探“先天、后天”理论在促进去卵巢小鼠骨质疏松性骨折愈合中的作用

发布时间：2018-01-01 09:01

本文关键词：探“先天、后天”理论在促进去卵巢小鼠骨质疏松性骨折愈合中的作用　出处：《中华中医药杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的:研究补肾、健脾法促进去卵巢小鼠骨质疏松性骨折愈合的作用,探讨中医"先天之本""后天之本"理论在骨质疏松性骨折防治中的运用。方法:建立骨质疏松性骨折小鼠模型,随机分为生理盐水组、健脾组、补肾组,分别给予0.9%氯化钠溶液、健脾方、补肾方灌胃治疗,在术后治疗7、14、28d分别处死小鼠、取材,进行相关指标检测,包括血清雌二醇(E_2)和影像学检测。结果:血清E2检测发现,与生理盐水组比较,补肾组在术后28d可明显提高去卵巢小鼠血清E2含量(P0.05);X-Ray线检测和Micro-CT扫描三维重建结果显示,补肾组和健脾组都能增加骨痂的形成,促进骨质疏松性骨折的愈合;Micro-CT定量分析结果显示,与生理盐水组比较,补肾组7d时骨痂BMD of BV,14d时BV/TV、Conn.D.、BMD of BV、BMD of TV均明显增加(P0.05),健脾组7、14d时骨痂BV/TV、BMD of TV亦明显增加(P0.05)。表明补肾方和健脾方都能提高骨痂的骨量。结论:补"先天"通过雌激素样作用,养"后天"可能通过提供血液濡养和促进药物吸收,加速骨质疏松性骨折愈合。
[Abstract]:Objective: To study the effect of spleen in mouse ovary, promote the healing of osteoporotic fracture, to explore the Chinese medicine "the birth of the" constitution "theory in osteoporotic fracture prevention in use. Methods: to establish a mouse model of osteoporotic fracture, were randomly divided into saline group, Jianpi Bushen group group. Sodium Chloride Solution, were given 0.9%, Jianpi Bushen decoction, gavage treatment, after treatment of 7,14,28d mice were sacrificed, specimens, the related indexes were detected, including serum estradiol (E_2) test and imaging. Results: the serum E2 detection showed that compared with the normal saline group, Bushen group in postoperative 28d to improve the content of E2 in serum of mouse ovary (P0.05); X-Ray line detection and Micro-CT scan showed that the group of tonifying kidney and strengthening spleen group can increase the callus formation, promote the healing of osteoporotic fracture; the results of quantitative analysis of Micro-CT Show that compared with the normal saline group, Bushen group 7d bone BMD of BV, 14d BV/TV, Conn.D. BMD, of BV, BMD of and TV were significantly increased (P0.05), 7,14d group of invigorating spleen and bone callus BV/TV, BMD of TV also increased significantly (P0.05). That can Jianpi Bushen recipe and to improve the callus bone. Conclusion: the prescription of "innate" by estrogen like effects, have acquired through blood Ruyang and promote drug absorption, accelerate the healing of osteoporotic fracture.

【作者单位】：上海中医药大学附属龙华医院;上海中医药大学脊柱病研究所;
【基金】：国家自然科学基金青年基金项目(No.81473701) 上海市科委青年科技启明星计划项目(No.14QA1403500);上海市科委中医重点项目(No.15401971700) 上海市卫生系统新优青培养计划项目(No.XYQ2013085) 上海市教育委员会科研创新项目(No.14YZ051)~~
【分类号】：R274.1
【正文快照】： 随着现代医学的发展,绝经后妇女骨质疏松症的防治受到越来越多的关注。绝经后骨质疏松症(postmenopausalosteoporosis,PMOP)是指发生在绝经后妇女中的一种以全身性单位体积内骨组织含量减少,骨密度(bone mineral density,BMD)下降及骨组织微结构退行性病变为特征的全身性骨骼

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