基于“健脾益气”法探讨针刺脾俞穴联合人参皂苷Rg3抗疲劳机制的实验研究

发布时间：2018-01-02 07:03

本文关键词：基于“健脾益气”法探讨针刺脾俞穴联合人参皂苷Rg3抗疲劳机制的实验研究　出处：《辽宁中医药大学》2016年博士论文　论文类型：学位论文

【摘要】：目的:本课题采用高效液相色谱法观察针刺“脾俞”穴对人参皂苷Rg3在慢性疲劳模型大鼠不同时相脏腑的分布代谢影响,采用光学显微镜观察大鼠血清中T淋巴细胞转化率,流式细胞术检测大鼠脾脏中T细胞亚群CD_3~+T细胞、CD_4~+T细胞及CD_8~+T细胞比例,CD_4~+/CD_8~+的比值,ELISA法检测血清中IFN-γ和IL-4含量变化,RT-PCR法检测脾脏中IFN-γ和IL-4 m RNA表达水平,采用Western Blot法检测脾脏中IFN-γ和IL-4蛋白表达水平,探讨针刺“脾俞”穴联合人参皂苷Rg3,“针药结合”益气健脾抗疲劳的作用机理。材料与方法:论文一:雄性Wistar大鼠116只随机分为4组:空白对照组(33只),模型组(33只),空白加针刺组(25只),模型加针刺组(25只)。采用强制冷水游泳结合慢性束缚法进行模型复制。造模时间21d。21d后,模型组和空白对照组大鼠各随机抽取8只大鼠,通过检测其在造模前后的体重和血清乳酸含量的改变情况来判定造模是否成功。空白加针刺组和模型加针刺组于模型复制成功后,给予针刺“脾俞”穴治疗,进针后针柄接电针治疗仪,电压4.5 V,频率为4/20 Hz频率的疏密波,强度以大鼠局部皮肤肌肉微颤为度,留针20 min,1次/d,第7天针刺结束后,各组大鼠立即尾静脉注射人参皂苷Rg3。各组大鼠分别取5只,于给药后5、30、60、120 min经大鼠眼眶取血2ml于肝素化离心管中,3000 rpm离心10 min,分离血浆,高效液相色谱法检测各时间段各组大鼠血浆中人参皂苷Rg3的含量。各组剩余的20只大鼠分别于0.5、1、2、4 h各处死5只,采集心、肝、脾、肺和肾组织,取组织样品0.5 g,加入甲醇1 ml,组织匀浆,震荡10 min,超声10 min,4000rpm离心10 min,收集上清液,高效液相色谱法检测各时间段各组大鼠心、肝、脾、肺和肾中人参皂苷Rg3的含量。论文二:雄性Wistar大鼠40只,随机分为空白对照组,模型组,人参皂苷组,针刺组,人参皂苷联合针刺组,每组8只。采用强制冷水游泳结合慢性束缚法进行模型复制。模型复制成功后,针刺组给予针刺“脾俞”穴治疗,人参皂苷组给予大鼠尾静脉注射人参皂苷Rg3,人参皂苷联合针刺组,先给大鼠尾静脉注射人参皂苷Rg3,再给予针刺“脾俞”穴,1次/d,连续治疗7天。末次给药及针刺后1h,各组大鼠断尾取血,制作血涂片,采用光学显微镜观察大鼠血清中T淋巴细胞转化率;腹主动脉取血后剪开腹腔摘取脾脏,制作脾细胞悬液,用流式细胞仪检测大鼠脾脏中T细胞亚群CD_3~+T细胞、CD_4~+T细胞和CD_8~+T细胞的比例,及CD_4~+T/CD_8~+T的比值。论文三:雄性Wistar大鼠40只随机分为空白对照组,模型组,人参皂苷组,针刺组,人参皂苷联合针刺组,每组8只。各组大鼠模型复制及干预结束后,用10%水合氯醛以3ml/kg体质量腹腔注射麻醉,腹主动脉穿刺采集全血,取上清液冻存于-80℃冰箱中,Elisa法检测血清中IFN-γ和IL-4含量。同时采集大鼠脾脏组织,采用RT-PCR法检测脾脏中IFN-γ和IL-4 m RNA表达水平,Western Blot法检测脾脏中IFN-γ和IL-4蛋白表达水平。结果:论文一:1模型评价:造模前两组大鼠体重差异无统计学意义(p0.05),造模后与空白对照组比较,模型组体重显著减少(p0.01),乳酸含量显著升高(p0.01),说明模型复制成功。2各组大鼠血浆中人参皂苷Rg3含量:与空白对照组同期比较,模型组60min及120min血浆中人参皂苷Rg3的含量显著降低,空白针刺组60min及120min血浆中人参皂苷Rg3的含量显著增加(P0.05);与模型组比较,模型针刺组及空白针刺组60min及120min血浆中人参皂苷Rg3的含量显著增加(P0.05)。在5 min时血浆中人参皂苷Rg3的含量最高,之后逐渐降低。3各组大鼠肝脏中人参皂苷Rg3分布:与空白对照组同期比较,模型组1 h、2 h及4 h人参皂苷Rg3的含量显著降低(P0.05),空白针刺组1 h、2 h及4 h人参皂苷Rg3的含量显著增加(P0.05),模型针刺组无明显变化;与模型组比较,模型针刺组及空白针刺组1 h、2 h及4 h人参皂苷Rg3的含量均显著增加(P0.05)。1 h时肝脏中人参皂苷Rg3的含量达到峰值,之后逐渐降低。4各组大鼠心脏中人参皂苷Rg3分布:与空白对照组同期比较,模型组0.5 h及1 h人参皂苷Rg3的含量显著降低(P0.05),空白针刺组0.5 h及1 h人参皂苷Rg3的含量显著增加(P0.05);与模型组比较,模型针刺组及空白针刺组0.5 h及1 h人参皂苷Rg3的含量均显著增加(P0.05)。心脏中0.5 h时人参皂苷Rg3的含量最高,之后逐渐降低,四组大鼠心脏组织中4 h时均检测不到人参皂苷Rg3。5各组大鼠脾脏中人参皂苷Rg3分布:与空白对照组比较,模型组0.5h、1 h、及2h人参皂苷Rg3的含量显著降低(P0.05),空白针刺组0.5 h、1 h、及2 h人参皂苷Rg3的含量显著增加(P0.05);与模型组比较,模型针刺组及空白针刺组0.5h、1 h、及2 h人参皂苷Rg3的含量均显著增加(P0.05)。脾脏中0.5 h时人参皂苷Rg3的含量最高,之后逐渐降低。6各组大鼠肺脏中人参皂苷Rg3分布:与空白对照组同期比较,模型组各时间点人参皂苷Rg3的含量均降低,空白针刺组各时间点人参皂苷Rg3的含量均升高,但均不具有统计学意义(P0.05);与模型组比较,空白针刺组1 h、2 h人参皂苷Rg3的含量显著增加(P0.05),模型针刺组各时间点人参皂苷Rg3的含量均有增加,但不具有统计学意义(P0.05),四组大鼠肺脏组织中4 h时均检测不到人参皂苷Rg3。7各组大鼠肾脏中人参皂苷Rg3分布:与空白对照组同期比较,模型组2 h及4 h人参皂苷Rg3的含量显著降低(P0.05),空白针刺组2 h及4 h人参皂苷Rg3的含量显著增加(P0.05);与模型组比较,模型针刺组及空白针刺组2 h及4 h人参皂苷Rg3的含量均显著增加(P0.05)。肾脏中1 h时人参皂苷Rg3的含量达到峰值,之后逐渐降低。论文二:1各组大鼠淋巴细胞转化率结果:与空白对照组比较,各组大鼠淋巴细胞转化率显著降低(P0.01);与模型组比较,人参皂苷组,针刺组及人参皂苷联合针刺组淋巴细胞转化率显著升高(P0.01);与人参皂苷联合针刺组比较,人参皂苷组和针刺组淋巴细胞转化率显著降低(P0.05);而人参皂苷组与针刺组之间比较无统计学意义(P0.05)。未转化的淋巴细胞呈正圆形,细胞核呈紫色,正圆形;胞浆呈淡蓝色极少,呈新月形位于细胞边缘。发生转化的淋巴母细胞个体增大,细胞核变大,偶见核仁出现,形态不规则,胞浆明显增多,胞浆内偶见空泡出现。2流式细胞结果:CD_3~+T细胞比例结果:与空白对照组比较,模型组CD_3~+T细胞比例显著降低(P0.01),人参皂苷组CD_3~+T细胞比例显著降低(P0.05);与模型组比较,针刺组CD_3~+T细胞比例显著升高(P0.01),人参皂苷联合针刺组CD_3~+T细胞比例显著升高(P0.05);而人参皂苷组与针刺组之间比较无统计学意义(P0.05)。CD_4~+T细胞比例结果:与空白对照组比较,模型组CD_4~+T细胞比例升高,差异有统计学意义(P0.05);与模型组比较,人参皂苷联合针刺组CD_4~+T细胞比例降低,差异有统计学意义(P0.05)。CD_8~+T细胞比例结果:与空白对照组比较,各组大鼠CD_8~+T细胞比例显著降低(P0.01);与模型组比较,针刺组、人参皂苷组、人参皂苷联合针刺组CD_8~+T细胞比例均显著升高(P0.01);与人参皂苷联合针刺组比较,人参皂苷组CD_8~+T细胞比例显著降低(P0.01)。CD_4~+T/CD_8~+T结果:与空白对照组比较,模型组、皂苷组CD_4~+T/CD_8~+T比值均显著升高(P0.01);与模型组比较,针刺组、人参皂苷组及人参皂苷联合针刺组CD_4~+T/CD_8~+T比值显著降低(P0.01)。与人参皂苷联合针刺组比较,人参皂苷组CD_4~+T/CD_8~+T比值显著升高,(P0.05)。论文三:1各组大鼠血清中IFN-γ和IL-4含量结果:与空白对照组比较,各组大鼠血清中IFN-γ含量显著降低(P0.01);与模型组比较,针刺组、皂苷组、人参皂苷联合针刺组IFN-γ含量显著升高(P0.01);与人参皂苷联合针刺组比较,针刺组、人参皂苷组IFN-γ含量显著降低(P0.01);而人参皂苷组与针刺组之间比较无统计学意义(P0.05)。与空白对照组比较,各组大鼠血清中IL-4显著升高(P0.01);与模型组比较,针刺组、皂苷组、人参皂苷联合针刺组IL-4含量显著降低(P0.01);与人参皂苷联合针刺组比较,针刺组、人参皂苷组IL-4含量显著升高(P0.01);而人参皂苷组与针刺组之间比较无统计学意义(P0.05)。2各组大鼠脾脏中IFN-γ和IL-4的蛋白表达水平结果:与空白对照组比较,各组大鼠IFN-γ蛋白表达水平显著降低(P0.01);与模型组比较,针刺组、皂苷组、人参皂苷联合针刺组IFN-γ蛋白表达水平显著升高(P0.01);与人参皂苷联合针刺组比较,针刺组和人参皂苷组IFN-γ蛋白表达水平显著降低(P0.05);而人参皂苷组与针刺组之间比较无统计学意义(P0.05)。与空白对照组比较,各组大鼠IL-4蛋白表达水平显著升高(P0.01);与模型组比较,针刺组、皂苷组、人参皂苷联合针刺组IL-4蛋白表达显著水平降低(P0.01);与人参皂苷联合针刺组比较,针刺组和人参皂苷组IL-4蛋白表达水平显著降低(P0.05);而人参皂苷组与针刺组之间比较无统计学意义(P0.05)。3各组大鼠脾脏中IFN-γ和IL-4 m RNA表达水平结果:与空白对照组比较,各组大鼠IFN-γm RNA表达水平显著降低(P0.01);与模型组比较,针刺组、皂苷组、人参皂苷联合针刺组IFN-γm RNA表达水平显著升高(P0.01);与人参皂苷联合针刺组比较,针刺组和人参皂苷组IFN-γm RNA表达水平显著降低(P0.05);而人参皂苷组与针刺组之间比较无统计学意义(P0.05)。与空白对照组比较,各组大鼠IL-4 m RNA表达水平显著升高(P0.01);与模型组比较,针刺组、皂苷组、人参皂苷联合针刺组IL-4 m RNA表达水平显著降低(P0.01);与人参皂苷联合针刺组比较,针刺组和人参皂苷组IL-4 m RNA表达水平显著升高(P0.05);而人参皂苷组与针刺组之间比较无统计学意义(P0.05)。结论:1.针刺“脾俞”穴可以增加人参皂苷Rg3在血内的含量和在五脏中的分布,延长药物在体内的停留时间,这可能是针药并用协同增效的物质基础。2.针刺“脾俞”穴和静脉注射人参皂苷Rg3是通过纠正慢性疲劳模型大鼠Th1/Th2失衡状态,调节T淋巴细胞亚群,增强机体细胞免疫功能实现其抗疲劳作用。3.针刺“脾俞”穴和静脉注射人参皂苷Rg3均具有抗疲劳作用,并且二者联用具有协同增效作用,为针药并用协同增效抗疲劳提供实验理论基础。
[Abstract]:Objective: This study by high performance liquid chromatography method was used to observe the effect of acupuncture points on the distribution and metabolism of spleen Shu "of ginsenoside Rg3 in chronic fatigue rat model and observe the viscera, T lymphocyte transformation rate in rat serum by optical microscopy, rat spleen cells were detected by flow cytometry in T cell subsets of CD_3~+T cells, CD_4~+T cells and CD_8~+T cell ratio, CD_4~+/CD_8~+ ratio, IFN- content changes of gamma and IL-4 in serum were measured by ELISA, RT-PCR in spleen was detected in IFN- gamma and IL-4 m expression level of RNA, using Western Blot method to detect spleen IFN- gamma and IL-4 protein expression, to investigate the effects of Acupuncture on Pishu points combined with ginsenoside Rg3 the combination of acupuncture and medicine," the mechanism of anti fatigue yiqijianpi. Materials and methods: Part One: 116 male Wistar rats were randomly divided into 4 groups: control group (33 rats), model group (33 rats), the blank plus acupuncture group (25 Only), model plus acupuncture group (25 rats). The forced cold water swimming with chronic restraint method for model replication. The modeling time 21d.21d, model group and blank control group rats were randomly selected 8 rats by detecting the rats in body weight and serum lactate content changes to determine the success of modeling. The blank group and model plus acupuncture plus acupuncture group in the model was established successfully after treated by acupuncture "Pishu" acupuncture treatment, the needle after the needle handle electric acupuncture apparatus, voltage 4.5 V, frequency 4/20 Hz dilatational wave frequency, intensity of local skin and muscle to rats of tremor the needle, 20 min, 1 /d, seventh days after acupuncture treatment, rats were intravenously injected with ginsenoside Rg3. rats were 5 rats after administration of 5,30,60120 min by rat orbital blood 2ml in heparinized centrifuge tubes, 3000 rpm centrifugation 10 min, plasma separation, HPLC color The spectral content of plasma was detected in different time of rats in ginsenoside Rg3. 20 rats in each group were 0.5,1,2,4 h in the remaining 5 rats of each group were sacrificed to collect heart, liver, spleen, lung, and renal tissue, the tissue samples of 0.5 g, add 1 ml of methanol, homogenate, shock 10 min, ultrasound 10 min 4000rpm centrifugation for 10 min, the supernatants were detected in different time of rats heart, HPLC liver, spleen, lung and kidney in the content of ginsenoside Rg3. The two: 40 male Wistar rats were randomly divided into control group, model group, ginsenoside group, needle ginsenosides combined with acupuncture group, acupuncture group, 8 rats in each group. The forced cold water swimming with chronic restraint method for model replication. When the model was established successfully, the acupuncture group was given acupuncture "Pishu" acupuncture therapy, ginsenoside group rats were given intravenous injection of ginsenoside Rg3, ginsenoside combined with acupuncture group, give rats teleutostatic Intravenous injection of ginsenoside Rg3, then treated by acupuncture Pishu points, 1 times /d, for 7 consecutive days. The last drug and acupuncture on 1H rats, tail blood, making blood smears were observed by optical microscopy in rat serum T lymphocyte transformation rate; blood from the abdominal aorta after cut the removal of abdominal spleen, make spleen cell suspension were detected by using flow cytometry in rat spleen T cell subsets of CD_3~+T cells, CD_4~+T cells and CD_8~+T cell ratio and CD_4~+T/CD_8~+T ratio. The three: 40 male Wistar rats were randomly divided into control group, model group, ginsenoside group, acupuncture ginsenoside group, acupuncture group, 8 rats in each group. The rats model of replication and after intervention with 10% chloral hydrate with 3ml/kg body weight intraperitoneal injection of anesthesia, abdominal aortic blood sampling puncture, the supernatant was frozen in -80 C refrigerator, Elisa were detected by IFN- and I The content of L-4. At the same time from spleen tissues of rats was detected by RT-PCR in the spleen of IFN- gamma and IL-4 m RNA expression level of Western in spleen was detected by Blot IFN- gamma and IL-4 protein expression. Results: Part One: 1 model evaluation: before modeling the two groups of rats had no significant difference (P0.05) after modeling, compared with the control group, the weight of the model group decreased significantly (P0.01), lactic acid content increased significantly (P0.01), the model successfully copied the plasma.2 of rats in ginsenoside Rg3 content: compared with the blank control group, model group were 60min and 120min in plasma of ginsenoside Rg3 decreased significantly. The content of 60min in the acupuncture group and blank plasma 120min in ginsenoside Rg3 increased significantly (P0.05); compared with the model group, the content of the model group and the blank acupuncture acupuncture group of 60min and 120min in plasma of ginsenoside Rg3 increased significantly (P0.05). At 5 min in human plasma Ginsenoside Rg3 was the highest, then gradually reduce the distribution of ginsenoside Rg3.3 in liver of rats: compared with the blank control group, model group, 1 h, 2 h and 4 h the content of ginsenoside Rg3 decreased significantly (P0.05), blank acupuncture group 1 h, 2 h and 4 h the content of Ginsenoside Rg3 increased significantly (P0.05) model, the acupuncture group had no obvious change; compared with the model group, model group and blank acupuncture acupuncture group of 1 h, 2 h and 4 h the content of ginsenoside Rg3 significantly increased the content of.1 (P0.05) h in the liver of ginsenoside Rg3 reached the peak, then gradually decreased distribution of Rg3 ginsenoside.4 heart of rats in each group: compared with the blank control group, model group was 0.5 h and 1 h of ginsenoside Rg3 decreased significantly (P0.05), the content of blank acupuncture group of 0.5 h and 1 h of ginsenoside Rg3 increased significantly (P0.05); compared with the model group, model group and blank acupuncture acupuncture group of 0.5 h and 1 h The content of ginsenoside Rg3 increased significantly (P0.05). In the heart of 0.5 h of ginsenoside Rg3 content is the highest, then gradually decreased, the heart tissue in rats of the four groups in 4 h were not detected in the distribution of ginsenoside Rg3 spleen of ginsenoside Rg3.5 in rats: compared with the blank control group, 0.5h model group 1 h, 2h and content of ginsenoside Rg3 decreased significantly (P0.05), blank acupuncture group 0.5 h, 1 h, 2 h and the content of ginsenoside Rg3 increased significantly (P0.05); compared with the model group, acupuncture group, acupuncture group and blank model 0.5h, 1 h, 2 h and the content of ginsenoside Rg3 the increased significantly (P0.05). In the spleen of 0.5 h ginsenoside Rg3 content is the highest, then gradually reduce the distribution of Rg3 ginsenoside.6 in the lung of rats in each group: compared with the blank control group, model group in each time point of ginsenoside Rg3 decreased, the content of blank acupuncture group at each time point of ginsenoside Rg3 the Both increased, but were not statistically significant (P0.05); compared with the model group, blank acupuncture group 1 h, 2 h the content of ginsenoside Rg3 increased significantly (P0.05), were content model acupuncture group at different time points of ginsenoside Rg3 increased, but not statistically significant (P0.05), four groups of rats the lung tissue of 4 h were not detected in the distribution of ginsenoside Rg3 in kidney of ginsenoside Rg3.7 in rats of each group: compared with the blank control group, model group was 2 h and 4 h of ginsenoside Rg3 decreased significantly (P0.05), the content of blank acupuncture group of 2 h and 4 h of ginsenoside Rg3 increased significantly (P0.05); compared with the model group, the content of the model group and the blank acupuncture acupuncture group of 2 h and 4 h of ginsenoside Rg3 were significantly increased (P0.05) in the kidney of 1 h. The content of ginsenoside Rg3 reached the peak, then decreased gradually. The two rats in each group: 1 lymphoid cell conversion rate results: compared with the blank the control group Comparison of lymphocytes of rats significantly decreased the conversion rate (P0.01); compared with the model group, ginsenoside group, acupuncture group and acupuncture group of ginsenosides combined with the lymphocyte transformation rate was significantly increased (P0.01); compared with ginsenosides combined with acupuncture group, ginsenoside group and acupuncture group significantly decreased the rate of lymphocyte transformation (P0.05); between ginsenoside group and acupuncture group was not statistically significant (P0.05). Positive lymphocytes unconverted round nuclei were round, purple, pale blue cytoplasm; rarely, crescent shaped at the edge of the cells. The transformed lymphoblastoid cell nucleus became large, individual increases, occasionally nucleolus, morphology the rules, the cytoplasm increased significantly, cytoplasmic vacuolization occasionally.2 flow cytometry. Results: the percentage of CD_3~+T cells results: compared with the blank control group, model group of CD_3~+T cells was significantly lower than patients (P0.01), ginsenoside CD_3 group The proportion of ~+T cells decreased significantly (P0.05); compared with the model group, the acupuncture group was significantly increased CD_3~+T cell ratio (P0.01), ginsenosides combined with acupuncture group the percentage of CD_3~+T cells increased significantly (P0.05); and between ginsenoside group and acupuncture group had no significant difference between the percentage of.CD_4~+T cells (P0.05) results: compared with the control group group, the proportion of CD_4~+T cell model increased, the difference was statistically significant (P0.05); compared with the model group, the acupuncture group of ginsenosides combined with CD_4~+T were decreased, the difference was statistically significant (P0.05.CD_8) ~+T cell ratio results: compared with the control group, the proportion of CD_8~+T cells of each group rats were significantly decreased (P0.01); comparison with the model group, acupuncture group, ginsenoside group, acupuncture group the proportion of CD_8~+T cells combined with ginsenoside were significantly increased (P0.01); compared with ginsenosides combined with acupuncture group, ginsenoside group the proportion of CD_8~+T cells significantly Reduce (P0.01).CD_4~+T/CD_8~+T results: compared to the control group, model group and blank group, CD_4~+T/CD_8~+T saponins were significantly higher (P0.01); compared with the model group, acupuncture group, ginsenoside group and ginsenoside CD_4~+T/CD_8~+T combined with acupuncture group was significantly lower (P0.01). Compared with the ginsenosides combined with acupuncture group, ginsenoside group the ratio of CD_4~+T/CD_8~+T increased significantly (P0.05). The three: 1 in serum IFN- gamma and IL-4 content results: compared with the control group, IFN- content in serum of rats were significantly decreased (P0.01); compared with the model group, acupuncture group, saponin group, ginsenoside IFN- combined with acupuncture group gamma content increased significantly (P0.01); compared with ginsenosides combined with acupuncture group, acupuncture group, ginsenoside IFN- group gamma content decreased significantly (P0.01); and between ginsenoside group and acupuncture group had no statistical significance (P0.05) and blank control. Group, IL-4 in serum of rats were significantly increased (P0.01); compared with the model group, acupuncture group, saponin group, ginsenoside IL-4 combined with acupuncture group was significantly lower (P0.01); compared with ginsenosides combined with acupuncture group, acupuncture group, ginsenoside IL-4 group was significantly increased (P0.01); and between ginseng the saponin group and acupuncture group had no statistical significance (P0.05) expression of IFN- gamma and IL-4.2 in spleen of rats in protein level. Results: compared with the control group, the expression level of IFN- protein gamma groups rats were significantly decreased (P0.01); compared with the model group, acupuncture group, saponin group, the expression level of ginsenosides combined with the acupuncture group IFN- gamma protein increased significantly (P0.01); compared with ginsenosides combined with acupuncture group, the expression level of acupuncture group and ginsenoside group IFN- gamma protein decreased significantly (P0.05); and ginsenoside group and acupuncture group were no statistical significance (P0.05) and empty. White compared to the control group, the expression level of IL-4 protein in rats were significantly increased (P0.01); compared with the model group, acupuncture group, acupuncture group saponin group, expression of IL-4 protein significantly decreased levels of ginsenoside (P0.01); compared with ginsenosides combined with acupuncture group, acupuncture group and ginsenoside group the expression level of IL-4 protein significantly reduce (P0.05); and between ginsenoside group and acupuncture group was not statistically significant (P0.05) level and IL-4 m results IFN- gamma RNA expression in spleen of.3 rats compared with control group, the expression of IFN- gamma m rats RNA levels decreased significantly (P0.01); compared with the model group, acupuncture group, saponin group, ginsenoside IFN- combined with acupuncture group gamma m RNA expression level was significantly increased (P0.01); compared with ginsenosides combined with acupuncture group, the expression level of acupuncture group and ginsenoside IFN- gamma m RNA group decreased significantly (P0.05); and ginsenoside group and acupuncture group. There was no significant difference (P0.05). Compared with the control group, the expression level of IL-4 m in RNA rats increased significantly (P0.01); compared with the model group, acupuncture group, saponin group, the expression level of ginsenosides combined with acupuncture group IL-4 m RNA decreased significantly (P0.01); compared with ginsenoside combined with acupuncture group. The expression level of acupuncture group and ginsenoside IL-4 group M RNA were significantly increased (P0.05); and between ginsenoside group and acupuncture groups were not statistically significant (P0.05). Conclusion: the contents of 1. acupuncture Pishu points can increase the ginsenoside Rg3 in the blood and in the internal organs in the distribution, prolong the retention time of medicine in in vivo, which may

【学位授予单位】：辽宁中医药大学
【学位级别】：博士
【学位授予年份】：2016
【分类号】：R245

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