针刺治疗脑缺血再灌注损伤机制研究

发布时间：2018-01-03 20:46

本文关键词：针刺治疗脑缺血再灌注损伤机制研究　出处：《中医学报》2017年01期 　论文类型：期刊论文

【摘要】：针刺通过干预丝裂原活化蛋白激酶信号转导通路(mitogen-activated protein kinase,MAPK)信号通路表达治疗脑缺血再灌注损伤机制的相关研究已逐渐开展并初见成效,但仍然存在一些亟待解决的问题,主要表现在:1各研究的造模方式不一,术后评价标准有待进一步考量;2治疗组一般为电针或头针,干预方法较为单调,且取穴、电针参数、观察时间参差不齐,没有统一的标准,具有一定的随意性;3各个研究中观察指标定性定量的检测方法不尽相同,横向可比性不大,而且采用的检测方法也比较传统,今后的实验可以适当创新性采纳先进的技术及设备;4现阶段实验研究对象较局限,绝大部分为各类大鼠,是否能与人体临床实际相一致也有待于将来大量的研究来印证;5现阶段的研究多是针对某单一的MAPK信号通路的家族成员进行观察,而众所周知MAPK家族相当庞大,并且仍然不断有新成员加入,加之生物体内的各种生化反应更是复杂繁琐,将来进一步的实验研究在设计时或许可以考虑同时观察两条、三条甚至更多,以便了解其之间的相互关系,也许针刺正是通过调整各通路之间的动态平衡而发挥对脑缺血再灌注损伤的治疗作用;6当前对于针刺通过干预MAPK信号通路表达治疗脑缺血再灌注损伤机制的相关研究仍然较多地停留在简单的验证及合理的推测上,尤其是对于研究相对较多的细胞外信号调节蛋白激酶信号通路(extracellular signal-regulated kinase,ERK)信号通路的作用至今仍然存在很大争议,这无疑给后续的研究埋下了路障。所以,在该研究领域一些根源性问题上达到共识的要求迫在眉睫,而怎样设计实验建立权威以及更深层次地阐明该机制是对我们提出的一项严峻而意义重大的挑战。
[Abstract]:Acupuncture interferes with mitogen-activated protein kinase via mitogen-activated protein kinase signal transduction pathway. MAPK signal pathway expression in the treatment of cerebral ischemia-reperfusion injury mechanism has been gradually carried out and preliminary results, but there are still some problems to be solved. The main manifestation is the different ways of modeling in each study of 1: 1, and the evaluation criteria need to be further considered. 2 the treatment group is generally electroacupuncture or scalp acupuncture, the intervention method is more monotonous, and points, electroacupuncture parameters, observation time is uneven, there is no uniform standard, with a certain degree of randomness; (3) the qualitative and quantitative detection methods of observation indexes are different, the horizontal comparability is not enough, and the detection methods used are more traditional, so the advanced technology and equipment can be adopted in the future experiments. (4) at present, the objects of experimental study are limited, most of them are all kinds of rats, whether or not they are consistent with the clinical practice of human body needs to be confirmed by a large number of studies in the future. At this stage, most of the studies focus on the family members of a single MAPK signaling pathway, and it is well known that the MAPK family is quite large, and there are still new members to join. In addition, a variety of biological biochemical reactions in the body is more complex and cumbersome, in the future further experimental research in the design may be considered to observe two, three or more, in order to understand the relationship between them. Perhaps acupuncture plays a role in the treatment of cerebral ischemia-reperfusion injury by adjusting the dynamic balance among the pathways. (6) the current studies on the mechanism of acupuncture treatment of cerebral ischemia-reperfusion injury by interfering with the expression of MAPK signal pathway still remain in simple verification and reasonable speculation. In particular, extracellular signal-regulated kinase have been studied more and more in extracellular signal regulated protein kinase (ECK) signaling pathway. The role of ERK signaling pathway is still controversial, which undoubtedly laid a roadblock for further research. Therefore, it is urgent to reach consensus on some root problems in this field. How to design experiments to establish authority and further clarify the mechanism is a serious and significant challenge to us.
【作者单位】：南方医科大学;
【基金】：国家自然科学基金重点项目(81173355)
【分类号】：R246.6
【正文快照】： 脑血管疾病以其发病率高、致残率高、死亡率高、复发率高、并发症多,即“四高一多”的特点已成为我国人口死亡病因最高的头号杀手。流行病学研究显示,我国每年约有200万新发脑卒中的病例[1],其中约70%为缺血性卒中,即脑梗死。脑梗死后50%以上的梗死血管可自发再通,当血流再通

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