电针联合跑台训练对大鼠骨骼肌钝挫伤的修复作用

发布时间：2018-01-01 08:29

  本文关键词：电针联合跑台训练对大鼠骨骼肌钝挫伤的修复作用　出处：《针刺研究》2017年05期 　论文类型：期刊论文

  更多相关文章： 骨骼肌钝挫伤 电针联合跑台 哺乳动物雷帕霉素靶蛋白 肌细胞生成素 快肌型骨骼肌肌球蛋白重链

【摘要】：目的:观察电针联合跑台训练对大鼠骨骼肌钝挫伤的修复作用,探讨电针联合跑台训练改善骨骼肌损伤的可能机制。方法:SD大鼠随机分为正常组、模型组、电针组、跑台组和联合组,每组12只。采用自制打击装置建立骨骼肌钝挫伤模型。电针组电针"足三里"、阿是穴,跑台组进行跑台训练,联合组同时进行电针和跑台训练,1次/d,连续至损伤后3、14d。HE染色观察损伤后14d腓肠肌新生肌细胞横截面积和直径,Western blot法检测损伤后3d腓肠肌哺乳动物雷帕霉素靶蛋白(mTOR)、肌细胞生成素(myoG)和14d腓肠肌mTOR、快肌型骨骼肌肌球蛋白重链(Fast MyHC)的表达量。结果:模型组新生肌细胞横截面积和直径明显小于正常组(P0.01),各治疗组均显著大于模型组(P0.05),联合组显著大于电针组和跑台组(P0.05)。与正常组比较,损伤后3d模型组腓肠肌mTOR和myoG蛋白表达均显著升高(P0.01);与模型组比较,各治疗组腓肠肌mTOR和myoG蛋白表达均显著升高(P0.01);与电针组比较,跑台组腓肠肌mTOR和myoG蛋白表达均显著降低(P0.01);与电针组和跑台组比较,联合组腓肠肌mTOR和myoG蛋白表达显著升高(P0.05,P0.01)。与正常组比较,损伤后14d模型组腓肠肌mTOR蛋白表达升高,但差异无统计学意义(P0.05),Fast MyHC蛋白表达显著降低(P0.01);与模型组比较,各治疗组腓肠肌mTOR及Fast MyHC蛋白表达均显著升高(P0.05,P0.01);与电针组比较,跑台组腓肠肌mTOR及Fast MyHC蛋白表达均显著降低(P0.05,P0.01);与电针组和跑台组比较,联合组腓肠肌mTOR及Fast MyHC蛋白表达均显著升高(P0.01)。结论:电针联合跑台训练能促进成肌细胞分化成熟,减轻骨骼肌损伤,该作用与增加mTOR表达量,正向调控myoG、Fast MyHC的表达有关。
[Abstract]:Objective: To observe the effect of electroacupuncture combined with rehabilitation training on the run and skeletal muscle contusion of rats, and explore the possible mechanism of electroacupuncture combined with treadmill training improves skeletal muscle injury. Methods: SD rats were randomly divided into normal group, model group, electroacupuncture group, running group and combination group, 12 rats in each group. Using the self-made striking device skeletal muscle contusion model. The electroacupuncture group and Electroacupuncture of "Zusanli", Ashi, treadmill treadmill training group, combination group and electroacupuncture and treadmill training, 1 /d, continuous to staining 14d after injury of gastrocnemius muscle cells of newborn cross-sectional area and the diameter of 3,14d.HE after injury, Western blot detection of 3D after injury of gastrocnemius muscle of the mammalian target of rapamycin (mTOR), myogenin (myoG) and 14d gastrocnemius mTOR, fast twitch skeletal muscle type myosin heavy chain (Fast MyHC) expression. Results: the model group of new muscle cell cross-sectional area and diameter significantly Less than the normal group (P0.01), the treatment group were significantly higher than the model group (P0.05), combination group was significantly higher than that in the electroacupuncture group and treadmill group (P0.05). Compared with the normal group, the expression of 3D after injury in model group gastrocnemius mTOR and myoG protein were significantly increased (P0.01); compared with the model group, the expression of the treatment group gastrocnemius mTOR and myoG protein were significantly increased (P0.01); compared with the electroacupuncture group, running group gastrocnemius mTOR and myoG protein expression were significantly decreased (P0.01); and the electroacupuncture group and treadmill group compared with gastrocnemius mTOR and myoG protein expression was significantly increased (P0.05, P0.01). With the normal group, model group, 14d expression of gastrocnemius muscle mTOR protein increased after injury, but the difference was not statistically significant (P0.05), the expression of Fast MyHC protein was significantly decreased (P0.01); compared with the model group, the expression of each treatment group mTOR and Fast MyHC protein in the gastrocnemius muscle were significantly increased (P0.05, P0.01); compared with the electroacupuncture group run, Taiwan Group mTOR and Fast gastrocnemius muscle MyHC protein expression were significantly decreased (P0.05, P0.01); and the electroacupuncture group and treadmill group compared with group mTOR and Fast gastrocnemius muscle MyHC protein expression increased significantly (P0.01). Conclusion: electroacupuncture combined with treadmill training can promote myoblast differentiation and maturation, reduce the injury of skeletal muscle this effect, with the increase of mTOR expression, the positive regulation of myoG, the expression of Fast MyHC.

【作者单位】： 重庆医科大学中医药学院;
【基金】：国家自然科学基金项目(No.81403466、81273870) 重庆市渝中区项目(No.20140117) 重庆市卫计委项目(No.ZY 20150250) 重庆市教委项目(No.KJ 1702033) 重庆市体育局项目(No.B 201408)
【分类号】：R245
【正文快照】： 急性骨骼肌钝挫伤是骨骼肌运动性损伤中最常见的类型之一[1],以肌纤维的断裂和溶解为主要病变,其病程长且预后差,导致骨骼肌的结构和功能遭到损害,严重影响患者的生活质量,因此骨骼肌损伤后的康复治疗是该领域研究的重点。研究表明建立动物急性骨骼肌钝挫伤模型,对研究骨骼肌

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