回药扎里奴思方对痰热腑实脑缺血模型大鼠血脂、血浆同型半胱氨酸及内皮素-1的影响

发布时间：2018-01-12 22:28

本文关键词：回药扎里奴思方对痰热腑实脑缺血模型大鼠血脂、血浆同型半胱氨酸及内皮素-1的影响　出处：《中国老年学杂志》2017年12期 　论文类型：期刊论文

【摘要】：目的探讨回药扎里奴思方对痰热腑实脑缺血急性期再灌注模型大鼠脂质代谢、血浆同型半胱氨酸(Hcy)及内皮素(ET)-1的影响。方法将SD大鼠随机分为正常组,假手术组,单纯脑梗死组,痰热腑实模型组,尼莫地平组,扎里奴思方组,采用10%的自体粪便灌胃加高脂饮食的方法复制痰热腑实动物模型,采用改良线栓法复制脑缺血再灌注(MCAO)模型。再灌注2 h后按相应分组灌胃给药,观察大鼠神经功能评分及行为学改变,心尖取血测其血清中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、Hcy含量,免疫组化法及酶联免疫标记法测定脑组织中ET-1的含量。结果与模型组比较,各用药组神经功能缺损及痰热腑实症状明显改善,血清中TC、TG、HDL-C、LDL-C、Hcy及ET-1含量显著降低。结论扎里奴思方可降低痰热腑实脑缺血再灌注模型大鼠急性期血脂及Hcy水平,减少ET-1的表达,具有保护脑神经细胞的作用,并对痰热腑实型脑缺血有明显的改善作用。
[Abstract]:Objective to investigate the medicine zlnsf of phlegm heat acute cerebral ischemia reperfusion rat model of lipid metabolism, plasma homocysteine (Hcy) and endothelin (ET) -1. Methods SD rats were randomly divided into normal group, sham operation group, the pure cerebral infarction group, phlegm heat accumulation the model group, nimodipine group, zlnsf group, using the method of autologous feces 10% lavage and high fat diet copy of phlegm heat animal model, using a modified suture method to duplicate cerebral ischemia reperfusion (MCAO) model. 2 h after reperfusion in corresponding group gavage, observation of nerve rats function score and behavioral changes, apex blood serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), the content of Hcy, ET-1 in brain tissue was measured by immunohistochemistry and ELISA marker the results with the model. Group, each group of neural function defect and phlegm heat symptoms improved significantly, serum TC, TG, HDL-C, LDL-C, Hcy and ET-1 content decreased significantly. Conclusion Zardari slave think party can reduce phlegm heat and blood lipid levels in cerebral ischemia Hcy reperfusion in acute model rats, reduce ET-1 the expression can protect brain nerve cells, and has obvious effect on improving the real type of cerebral ischemia syndrome of phlegm heat.

【作者单位】：宁夏医科大学国医药现代化省部共建教育部重点实验室;
【基金】：“十二五”国家科技支撑计划项目基金(No.SQ2013SF12E02181) 国家自然科学基金(No.81260569)
【分类号】：R29
【正文快照】： 痰热腑实证是脑缺血急性期最常见的证型[1,2],中风急性期,痰瘀互结阻滞经络,加之风火热毒灼伤津液导致肠燥津亏,大便秘结,形成痰热腑实证;其发病与血脂、同型半胱氨酸(Hcy)水平及血管内皮素(ET)-1密切相关。ET-1是目前已知作用最强的血管收缩剂,在脑卒中急性期,ET-1是脑血管损

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