子宫内膜异位症各期血瘀特点研究

发布时间：2018-01-05 19:33

  本文关键词：子宫内膜异位症各期血瘀特点研究　出处：《福建中医药大学》2016年硕士论文　论文类型：学位论文

  更多相关文章： 子宫内膜异位症/ASRM CA125抗原/血清、腹腔液 血瘀积分/分级

【摘要】：目的旨在了解子宫内膜异位症(Endometriosis, EMT)各期的血瘀积分及分级的差异性,并分析不同血瘀积分及分级的EMT患者血清及腹腔液糖类抗原125(CA125)表达差异,以期进一步了解EMT各期的血瘀特点,为临床上更好地中西医结合防治EMT提供客观理论依据。方法1.临床研究：中医采用证素辨证及证素积分的方法,对就诊我院妇科住院部可疑子宫内膜异位症患者,进行问卷调查,计算其血瘀积分及分级,研究EMT各期血瘀特点。2.实验研究：收集经腹腔镜确诊为子宫内膜异位症患者静脉血3mL,并在手术进腹后即取腹腔液2-5mL,位置为子宫膀胱反折腹膜处和子宫直肠陷凹处,血及腹腔液标本均3000r/min离心l0min后取上清液,置-20℃冰箱内保存待测,分析不同血瘀积分及分级的EMT患者血清、腹腔液CA125表达差异。结果1.子宫内膜异位症患者血瘀积分差异性比较：Ⅰ期与Ⅲ期、Ⅳ期,Ⅱ期与Ⅲ期、Ⅳ期的子宫内膜异位症患者血瘀积分对比具有差异性(P0.05)；Ⅰ期与Ⅱ期、Ⅲ期与Ⅳ期的子宫内膜异位症患者血瘀积分对比无差异性(P0.05)。2.子宫内膜异位症患者血瘀分级比较：Ⅰ期与Ⅲ期、Ⅳ期,Ⅱ期与Ⅲ期、Ⅳ期的子宫内膜异位症患者血瘀分级对比具有差异性(P0.05)；Ⅰ期与Ⅱ期、Ⅲ期与Ⅳ期的子宫内膜异位症患者血瘀分级对比无差异性(P0.05)。3.子宫内膜异位症患者血清CA125、腹腔液CA125与血瘀积分进行相关性分析,结果显示两者具有正相关关系,相关系数分别为0.378、0.434(P0.05)。结论1.根据国际ASRM评分标准,将"f15划分为轻型子宫内膜异位症,15划分为中重型子宫内膜异位症,则轻型子宫内膜异位症患者(Ⅰ期与Ⅱ期)或者中重型子宫内膜异位症患者(Ⅲ期与Ⅳ期)血瘀病变的程度没有差异性；而中重型子宫内膜异位症患者的血瘀病变程度则高于轻型子宫内膜异位症患者。2.子宫内膜异位症患者血清CA125、腹腔液CA125与血瘀积分具有相关关系。
[Abstract]:Objective to investigate the difference of blood stasis score and grade of Endometriosissis (EMT) in different stages of endometriosis. The differences of serum and peritoneal fluid carbohydrate antigen 125 CA125 in patients with EMT with different blood stasis scores and grades were analyzed in order to further understand the characteristics of blood stasis in different stages of EMT. In order to provide an objective theoretical basis for the better integration of traditional Chinese and western medicine in the prevention and treatment of EMT methods: 1. Clinical study: syndrome differentiation and syndromes integral method is used in TCM. 2. The patients with suspected endometriosis in gynecological hospital were investigated by questionnaire and their blood stasis scores and grades were calculated. To study the characteristics of blood stasis in different stages of EMT. 2. Experimental study: collect 3 mL venous blood from patients with endometriosis diagnosed by laparoscope, and then take 2 to 5 mL peritoneal fluid after operation. The location was at the retrograde peritoneum of the uterus and bladder and the rectum of the uterus and rectum. The blood and peritoneal fluid samples were centrifuged for 10 minutes, then the supernatant was collected and stored in the refrigerator at -20 鈩，

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