脊柱推拿干预脑瘫幼鼠生长发育及运动功能的时间量效实验研究

发布时间：2018-01-16 10:32

本文关键词：脊柱推拿干预脑瘫幼鼠生长发育及运动功能的时间量效实验研究　出处：《时珍国医国药》2017年09期 　论文类型：期刊论文

【摘要】：目的观察不同时长的脊柱推拿对脑瘫幼鼠生长发育及运动功能的影响,探究推拿治疗时间与疗效的关系。方法将新生40只SD幼鼠随机分为空白对照组(n=8)和实验组(n=32);实验组又随机分为4个亚组:模型对照组(n=8)和模型+推拿1组(n=8),模型+推拿2组(n=8),模型+推拿3组(n=8)。模型制备:幼鼠出生后第3天(3-day-postnatal,P3),采用单侧颈总动脉结扎联合缺氧缺血的方法制备动物模型,造模后1d(P4)开始进行脊柱推拿;各组在相同的时间点检测幼鼠的体重和睁眼时间,并进行倾斜板实验。结果空白对照组与模型对照组相比,体重、睁眼时间以及倾斜板实验结果皆具有明显统计学差异,表明造模成功。与模型对照组相比,模型+推拿1、模型+推拿2、模型+推拿3组幼鼠体重明显增加(P0.05),推拿组组间两两对比并无统计学显著差异(P0.05);睁眼时间:模型对照组模型+推拿1组模型+推拿2组、模型+推拿3组,除模型+推拿2组与模型+推拿3组比较无显著统计学差异外(P=0.285),各组间两两比较均有显著的统计学差异(P0.05)。倾斜板角度:模型+推拿2组模型+推拿3组模型+推拿1组模型对照组,各组间两两比较均有显著的统计学差异(P0.05)。结论脊柱推拿对缺氧缺血型脑瘫幼鼠的生长发育及运动功能恢复具有明显的促进作用;推拿的时长并非与效果成正比,选择适宜的时长进行推拿,效果更佳。
[Abstract]:Objective to observe the effect of spinal massage with different length on growth and motor function of young rats with cerebral palsy. Methods Forty newborn Sprague-Dawley rats were randomly divided into control group (n = 8) and experimental group (n = 32). The experimental group was randomly divided into 4 subgroups: model control group (n = 8), model massage group 1 (n = 8), model massage group 2 (n = 8). Model massage group (3 groups). Model preparation: 3-day- postnatalP3 after birth in young rats (P _ (3)) (P _ (3), P _ (3), P _ (3), P _ (3)). Unilateral common carotid artery ligation combined with hypoxia-ischemia was used to establish the animal model. Spinal massage was performed at 1 day after establishment of the model. The body weight and eye opening time of young rats were measured at the same time point, and the tilting plate experiment was carried out. Results compared with model control group, the weight of blank control group was higher than that of model control group. The time of eye opening and the results of tilting plate experiment were significantly different, which showed that the model was successful. Compared with the model control group, model massage 1 and model massage 2. The body weight of the model massage group increased significantly (P 0.05), but there was no significant difference between the two groups (P 0.05). Eye opening time: model massage group 1 model massage group 2, model massage group 3, except model massage group 2 and model massage group 3, there was no significant difference between model massage group 2 and model massage group 3 (P < 0. 285). The angle of tilting plate: model massage, model massage, model massage, model massage, model massage, model massage, model massage, model massage, model massage, model massage, model massage, model massage, model massage, model massage, model massage, model massage, model massage, model massage, model massage, model massage. Conclusion Spinal massage can promote the growth and development of hypoxia-ischemic cerebral palsy and the recovery of motor function in the young rats with hypoxia-ischemic cerebral palsy. The length of massage is not proportional to the effect.
【作者单位】：云南中医学院;
【基金】：国家自然科学基金(No.81460748)
【分类号】：R244.1
【正文快照】： 的发病率约为1.92‰[2]。CP作为一种难以治愈的疾病,不仅对患者的家庭带来了沉重的经济负担,也影响患者家属的心理健康[3]。Guzzetta等[4]发现推拿可促进正常幼鼠大脑发育的成熟。Tai等[5]的研究结果显示,新生大鼠缺氧缺血后,其体质量增长、睁眼时间及切牙萌出皆表现迟缓。小

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