培元固本治法的理论探讨及其对肾虚型AD大鼠新生神经元的作用研究

发布时间：2018-01-10 12:08

  本文关键词：培元固本治法的理论探讨及其对肾虚型AD大鼠新生神经元的作用研究　出处：《湖北中医药大学》2017年硕士论文　论文类型：学位论文

  更多相关文章： 元气 肾虚痴呆 神经发生

【摘要】：目的:元气是维持生命活动的基础物质,是人体生命活动的原动力。元气禀受于父母先天之精,藏之于肾和命门。现代流行病学研究也表明,AD患者中以肾虚证最为常见。中医认为“肾虚髓空”是AD的根本,并把“从肾论治”作为主要治疗法则。本课题从培补元气出发,以肾虚型老年痴呆为切入点,探讨元气与肾虚型老年痴呆的关系以及培元固本治法对肾虚型老年痴呆的作用。以肾虚型老年痴呆大鼠为动物模型,观察培元固本复方长生固本方、固本健脑液及培元固本胶囊对肾虚型痴呆模型大鼠海马内Aβ_(1-40)、Aβ_(1-42)的聚集情况以及齿状回新生神经元增值和成熟的研究,旨在为临床治疗肾虚型老年痴呆提供理论依据和实验数据。方法:1.理论探讨:收集整理元气与肾虚型老年痴呆的相关文献以及痴呆患者海马内新生神经元增殖及成熟的相关文献,探讨从培补元气论治肾虚型AD的理论依据。2.实验研究:以肾虚型AD大鼠为动物模型,以海马内新生神经元增值与成熟的情况为探讨对象,来研究培元固本中药复方长生固本方、固本健脑液及培元固本胶囊对肾虚型痴呆模型大鼠的作用。用体重、皮质酮、睾酮和水迷宫的数据评价肾虚型老年痴呆模型;用Morris水迷宫定位航行实验和空间探索实验检测模型大鼠的学习记忆能力;ELISA法检测固本三方对大鼠海马组织Aβ_(1-40)和Aβ_(1-42)蛋白含量的影响;Brdu免疫组化分析固本三方对肾虚型AD大鼠模型新生神经元增值情况。结果:1.体重及皮质酮、睾酮含量变化:与空白组比较,模型组大鼠体重随着氢化可的松注射没有增重甚至减轻;与空白组比较,模型组血清中皮质酮含量明显低于空白组(p0.05);与空白组比较,模型组血清中睾酮含量显著低于空白组(p0.01)。结合体重及一般情况,肾虚模型造模成功。2.Morris水迷宫测试结果显示:与模型组比较,各组大鼠上平台潜伏期均缩短(p0.01,p0.05),游泳总路程均减少(p0.01,p0.05),与多奈哌齐组比较,长生固本方组、固本健脑液组上平台潜伏期和游泳总路程都没有明显差异(p0.05),培元固本胶囊组上平台潜伏期无明显变化(p0.05),游泳总路程增加(p0.05);相比模型组,其余各组大鼠的穿越平台总数量及目标象限时间都显著增多(p0.01,p0.05),第一次穿越平台时间均明显缩短(p0.01,p0.05)。与多奈哌齐组比较,长生固本方组大鼠在固定象限停留时间及第一次抵达平台时间均没有明显区别(p0.05),经过平台区的次数相对减少(p0.05);固本健脑液组大鼠穿越平台次数及目标区域总时间没有明显区别(p0.05),第一次抵达平台时间相对较长(p0.01);培元固本胶囊组穿越平台时间无差异(p0.05),目标区域总时间缩短(p0.05),第一次抵达平台时间相对较长(p0.05)。3.大鼠海马组织Aβ_(1-40)和Aβ_(1-42)蛋白的含量:与比模型组,空白组、多奈哌齐组、长生固本方组大鼠海马组织内Aβ_(1-40)含量明显降低(p0.01),固本健脑液组和培元固本胶囊组大鼠海马组织内Aβ_(1-40)含量降低(p0.05);与多奈哌齐组比较,长生固本方组Aβ_(1-40)含量无明显异常(p0.01),固本健脑液组和培元固本胶囊组Aβ_(1-40)含量升高(p0.05);相比模型组,空白组、多奈哌齐组、长生固本方组、固本健脑液组大鼠海马组织内Aβ_(1-42)含量明显降低(p0.01),培元固本胶囊组大鼠海马组织内Aβ_(1-42)含量降低(p0.05);与多奈哌齐组比较,长生固本方组、固本健脑液组Aβ_(1-42)含量无明显异常(p0.01),培元固本胶囊组Aβ_(1-42)含量升高(p0.05)。4.新生神经元成熟细胞数结果比较:与模型组比较,空白组、多奈哌齐组、长生固本方组和固本健脑液组其新生神经元成熟细胞数增多(p0.05),而培元固本胶囊组新生神经元成熟细胞数未见明显区别(p0.05)。与多奈哌齐组比较,长生固本方组、固本健脑液组及培元固本胶囊组无差异(p0.05)。结论:1.造模结束后,模型组大鼠体重增加不明显,甚至出现体重下降,活跃程度低,不爱活动,便稀软,颤栗,被毛炸开并脱落明显等现象。随着药物的延续,逐渐出现活动度差、便溏、怕冷、竖毛、毛发光泽度差等症状;结合记忆学习能力水平大幅度下降,判断本实验造模成功。2.长生固本方、固本健脑液及培元固本胶囊均能在一定程度上改善模型大鼠的记忆学习功能,并且无明显差异。3.长生固本方、固本健脑液及培元固本胶囊均能在一定程度上降低海马组织内的Aβ_(1-40)和Aβ_(1-42)蛋白的含量,长生固本方、固本健脑液较培元固本胶囊作用佳。4.长生固本方、固本健脑液及培元固本胶囊均能促进新生神经元的增值和成熟,并且无明显差异,能有效改善痴呆症状。
[Abstract]:Objective: Qi is the basic substance to maintain life activities, is the primary power of life vitality from parents. The congenital essence hidden in kidney and the life gate. Epidemiological studies also showed that AD patients with kidney deficiency syndrome is the most common. TCM believes that "kidney marrow empty" is the root of the AD, and "from renal differentiation" as the main treatment rules. This topic from the reinforcing primordial qi of kidney deficiency in the senile dementia as the breakthrough point, to explore the relationship between Qi and kidney deficiency type of senile dementia and Peiyuan treatment of kidney deficiency type senile dementia. In kidney deficiency type senile dementia rats as animal model, to observe the school the compound longevity Guben decoction, the physic liquid and Peiyuan Capsule on kidney deficiency in hippocampus of dementia model rats in A beta _ (1-40), A (1-42) _ beta aggregation and dentate gyrus neurons and mature research value, in order for clinical treatment Senile kidney deficiency and provide a theoretical basis and experimental data. Methods: 1. theoretical study: collecting related literature spirit and kidney deficiency in the elderly dementia and dementia in hippocampus neurons proliferation and maturation of related literature, study of the theoretical basis of.2. from reinforcing the strength on the treatment kidney deficiency type AD: kidney deficiency type AD rat as the animal model in the hippocampus of newborn neurons increase and the mature as the object of study, to study the traditional Chinese medicine compound Peiyuan Guben longevity, the physic liquid and Peiyuan Capsule on kidney deficiency dementia model in rats. Body weight, cortisol, testosterone and water maze data model evaluation of kidney deficiency type of senile dementia; learning and memory by Morris water maze navigation experiment and space exploration test model rats; ELISA assay of the three party of rat hippocampus A beta _ (1-40) and A (1- _ beta 42) the effect of protein content; Brdu immunohistochemical analysis of the three party of kidney deficiency rat model of AD neurons value. Results: 1. body weight and cortisol, testosterone content changes: compared with the control group, body weight of model rats with hydrocortisone injection did not even reduce weight gain compared with the control group;, significantly lower than the control group serum corticosterone levels in the model group (P0.05); compared with the control group, the serum testosterone level in the model group was significantly lower than that of the control group (P0.01). Combined with the weight and general condition, kidney deficiency models.2.Morris water maze test results showed that: Compared with the model group, the rats on the platform latency (P0.01, P0.05) was shortened, the swimming distance were reduced (P0.01, P0.05), compared with donepezil group, the longevity Group, have no difference in the physic liquid group on the platform latency and swimming distance (P0.05), Peiyuan Capsule group on the platform with no obvious change in latency (P0.05), total swimming distance increased (P0.05); compared with the model group, the rats of the total number of crossing platform and target quadrant time increased significantly (P0.01, P0.05), the first cross platform time were significantly reduced (P0.01, P0.05). Compared with donepezil group the solid, longevity rats in the fixed quadrant time and the first time to reach the platform had no significant difference (P0.05), after the number of platform area is relatively reduced (P0.05); the physic liquid group rats through the total time and the number of target platform has no obvious difference (P0.05), the first time to reach the platform a relatively long time (P0.01); Peiyuan capsule group no difference across the platform (P0.05), the target area total time (P0.05), the first time arrived at the platform for a relatively long time (P0.05) of.3. in the hippocampus of rats A beta _ (1-40) and A (1-42) protein beta _ 鐨勫惈閲，

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