电针足三里、合谷穴对IBS模型大鼠肠道痛敏及动力异常治疗效应的比较研究

发布时间：2018-01-10 09:10

本文关键词：电针足三里、合谷穴对IBS模型大鼠肠道痛敏及动力异常治疗效应的比较研究　出处：《北京中医药大学学报》2017年06期 　论文类型：期刊论文

【摘要】：目的探讨不同经脉及不同神经节段支配的穴位对同一疾病不同症状的疗效差异及相关机制。方法新生Wistar大鼠随机分为空白组、模型组、足三里组和合谷组,每组10~12只。除空白组外,均采用母子分离加醋酸灌肠结合结直肠扩张(CRD)联合制备肠易激综合征(IBS)模型。造模成功者,2月龄时,足三里组、合谷组给予相应穴位电针刺激,20 min/次,隔日1次,共5次。观察各组大鼠电针前后的粪便性状,采用Bristol分型标准评分;腹部回撤反射(AWR)评价内脏痛觉敏感性;免疫组化法检测结肠辣椒素受体(TRPV1)、5-HT2AR的阳性表达。结果与空白组比较,针刺前,模型组、足三里组及合谷组大鼠的Bristol评分均显著升高(P0.01);针刺后,模型组(P0.01)、合谷组(P0.05)大鼠的评分升高;模型组、足三里组腹抬压力阈值明显降低(P0.01),收缩波明显增加(P0.01),结肠TRPV1阳性表达均升高(P0.01),合谷组结肠TRPV1表达增高(P0.05)。模型组、合谷组结肠5-HT2AR阳性表达升高(P0.01)。与模型组比较,足三里组及合谷组,针刺后Bristol大便评分降低(P0.01);腹抬压力阈值升高(P0.01)、收缩波个数减少(P0.01);结肠TRPV1、5-HT2AR阳性表达均显著性降低(P0.01)。与足三里组相比,合谷组针后Bristol评分升高(P0.05),结肠TRPV1表达下降(P0.01),5-HT2AR表达升高(P0.01)。结论电针足三里穴和合谷穴均能降低IBS大鼠肠道痛敏感性,改善胃肠动力障碍。两穴比较,合谷穴对IBS内脏痛的治疗效应略优于足三里穴,而足三里穴对胃肠运动的调节效应更具优势。说明不同经脉、不同神经节段支配的穴位可以治疗同一病症,但存在效应差异,证实了经穴效应特异性存在且具有相对性。
[Abstract]:Objective to explore the therapeutic effects and related mechanisms of different acupoints innervated by different meridians and different nerve segments on the same disease and different symptoms. Methods newborn Wistar rats were randomly divided into blank group and model group. In Zusanli group and Hegu group, 12 rats were in each group, except the blank group. The IBS model of irritable bowel syndrome (IBS) was established by the combination of mother and child separation with acetic acid enema combined with colorectal dilatation (CRD). The successful rats were treated with Zusanli group at the age of 2 months. In Hegu group, the corresponding acupoints were stimulated with electroacupuncture for 20 minutes every time, once every other day for 5 times. The fecal characters of rats before and after electroacupuncture were observed, and the scores of Bristol classification criteria were used. Abdominal withdrawal reflex (AWR) was used to evaluate visceral pain sensitivity. Immunohistochemical method was used to detect the positive expression of 5-HT _ 2AR in colonic capsaicin receptor TRPV1.Results compared with the blank group, the model group before acupuncture. The Bristol scores of Zusanli group and Hegu group were significantly higher than those of Hegu group. After acupuncture, the score of model group was higher than that of Hegu group (P 0.05). In the model group, the ventral pressure threshold of Zusanli group decreased significantly (P 0.01), the contraction wave increased significantly (P 0.01), and the positive expression of TRPV1 in colon increased significantly (P 0.01). Compared with model group, Zusanli group and Hegu group, Zusanli group and Hegu group had higher expression of 5-HT2AR. The score of Bristol stool decreased after acupuncture (P 0.01). The threshold of abdominal lifting pressure increased P0.01a, and the number of contraction waves decreased P0.01a; Compared with Zusanli group, the Bristol score of Hegu group was higher than that of Zusanli group (P 0.05). The expression of TRPV1 in colon decreased P0.01A 5-HT 2AR expression increased. Conclusion electroacupuncture at Zusanli point and Hegu point can reduce the sensitivity of intestinal pain in IBS rats. The therapeutic effect of Hegu point on visceral pain of IBS was slightly better than that of Zusanli point, while Zusanli point had more advantages in regulating gastrointestinal motility. The acupoints innervated by different nerve segments can treat the same disease, but there are differences in the effects, which proves that the effects of the meridians are specific and relative.
【作者单位】：北京中医药大学;
【基金】：国家自然科学基金项目(No.81473776)~~
【分类号】：R245;R-332
【正文快照】： 吴艳英,女,在读硕士生(北京中医药大学北京100029)tal distension(CRD)test to rats in all groups except the normal control group.These rats were all isolatedfrom their mothers.2-month old rats in the ST36 group and LI14 group received electro-acupuncture five

【相似文献】