利用微透析技术测定火针对类风湿性关节炎大鼠病变组织中5-羟色胺、组胺和前列腺素E2的影响

发布时间：2018-01-13 03:26

本文关键词：利用微透析技术测定火针对类风湿性关节炎大鼠病变组织中5-羟色胺、组胺和前列腺素E2的影响　出处：《暨南大学学报(自然科学与医学版)》2017年04期 　论文类型：期刊论文

【摘要】：目的:观察不同干预方法对风湿性关节炎模型大鼠病变组织中5-羟色胺(5-HT)、组胺(HA)和前列腺素E2(PGE_2)含量影响与镇痛效应,探讨火针缓解类风湿性关节炎慢性疼痛的作用机制.方法:将健康雄性SD大鼠50只,随机分为正常对照组、模型组、针刺组、穴位埋线组和火针组,各10只,以完全弗氏佐剂(CFA)注入大鼠右后足垫皮肤内制作类风湿性关节炎模型,3组均取右侧"昆仑"、"阳陵泉"作为治疗穴位,分别行火针、普通针刺、穴位埋线治疗,利用微透析采样技术(MD)在线提取大鼠局部组织液,结合HPLC-UV检测技术,监测类风湿性关节炎模型大鼠不同时段内局部病变组织5-HT、HA和PGE_2含量的变化.结果:造模后大鼠肌肉组织中5-HT、HA和PGE_2含量较正常组明显增高,热刺痛爪退缩阈值降低(P0.01).治疗后,各组大鼠TWL阈值升高,病变肌肉组织内5-HT、HA、PGE_2含量较治疗前均有不同程度下降,火针组5-HT、HA、PGE_2含量分别在2、4、8 h处于低水平状态,与模型组比较有显著性差异(P0.01);普通针刺组5-HT、HA、PGE_2含量分别在1、2 h处于低水平状态;穴位埋线在治疗前期与模型组无统计学差异(P0.05),该组下降时间点分别于治疗后2、4 h,与模型组有统计学差异(P0.05).结论:火针、普通针刺、穴位埋线均可以降低局部肌肉组织内5-HT、HA和PGE_2含量;火针镇痛效应优于普通针刺组和穴位埋线组,且火针首次即时镇痛维持时间长于普通针刺组和穴位埋线组,穴位埋线则至少需要2 h以上才能发挥镇痛效应.
[Abstract]:Objective: to observe the effect and analgesic effect of different intervention methods on the contents of 5-hydroxytryptamine (5-HT) and prostaglandin E _ 2 (PGE _ 2) in pathological tissues of rats with rheumatoid arthritis. Methods: 50 healthy male SD rats were randomly divided into normal control group, model group, acupuncture group, acupoint embedding group and fire acupuncture group. 10 rats in each group were injected with complete Freund's adjuvant (CFAA) into the skin of right hind foot pad to make rheumatoid arthritis model. The right "Kunlun" and "Yanglingquan" were taken as treatment points respectively. Common acupuncture, catgut embedding therapy at acupoint, microdialysis sampling technique was used to extract the local tissue fluid of rats on line, combined with HPLC-UV detection technique. The changes of 5-HT HA and PGE_2 in local pathological tissues of rats with rheumatoid arthritis were monitored in different periods. The content of HA and PGE_2 was significantly higher than that of the normal group, and the threshold of retraction of hot stinging claw was decreased (P 0.01). After treatment, the threshold of TWL was increased and 5-HT HA was found in the diseased muscle tissue of rats in each group. The content of PGE_2 was decreased in different degree compared with that before treatment, and the content of 5-HT Ha-Ha-PGE2 in fire needle group was at a low level at 2h for 4h. There was significant difference between the model group and the model group (P 0.01). The content of 5-HTHA-PGE2 in normal acupuncture group was in low level at 1h and 2h, respectively. There was no statistical difference between the acupoint embedding group and the model group before treatment. The decrease time point of the group was 2 hours after treatment, and there was a statistical difference between the model group and the model group. Conclusion: fire acupuncture. The content of 5-HT HA and PGE_2 in local muscle tissue was decreased by acupuncture and catgut embedding at acupoint. The analgesic effect of fire acupuncture group was better than that of common acupuncture group and acupoint embedding group, and the duration of fire acupuncture immediate analgesia was longer than that of common acupuncture group and acupoint embedding group.
【作者单位】：深圳市宝安区中医院针灸科;深圳市第六人民医院南山医院康复科;广州中医药大学第一附属医院针灸科;
【基金】：国家自然科学基金项目(81574061) 深圳市卫计委博士创新基金项目(201605025)
【分类号】：R245
【正文快照】： 类风湿性关节炎(rheumatoid arthritis,RA)是一种以侵蚀性关节炎为主要表现的全身性自身免疫病,主要病理变化为关节滑膜的慢性炎症.病变侵犯人体多个关节,一般以手足小关节起病,呈对称性,也可侵犯其他系统变生他病[1],其发病率与年龄呈正相关[2],致残率高,长期不愈可致畸,严重

【相似文献】