电针与敷贴干预高血压相关血管性痴呆大鼠模型的差异性研究

发布时间：2018-01-03 19:31

本文关键词：电针与敷贴干预高血压相关血管性痴呆大鼠模型的差异性研究　出处：《成都中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的：此课题以高血压相关血管性痴呆模型大鼠为研究对象,比较电针与穴位敷贴疗法在治疗前后模型大鼠血压、认知功能及血管内皮生长因子(vascular endothelial growth factor, VEGF)的差异性,为敷贴疗法干预高血压从而防治血管性痴呆提供更为充分的科学依据。方法：本研究以Wistar大鼠为研究对象,通过高盐饮食诱导法与改良2-血管阻断法制备高血压相关血管性痴呆模型,采用鼠尾动脉血压测量与Morris水迷宫进行模型的筛选。将筛选出的模型大鼠随机分为空白组、模型组、电针组和敷贴组,治疗组分别予以电针治疗与敷贴治疗,并在治疗前期、中期与后期对三组大鼠予以鼠尾动脉血压的测量,运用Morris水迷宫检测大鼠的认知及学习记忆能力,并在治疗后采用酶联免疫分析(enzyme-linked immunosorbent assay, ELISA)技术分析各组大鼠血管内皮生长因子(VEGF)的差异变化。结果：1、空白组与其他三组比较,模型制备后其他三组的血压、学习记忆能力及VEGF均具有统计学意义(P0.05)。2、血压电针组与模型组相比,电针治疗后血压有明显降低(P0.05),且治疗时间越长,对大鼠的血压影响越大；敷贴组与模型组相比,敷贴治疗后血压有明显降低(P0.05),且治疗时间越长,对大鼠的血压影响越大。时间因素影响血压值的变化,在不同时点上其差异有统计学意义(P0.05)；在不同的分组和时间之间,血压值均与其有交互作用(P0.05),即时间因素的作用随着分组的不同而存在差异。3、学习记忆能力定位航行试验：电针组与模型组相比,电针治疗之后大鼠的逃避潜伏期显著缩短,其差异有统计学意义(P0.05)；敷贴组与模型组相比,敷贴治疗之后大鼠逃避潜伏期变化不大,差异无明显统计学意义(P0.05)。时间因素影响逃避潜伏期的变化,在不同时点上其差异有统计学意义(P0.05)；在不同的分组和时间之间,逃避潜伏期均与其有交互作用(P0.05),即时间因素的作用随着分组的不同而存在差异。空间探索试验：电针组与模型组相比,电针治疗之后大鼠跨越原平台次数显著增多,其差异有统计学意义(P0.01)；敷贴组与模型组相比,敷贴治疗之后大鼠跨越原平台次数变化不大,差异无明显统计学意义(P0.05)。时间因素影响跨越平台次数的变化,在不同时点上其差异有统计学意义(P0.05)；在不同的分组和时间之间,跨越平台次数均与其有交互作用(P0.05),即时间因素的作用随着分组的不同而存在差异。4、酶联免疫分析试验电针组与模型组相比,电针治疗后VEGF浓度显著增高,两组效果差异有显著统计学意义(P0.01)；敷贴组与模型组相比,敷贴治疗后VEGF有所提高,但效果差异无统计学意义(P0.05)；电针组与敷贴组相比,电针治疗后VEGF浓度显著增高,两组效果差异有显著统计学意义(P0.01)。结论：1、电针与敷贴治疗均可以降低血压,治疗时间越长,降压的功效越明显,且两种疗法的降压疗效相当。2、在行为学测试中,经过一定时间的治疗,电针组大鼠的学习记忆能力相较于模型组有很大的改善,敷贴组大鼠在学习记忆功能的上略有改善,但是作用并不特别明显。3、在酶联免疫分析试验中,通过一定时间的治疗,电针组VEGF浓度提升较为明显,敷贴组VEGF浓度也有一定的提升,说明二种疗法都对大鼠的血管新生及神经保护有一定作用。
[Abstract]:Objective: this subject to hypertension related vascular dementia rats as the research object, comparison of electroacupuncture and acupoint application therapy before and after the treatment in the rat model of blood pressure, cognitive function and vascular endothelial growth factor (vascular endothelial, growth factor, VEGF) the difference for plaster therapy and intervention for hypertension prevention and treatment of vascular dementia with more in order to make scientific basis. Methods: in this study, Wistar rats as the research object, method and modified 2- induced vascular occlusion by hypertension related vascular dementia model by high salt diet, the screening of rat tail artery blood pressure measurement and Morris water maze model. The model rats were selected randomly divided into blank group, model group, electroacupuncture group and acupoint group, treatment group were given acupuncture treatment and sticking therapy, and in the treatment of early, middle and late to tail arterial blood pressure of rats in three groups The measurement, using the cognitive abilities of learning and memory and Morris water maze test in rats, and analyzed by ELISA after treatment (enzyme-linked immunosorbent assay ELISA) technology were analyzed for vascular endothelial growth factor in rats (VEGF). The difference of results: 1, the blank group compared with the other three groups, blood pressure the three groups after model preparation, significant learning and memory ability and VEGF (P0.05).2, the blood pressure in EA group compared with model group, the blood pressure decreased significantly after treatment (P0.05), and the longer the treatment, the blood pressure of rats increased; acupoint group compared with model group sticking the blood pressure was significantly decreased (P0.05), and the longer the treatment, the blood pressure of rats. The greater the time factors influencing blood pressure values in different time points, the difference was statistically significant (P0.05); between group and different time, Blood pressure and their interaction (P0.05), time factor with the grouping of different.3 memory location learning navigation test: EA group compared with model group, electroacupuncture treatment after the rats escape latency was significantly shortened, the difference was statistically significant (P0.05); acupoint group compared with the model group, sticking treatment after the escape latency changes little, the difference was not statistically significant (P0.05). The changes of time factors influencing the escape latency in different time points, the difference was statistically significant (P0.05); in between packet and different time, the escape latency and their interaction (P0.05), time factor effect exists with the group differences. Space exploration test: EA group compared with model group, electroacupuncture treatment of rats after crossing platform times increased significantly, there was statistical difference The significance (P0.01); acupoint group compared with model group, sticking treatment rats crossing platform times changed little, the difference was not statistically significant (P0.05). Factors influencing the changing times of crossing the platform, at different time points the difference was statistically significant (P0.05) between groups and different time; the times of crossing the platform and their interaction (P0.05), time factor with the grouping of different.4, ELISA test in EA group compared with model group, the concentration of VEGF increased significantly after the treatment, there was significant difference between the two groups (P0.01); the effect of the acupoint group and compared to the model group, VEGF increased the applicator after treatment, but no significant difference (P0.05); the effect of electro acupuncture group and the acupoint group compared to the concentration of VEGF increased significantly after EA treatment, the effect of the two groups had significant difference (P0.01). Conclusion: 1, electroacupuncture and moxibustion treatment can reduce blood pressure, longer treatment, blood pressure effect is more obvious, and the antihypertensive efficacy quite.2 two therapy, in behavioral test, after a certain period of treatment, the ability of learning and memory of the rats in the EA group compared with model group, improve a lot. The acupoint group rats improved slightly in the learning and memory function, but the effect is not particularly evident in.3, ELISA test, through a certain period of treatment, the electroacupuncture group VEGF concentration the more obvious, the acupoint group, the concentration of VEGF also has a certain promotion, that both the two kinds of therapy in rats angiogenesis and neuroprotection have certain effect.

【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R245.97

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