以2型糖尿病为对象探讨厚苔形成相关调节蛋白的表达

发布时间：2018-01-15 08:15

本文关键词：以2型糖尿病为对象探讨厚苔形成相关调节蛋白的表达　出处：《北京中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:舌诊是中医望闻问切四诊合参的重要组成部分,是辨证论治的基础,对中医诊断疾病、辨证分型、遣方用药、判定预后具有重要的指导意义。近年来,对于舌诊客观化以及与疾病相关性分析的研究越来越多。本研究以初发2型糖尿病厚苔患者为研究对象,探讨糖尿病厚苔形成相关的调节蛋白分子机制。方法:于中国中医科学院广安门医院招募初发2型糖尿病厚苔以及薄苔患者共72名,以及健康人群厚苔以及薄苔者60名为对照组,留取空腹血清行ELISA检测EGF、E-cad等蛋白含量,留取舌苔标本行PCR检测EGF、E-cad等基因含量,分析组间相关性以及与血糖、血脂、BMI水平的相关性。另招募初发糖尿病患者6例,其中厚苔3例薄苔3例,以及6名健康人厚薄苔对照组,留取舌苔标本行16s rDNA微生物测序,检测糖尿病患者与健康人不同厚薄舌苔样本中微生物菌群丰度以及多样性。结果:1、血清ELISA检测:2型糖尿病厚苔和薄苔患者中,E-cad表达水平具有统计学意义;健康人薄厚苔中,E-cad与EGF表达水平具有统计学意义;2型糖尿病厚苔与健康人厚苔,E-cad与EGF表达水平具有统计学意义;2型糖尿病薄苔与健康人薄苔,E-cad表达水平具有统计学意义;糖尿病厚苔组与健康厚苔组间CH0、TG、HDL、LDL比较差异有统计学意义;糖尿病薄苔与健康薄苔组间CHO、TG、LDL比较差异有统计学意义;糖尿病厚苔与健康组薄苔之间CH0、TG、LDL比较差异有统计学意义;BMI值比较,糖尿病厚、薄苔组间、糖尿病厚苔与健康对照厚苔组间、糖尿病厚苔组与健康薄苔组间比较,差异均有统计学意义。2、PCR检测:E-cad在糖尿病厚苔组与薄苔组之间具有统计学意义,在糖尿病薄苔中E-cad含量含量低,厚苔中含量高;同时发现目标基因包括内参GAPDH于舌苔中含量较低。3、16s rDNA测序共检测出130余个OTUs,初步统计过后,发现Granulicatella在糖尿病薄苔比健康人厚苔和薄苔含量高、健康人薄苔比健康人厚苔含量高;Rothia在健康人厚苔中和糖尿病厚苔患者中明显偏高。结论:E-cad在2型糖尿病、薄苔患者中高表达,说明E-cad可能与糖尿病以及厚苔形成具有相关性;综合ELISA血清检测以及PCR舌苔检测发现,E-cad对于糖尿病以及舌苔厚度具有一定的差异性;血脂、BMI与糖尿病相关,与舌苔厚薄无关;Granulicatella、Rothia在舌苔微生物菌群中具有差异性,具体机制有待进一步研究。
[Abstract]:Objective: tongue diagnosis is an important part of traditional Chinese medicine, which is the basis of syndrome differentiation and treatment. It has an important guiding significance in the diagnosis of diseases, syndrome differentiation, prescription medication and prognosis in recent years. There are more and more researches on the objectification of tongue diagnosis and the analysis of correlation between tongue diagnosis and disease. This study focuses on the patients with thick moss of primary type 2 diabetes mellitus (T2DM). Methods: 72 patients with type 2 diabetes mellitus were recruited in Guanganmen Hospital of Chinese Academy of traditional Chinese Medicine. And 60 healthy people with thick and thin fur were taken as control group. Fasting serum was taken to detect the content of EGF-E-cad and PCR was used to detect EGF in tongue coating samples. The content of E-cad gene, the correlation between the analysis group and the blood sugar, blood lipid and BMI were analyzed. 6 patients with primary diabetes mellitus were recruited, including 3 cases of thick fur and 3 cases of thin fur. The specimens of tongue coating were collected from 6 healthy subjects and the control group were collected. The samples were sequenced by 16s rDNA microorganism. The microbial flora abundance and diversity in different thick and thin tongue coating samples from diabetic patients and healthy people were detected. Results: 1. Serum ELISA was used to detect thick fur and thin fur patients with type 2 diabetes mellitus. The expression level of E-cad was statistically significant. The expression levels of E-cad and EGF in healthy persons were statistically significant. The expression of E-cad and EGF in type 2 diabetes mellitus was significantly higher than that in healthy subjects. The expression of E-cad in type 2 diabetes mellitus was significantly higher than that in healthy subjects. There was a significant difference in HDLL-LDL between the CH0 TGG and the healthy thick fur group in the diabetes mellitus (DM) group and the control group (P < 0.05). There was significant difference in TGG LDL between diabetic thin fur group and healthy thin fur group. There was a significant difference in TGG LDL between the thick fur of diabetes mellitus and the thin fur of the healthy group. There were significant differences in BMI values between the diabetic thick fur group and the thin fur group, between the diabetic thick fur group and the healthy control group, and between the diabetic thick fur group and the healthy thin fur group. PCR analysis showed that the content of E-cad was lower in the diabetic thin fur and higher in the thick fur than that in the thin one. At the same time, it was found that the target gene, including the content of GAPDH in tongue coating, was low. 3C16 s rDNA sequencing detected more than 130 OTUs. After preliminary statistics, more than 130 OTUs were detected. It was found that the content of Granulicatella in diabetic thin fur was higher than that in healthy persons, and that in healthy persons was higher than that in healthy persons. Rothia was significantly higher in healthy persons with thick fur and diabetic patients with thick fur. Conclusion the expression of E-cad is high in type 2 diabetes mellitus and thin fur patients. These results suggest that E-cad may be associated with diabetes mellitus and the formation of thick moss. Combined with ELISA serum test and PCR tongue coating test, it was found that E-cad had some difference in diabetes mellitus and tongue coating thickness. BMI of blood lipids was correlated with diabetes mellitus, but not with thickness of tongue coating. Granulicatellaa Rothia is different in the microflora of tongue coating, and the specific mechanism needs further study.
【学位授予单位】：北京中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R259

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