川芎平喘合剂对慢性阻塞性肺疾病肺动脉高压大鼠肺动脉平滑肌细胞增殖及对Rho激酶的影响

发布时间：2018-01-16 20:22

本文关键词：川芎平喘合剂对慢性阻塞性肺疾病肺动脉高压大鼠肺动脉平滑肌细胞增殖及对Rho激酶的影响　出处：《辽宁中医杂志》2017年12期 　论文类型：期刊论文

【摘要】：目的:观察川芎平喘合剂对肺动脉平滑肌细胞(PASMCs)增殖及对Rho激酶的影响。方法:体外培养PASMCs,分为对照组、模型组、川芎平喘合剂组(低、中、高浓度组)、Rho激酶抑制剂组。采用CCK-8方法测定PASMCs增殖,Western blot测定PASMCs中ROCK1、ROCK2表达水平。结果:与正常对照组相比,模型组PASMCs增殖明显升高,ROCK1、ROCK2表达增强;Rho激酶抑制剂组PASMCs增殖、ROCK1、ROCK2表达均显著低于模型组;与模型组相比,中、高剂量组PASMCs增殖水平显著降低,低、中、高剂量组ROCK1、ROCK2表达低于模型组,以高剂量组降低显著;低、中剂量组ROCK1、ROCK2表达均低于抑制剂组(P0.05或P0.01)。结论:川芎平喘合剂可明显抑制COPD相关肺动脉高压大鼠PASMCs增殖,与降低Rho相关蛋白表达有关。
[Abstract]:Objective: to observe the effect of Chuanxiong Pingchuan mixture on proliferation of pulmonary artery smooth muscle cells (PASMCs) and on Rho kinase. Methods: PASMCs were cultured in vitro and divided into control group and model group. Rho kinase inhibitor group in low, middle and high concentration groups were treated with Chuanxiong Pingchuan mixture. PASMCs proliferation was measured by CCK-8 method. Results: compared with the normal control group, the PASMCs proliferation in the model group was significantly higher than that in the normal control group. The expression of ROCK1 was enhanced. The expression of PASMCs proliferative roCK1 roCK2 in Rho kinase inhibitor group was significantly lower than that in model group. Compared with the model group, the proliferative level of PASMCs in the high dose group was significantly lower than that in the model group, and the expression of ROCK1, ROCK2 in the high dose group was significantly lower than that in the model group, especially in the high dose group. ROCK1 in low and middle dose groups. The expression of ROCK2 was lower than that of the inhibitor group (P0.05 or P0.01). Conclusion: Chuanxiong Pingchuan mixture can significantly inhibit the proliferation of PASMCs in rats with COPD associated pulmonary hypertension. It is related to the reduction of Rho related protein expression.
【作者单位】：上海中医药大学附属龙华医院;
【基金】：国家青年自然科学基金(81302922)
【分类号】：R259;R-332
【正文快照】： 肺动脉高压是肺心病形成的关键环节,肺心病是最终导慢性阻塞性肺疾病(chronic obstructive pulmona-ry diseases,COPD)相关死亡的主要原因之一,COPD病程逐渐延长及病势不断加重,肺动脉高压(pulmona-ry arterial hypertension,PAH)发生率也逐渐升高,p H为COPD病程中的单独危险

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