Guan-lin1,2中医理论认为脾为后天之本,气血生化之源。“脾”不仅自身具有重要的生理功能,同时

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