补中益气汤“益气升阳”配伍治疗脾虚泄泻大鼠的作用机制研究

发布时间：2018-01-05 07:09

本文关键词：补中益气汤“益气升阳”配伍治疗脾虚泄泻大鼠的作用机制研究　出处：《广州中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的：补中益气汤出自李东垣《脾胃论》,为益气升阳法代表方,是临床上治疗脾虚泄泻证的常用处方。本实验基于病理组织及营养物质吸收的角度,复制脾虚泄泻证动物模型,探讨脾虚泄泻的发生及其发生葡萄糖吸收障碍可能的作用机制；并通过补中益气汤“益气升阳”各配比组方进行干预,明确补中益气汤中益气升阳配比“黄芪-升麻、柴胡”的最佳用量。通过实验研究挖掘其科学内涵,为临床医家运用该方起指导作用。方法：1.补中益气汤益气升阳配比组方对脾虚泄泻大鼠的影响通过多因素法“五味偏食+游泳力竭+控制饮食”建立脾虚泄泻大鼠模型,造模完成后,随机分成补中益气汤组、补中益气汤倍“升麻-柴胡”组及补中益气汤去“升麻-柴胡”组,分别予以相对应的药物进行干预,考察大鼠体重、宏观积分、胸腺指数及脾脏指数。2.补中益气汤益气升阳配比组方对脾虚泄泻大鼠小肠粘膜修复的影响通过多因素法“五味偏食+游泳力竭+控制饮食”建立脾虚泄泻大鼠模型,造模完成后,随机分成补中益气汤组、补中益气汤倍“升麻-柴胡”组及补中益气汤去“升麻-柴胡”组,分别予以相对应的药物进行干预,通过光镜下观察小肠绒毛结构,并运用医学影像分析系统测量小肠绒毛高度及宽度,以及透射电镜观察小肠微绒毛结构。3.补中益气汤益气升阳配比组方对脾虚泄泻大鼠葡萄糖转运体GLUT2相关基因表达的影响通过多因素法“五味偏食+游泳力竭+控制饮食”建立脾虚泄泻大鼠模型,造模完成后,随机分成补中益气汤组、补中益气汤倍“升麻-柴胡”组及补中益气汤去“升麻-柴胡”组,分别予以相对应的药物进行干预后提取小肠粘膜总RNA,运用RT-PCR法检测葡萄糖转运吸收相关基因GLUT2、Na+-K+-ATPase、T1R2+T1R3、Cavl.3、PKC-β-ⅡI亚型基因的表达。成果：1.体重、宏观积分及胸腺、胰脏指数：模型组大鼠体重明显下降,宏观积分明显上升(P0.05)。胸腺指数及脾脏指数明显下降(P0.05)；补中益气汤组体重、胸腺指数、脾脏指数均明显上升,宏观积分下降(P0.05)。而其他两组一些指标上改变不明显。(P0.05)2.病理学检测：模型组大鼠小肠绒毛形态破坏,炎症细胞浸润,上皮细胞缺失；细胞器破坏,小肠绒毛的高度及宽度均明显降低(P0.01)。经补中益气汤干预后补中益气汤组小肠粘膜修复明显,绒毛的高度及宽度均可见升高(P0.01)。而倍“升麻-柴胡”组及去“升麻-柴胡”组病理学上改变不明显(P0.05)。3.葡萄糖转运吸收相关基因：模型组肠道粘膜GLUT2、Na+-K+-ATP酶、PKC-β-Ⅱ亚型表达量明显降低(P0.05)。TIR2、T1R3及Cavl.3表达量亦明显降低(P0.05)。经补中益气汤不同配比组方干预后,各组脾虚泄泻大鼠模型基因表达均有所升高。其中,补中益气汤原方组,各基因的表达量均明显升高(P0.01)。而倍“升麻-柴胡”组及去“升麻-柴胡”组升高不明显(P0.05)。结论：脾虚泄泻大鼠一般状态变化、体重、胸腺指数、脾脏指数下降；小肠绒毛损伤；肠道粘膜葡萄糖转运吸收相关基因表达下降,从而证实了脾虚泄泻的发生与葡萄糖吸收障碍相关,且与肠道粘膜上PKC-β-Ⅱ-GLUT2-TIR2、T1R3/Cav1.3通道密切相关。而补中益气汤通过改善小肠粘膜,提升该通道基因表达,从而达到治疗脾虚泄泻；其中,尤以补中益气汤原方配伍为最佳,进一步证实了补中益气汤益气升阻的配伍应当为益气药与升阳药共用,并且大剂量的益气药与小剂量的升阳药的配伍更能体现其益气升阳功效。
[Abstract]:Objective: Buzhong Yiqi Decoction on spleen and stomach from Li Dongyuan < >, as the representative prescription Yiqi Shengyang method, is commonly prescribed in clinical treatment of diarrhea of spleen deficiency. The pathological tissue and nutrient absorption based on the angle of diarrhea of spleen deficiency animal model replication, to explore possible mechanism and absorption of glucose disorder of spleen deficiency diarrhea; and through BUZHONGYIQITANG "Yiqi Shengyang" the proportion of prescription intervention, clear BUZHONGYIQITANG in the proportion of "Huangqi Yiqi Shengyang - Cimicifuga, bupleurum optimal dosage". Through the experimental study to explore its scientific connotation, guiding role for clinical doctors use the party. Methods: 1. effects of Buzhong Yiqi Decoction prescription of Yiqi Shengyang ratio spleen deficiency diarrhea rats with spleen deficiency diarrhea rat model was established by the method of multi factors "five kinds of diet + swimming + diet", after modeling, were randomly divided into fill in Yiqi Decoction, Buzhongyiqi Decoction times "Cimicifuga - Bupleurum group and Buzhongyiqi Decoction to" Cimicifuga Bupleurum chinense "group, respectively, to the corresponding drug intervention study, rats, macro integral effect, the index of thymus and spleen.2. Buzhongyiqi Decoction Yiqi Shengyang combination ratio on the repair of intestinal mucosa in diarrhea of spleen deficiency rat spleen deficiency diarrhea rat model established by the method of multi factors" five kinds of diet + swimming + diet ", after modeling, were randomly divided into BUZHONGYIQITANG group, BUZHONGYIQITANG times" Cimicifuga - Bupleurum group and Buzhongyiqi Decoction to "Cimicifuga Bupleurum chinense" group, respectively received drug intervention correspondingly, by observation under light microscope, the structure of the intestinal villi, and the use of medical image analysis system to measure the villus height and width, and the TEM microvilli structure.3. BUZHONGYIQITANG Yiqi Shengyang ratio group Influence on related gene expression in rats with spleen deficiency diarrhea glucose transporter GLUT2 of spleen deficiency rat model was established by the method of multi factors "five kinds of diet + swimming + diet", after modeling, were randomly divided into BUZHONGYIQITANG group, BUZHONGYIQITANG times "Cimicifuga - Bupleurum group and Buzhongyiqi Decoction to" Cimicifuga - Bupleurum "group, respectively, to the corresponding drug intervention after extracting total RNA of intestinal mucosa, detected by using the RT-PCR method to absorb glucose transport related genes GLUT2, Na+-K+-ATPase, T1R2+T1R3, Cavl.3, expression of PKC- beta II subtype I gene. Results: 1. body weight, thymus index and macro integration, pancreas weight the rats in the model group decreased significantly, the macro integral increased significantly (P0.05). The index of thymus and spleen decreased significantly (P0.05); BUZHONGYIQITANG group weight, thymus index, spleen index were significantly increased and the macroscopic score decreased (P0 .05). And the other two groups did not change significantly. Some indexes (P0.05) detected 2. pathology: failure modes of small intestine villi of the rats in the model group, the infiltration of inflammatory cells and epithelial cell loss; organelle damage, the height and width of intestinal villi were significantly decreased (P0.01). The BUZHONGYIQITANG group of small intestinal mucosa repair Zhongyi Qi Decoction after the intervention was repaired, the height and width of the villi were found increased (P0.01). And the times "- group and Cimicifuga Bupleurum" to "Cimicifuga pathology - Bupleurum group did not change significantly (P0.05).3. glucose transporter gene related to absorption of intestinal mucosal GLUT2: model group, Na+-K+-ATP enzyme, PKC- beta II subtype expression was lower (P0.05).TIR2, T1R3 and Cavl.3 expression also decreased significantly (P0.05). The different ratio of BUZHONGYIQITANG intervention group, each group of rats with spleen deficiency diarrhea gene increased. Among them, Buzhongyiqi Decoction group, respectively Gene expression levels were significantly increased (P0.01). And the times "Cimicifuga - Chaihu" group and "Cimicifuga Bupleurum chinense was not elevated group (P0.05). Conclusion: the changes of general condition, spleen deficiency diarrhea rat body weight, thymus index, spleen index decreased; intestinal villus injury; intestinal mucosal absorption of glucose transporter gene the expression decreased, thus confirming the occurrence of diarrhea of spleen deficiency and glucose absorption and intestinal mucosal barrier, PKC- beta II -GLUT2-TIR2, T1R3/Cav1.3 channels are closely related. And Buzhongyiqi Decoction by improving intestinal mucosa, enhance the channel gene expression, so as to achieve the treatment of spleen deficiency diarrhea; among them, especially in Buzhongyiqi decoction for compatibility the best, further confirmed the compatibility of BUZHONGYIQITANG lift drag should be shared as Qi Qi medicine and sun medicine, and sun Qi medicine compatibility of high dose and low dose of which can reflect more benefits Gas sun effect.

【学位授予单位】：广州中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R256.34

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