RP长链RNA表达特征及窍明穴排刺法联合通窍明目Ⅲ号治疗RP的干预作用

发布时间：2018-01-10 15:32

本文关键词：RP长链RNA表达特征及窍明穴排刺法联合通窍明目Ⅲ号治疗RP的干预作用　出处：《黑龙江中医药大学》2016年博士论文　论文类型：学位论文

【摘要】：目的：观察窍明穴排刺法联合中药方剂通窍明目Ⅲ号对原发性视网膜色素变性(RP)的疗效,研究针药并用对RP视网膜结构的干预作用和窍明穴排刺法的优效性。通过观察视力、视野的变化及OCT对视网膜神经纤维层厚度的影响,研究针药并用对RP视功能的影响及其可能的作用机制。方法：对选择诊断纳入标准,共60例119眼的RP患者随机分为两组,治疗组：窍明穴排刺法联合通窍明目Ⅲ号；对照组：除窍明穴外的其他穴位针法联合通窍明目Ⅲ号,对视力,视野及对视网膜神经纤维层厚度的影响,进行治疗前后的组内及组间比较。通过观察治疗前后视力、视野和OCT的影响,评价窍明穴排刺法联合通窍明目Ⅲ号对RP的治疗效果。结果：1.治疗前后实验组与对照组视力比较：治疗组有效率为61.67%,对照组有效率为44.07%,差异有统计学意义(P0.05)；2.治疗前后实验组与对照组视野平均缺损比较：治疗组治疗前视野平均缺损值为17.79±4.07,治疗后为11.03±4.824；对照组治疗前16.39±5.34,治疗后为12.05±4.889。经统计学检验,证明两组差异均有统计学意义(P0.05)；3.治疗前后实验组与对照组的OCT对视盘周边平均厚度比较：治疗组总有效率为58.33%,对照组总有效率为54.24%。两组数据有差异,经检验无统计学意义(P0.05)。结论：窍明穴排刺法联合通窍明目Ⅲ号治疗RP可以提高视力,扩大视野；表明经短期治疗可改善视功能,形态学改变不明显。目的：通过探索RP及其他眼病患者LncRNA特征性表达谱,探求在RP LncRNA的外周血中潜在调控的基因通路,对RP疾病的早期临床诊断提供新思路。方法：1.样本取自黑龙江中医药大学附属第一医院眼科病房3例RP患者外周血设为实验组,3例正常人的外周血样本设为对照组。采用Arraystar公司的人类LncRNA芯片V3.0。运用该芯片检测了30586个LncRNA和26109个LncRNA蛋白编码基因的转录,并进行本实验研究受试样品的人类LncRNA和蛋白编码转录物的高通量差异表达分析。2.RNA标记和阵列杂交应用酚氯仿法提取总RNA。然后,将每个样品逆转录的cRNA通过RNeasy Mini试剂盒进行纯化和浓度测定。应用mRNA芯片对cRNA进行杂交标记,进而洗涤,固定和使用安捷伦DNA微阵列扫描仪(部件号G2505C)扫描。3.数据分析使用安捷伦软件11.0.1.1分析获得的阵列图像并用GeneSpring GXV12.1软件包进行分位数标准化数据处理。将原始数据LncRNAs 和 mRNA的标准化之后用于进一步的数据分析。从而筛选出具有表达差异的数据。通过Pathway分析和GO分析,LncRNAs/mRNA确定这些生物学通路中的调控作用。结果：本实验通过对样品的人类LncRNA和蛋白编码转录物进行聚类、富集分析等高通量差异表达分析,发现LncRNA主要涉及RP疾病的表达,得到LncRNA、mRNA参与了基因分子及基因的部分相互作用,并发现基因的表达模式和调节表达的信号通路。结论：LncRNA在RP外周血中,存在特征性表达谱具有与发病相关的临床意义。
[Abstract]:Objective: To observe the point orifices row needling therapy combined with traditional Chinese medicine tqmm No. 3 of retinitis pigmentosa (RP) curative effect and superiority of acupuncture and medicine on RP retinal structure and intervention effect of acupuncture point discharge orifices Ming. By observing the visual acuity, changes and effects of OCT on the horizon the retinal nerve fiber layer thickness, the research of drugs and acupuncture effects on visual function of RP and its possible mechanism. Methods: the diagnostic criteria, a total of 60 cases of RP patients were randomly divided into two groups of 119 eyes, treatment group: acupoint needling combined with Qiao Ming Pai tqmm III; control group: in addition to the point outside the other orifices acupoint acupuncture combined with tqmm No. 3, on the vision, vision and effect on retinal nerve fiber layer thickness, compared before and after treatment group and group were observed before and after treatment. The visual acuity, visual field and OCT effect, evaluation point needling row orifices Therapeutic effect of combined with tqmm No. 3 on RP 1. before and after treatment. Results: the experimental group and control group was compared: the treatment group was 61.67%, the control group was 44.07%, the difference was statistically significant (P0.05; 2.) before and after treatment in the experimental group and the control group mean defect of visual field comparison: the treatment group before the treatment field the average defect value was 17.79 + 4.07, 11.03 + 4.824 after treatment; the control group before treatment after treatment for 16.39 + 5.34, 12.05 + 4.889. proved by statistical test, there were significant differences between two groups (P0.05; 3.) before and after treatment in experimental group and control group OCT of optic disc surrounding the average thickness of comparison: treatment group total efficiency is 58.33%, the total efficiency of the control group for the 54.24%. data of the two groups had no significant difference, after inspection (P0.05). Conclusion: acupuncture needling combined with row orifices Ming tqmm No. 3 RP treatment can improve visual acuity, enlarging horizon; Show that the short-term treatment can improve the visual function and morphological changes are not obvious. Objective: To explore the RP features of patients with LncRNA ophthalmopathy and other gene expression profiles in RP LncRNA to explore the potential pathway in peripheral blood of control, to provide new ideas for early diagnosis of RP disease. Methods: 1. samples were taken from the ophthalmology ward of the First Affiliated Hospital of Heilongjiang University Of Chinese Medicine, 3 cases of peripheral blood of RP patients as the experimental group, 3 cases of normal peripheral blood samples as control group. The Arraystar human LncRNA chip V3.0. use of the chip to detect the transcription of 30586 LncRNA and 26109 LncRNA protein encoding gene, and high-throughput protein encoding human LncRNA and transcription the differences in the experimental study of samples for total RNA. extraction and.2.RNA marker analysis and array hybridization application of phenol chloroform method expression, each sample of cRNA RNeasy by reverse transcription M Ini kit for purification and concentration. The application of the mRNA chip of cRNA hybridization, and then washing, fixing and using the Agilent DNA microarray scanner (Part No. G2505C) using Agilent 11.0.1.1 array analysis of images obtained by GeneSpring and GXV12.1 software package for data processing standard digit scanning.3. after LncRNAs and data analysis. MRNA original data standardization for further data analysis. In order to find out the difference with expression data. Through the analysis of Pathway and GO, LncRNAs/mRNA to determine the biological role through in the road. Results: the experiment was carried out based on clustering samples of human LncRNA and protein encoding transcripts, expression analysis of enrichment analysis of high flux the difference, LncRNA expression was found mainly related to RP disease LncRNA, mRNA gene is involved in molecular and gene of each other Conclusion: LncRNA has characteristic clinical expression in peripheral blood of RP, which is associated with pathogenesis.

【学位授予单位】：黑龙江中医药大学
【学位级别】：博士
【学位授予年份】：2016
【分类号】：R276.7

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