寻常型银屑病不同证型蛋白组学表达的比较研究

发布时间：2018-01-02 04:00

  本文关键词：寻常型银屑病不同证型蛋白组学表达的比较研究　出处：《湖南中医药大学学报》2017年03期 　论文类型：期刊论文

  更多相关文章： 寻常型银屑病 血热证 血瘀证 血虚证 双向电泳 蛋白质组学 差异蛋白

【摘要】：目的对寻常型银屑病(血热证、血瘀证、血虚证)患者与正常人差异蛋白进行分析,探求三证之间差异性表达蛋白,从蛋白质组学角度揭示三证之间的差异。方法取寻常型银屑病血瘀证、血虚证、血热证患者的血清,应用蛋白提取、定量、双向电泳技术、质谱鉴定和数据库检索,寻找出三证之间的差异蛋白和共有蛋白,并将这些蛋白进行组间比较。结果发现了15种显著性差异蛋白(P0.05),其中补体C3、凝聚素、血清转铁蛋白、间-α-胰蛋白酶抑制剂H4重链(片段)、载脂蛋白E、补体C4-B、甘露糖结合蛋白C及富亮氨酸-α-2-糖蛋白在血虚型银屑病患者血清表达水平高于血热型和血瘀型(P0.05)。纤维蛋白原β链、血液接合素、纤维蛋白原γ链、HPX蛋白、α-抗胰蛋白酶、甲状腺素转运蛋白及载脂蛋白Ⅳ在血热型银屑病患者血清中的表达水平高于血瘀型患者(P0.05)。共同蛋白有5种(P0.05),分别为血液结合素、纤维蛋白原γ链、HPX蛋白、α-抗胰蛋白酶、载脂蛋白A-Ⅳ。结论寻常型银屑病(血热证、血瘀证、血虚证)血清中存在表达差异性蛋白质,可能与寻常型银屑病的发病机制和银屑病不同证型的物质基础有关。
[Abstract]:Objective to analyze the differential protein between patients with psoriasis vulgaris (blood heat syndrome, blood stasis syndrome, blood deficiency syndrome) and normal people, and to explore the differential expression of protein between the three syndromes. Methods Blood stasis syndrome, blood deficiency syndrome and blood heat syndrome of psoriasis vulgaris were collected from the serum of patients with blood stasis syndrome, blood deficiency syndrome and blood heat syndrome, and protein extraction, quantitative analysis and two dimensional electrophoresis were used to detect the differences between the three syndromes from the perspective of proteomics. The differential proteins and common proteins among the three syndromes were identified by mass spectrometry and database retrieval. The results showed that there were 15 significant differential proteins (P0.05). Among them, complement C3, agglutinin, serum transferrin, m-伪 -trypsin inhibitor H4 heavy chain (fragment, apolipoprotein E, complement C4-B). The expression level of mannose binding protein C and leucine-rich 伪 -2-glycoprotein in serum of psoriasis patients with blood deficiency was higher than that of P0.05, fibrinogen 尾 chain and blood conjugate in psoriatic patients with blood deficiency. Fibrinogen 纬 chain HPX protein, 伪 -antitrypsin. The expression of thyroxine transporter and apolipoprotein 鈪，

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