电针百会、神庭对脑缺血再灌注损伤大鼠学习记忆能力及突触可塑性的影响

发布时间：2018-01-16 20:43

本文关键词：电针百会、神庭对脑缺血再灌注损伤大鼠学习记忆能力及突触可塑性的影响　出处：《康复学报》2017年01期 　论文类型：期刊论文

【摘要】：目的:探讨电针百会、神庭穴对脑缺血再灌注损伤大鼠学习记忆能力及突触可塑性的影响。方法:将36只清洁级雄性SD大鼠随机分为假手术组、模型组和电针组,其中12只仅分离血管作为假手术组,其余24只运用线栓法制备左侧大脑中动脉局灶性脑缺血再灌注大鼠模型,缺血时间为2 h。造模成功后采用随机数字表法分为模型组和电针组各12只。电针组大鼠给予电针百会、神庭穴,1次/d,持续7 d;其余2组同等条件抓取,不予干预。各组大鼠在造模成功后第3天开始采用Morris水迷宫检测大鼠学习记忆能力,水迷宫试验共持续5 d;采用透射电镜观察海马区突触超微结构的变化;免疫组化法检测突触后致密蛋白95(PSD-95)和突触囊泡膜蛋白-突触素(SYN)的表达。结果:水迷宫测试中,模型组与假手术组相比逃避潜伏期明显增加(P0.001),穿越平台次数减少(P0.01);电针组与模型组比较逃避潜伏期缩短(P0.01),穿越平台次数增加(P0.05),第5天的穿越平台轨迹较集中于平台附近,而模型组则较分散和不规则。通过透射电镜观察,模型组与假手术组相比突触数量显著减少(P0.001);电针组比模型组海马CA1区突触的结构更加清晰和完整,数量显著增加(P0.01)。免疫组化发现假手术组比模型组海马区PSD-95及SYN表达显著增高(P0.001);与模型组相比,电针组海马区的PSD-95表达明显增高(P0.001),SYN表达也显著增高(P0.01)。结论:电针百会、神庭穴可以改善脑缺血再灌注损伤大鼠学习记忆能力,其机制可能与增强突触可塑性,包括促进突触结构的完整,增加PSD-95和SYN的表达有关。
[Abstract]:Objective: to investigate the effects of electroacupuncture at Baihui and Shenting points on learning and memory ability and synaptic plasticity in rats with cerebral ischemia-reperfusion injury. Methods: Thirty-six clean male SD rats were randomly divided into sham operation group. In the model group and electroacupuncture group, 12 isolated blood vessels were used as sham-operation group, the remaining 24 rats were used to establish focal cerebral ischemia-reperfusion model of left middle cerebral artery. The ischemia time was 2 hours. After the model was established successfully, the rats were randomly divided into two groups: the model group (n = 12) and the electroacupuncture group (n = 12). The rats in the electroacupuncture group were given electroacupuncture at Baihui, Shenting acupoint once a day for 7 days; The other two groups were grabbing under the same conditions without intervention. The Morris water maze was used to test the learning and memory ability of the rats on the 3rd day after the model was made successfully. The water maze test lasted for 5 days. The ultrastructure of synapses in hippocampal area was observed by transmission electron microscope (TEM). The expression of postsynaptic dense protein 95 (PSD-95) and synaptic vesicle protein-synaptophysin (SYN) were detected by immunohistochemical method. Compared with the sham operation group, the escape latency in the model group was significantly increased (P 0.001), and the frequency of crossing the platform was decreased (P 0.01). Compared with the model group, the electroacupuncture group shortened the escape latency (P0.01A), increased the frequency of crossing the platform (P0.05A), and the traversing track of the platform on the 5th day was more concentrated in the vicinity of the platform. The number of synapses in the model group was significantly lower than that in the sham-operated group (P 0.001). Compared with the model group, the synapse structure of the hippocampal CA1 area in the electroacupuncture group was clearer and more complete. The expression of PSD-95 and SYN in the hippocampus of the sham operation group was significantly higher than that of the model group. Compared with the model group, the expression of PSD-95 in the hippocampal area of the electroacupuncture group was significantly higher than that in the model group. Shenting acupoint can improve the learning and memory ability of rats with cerebral ischemia reperfusion injury, and its mechanism may be related to enhancing synaptic plasticity, including promoting the integrity of synaptic structure and increasing the expression of PSD-95 and SYN.
【作者单位】：福建中医药大学康复医学院;福建省康复产业研究院技术创新平台;福建省康复技术重点实验室;
【基金】：国家自然科学基金项目(81403462) 福建省教育厅资助省属高校项目(JK2014022)
【分类号】：R245
【正文快照】： 脑卒中后会引起一系列功能障碍,大约65%脑卒中患者会引起认知障碍,这已经严重地影响患者的日常生活[1-4]。学习记忆属于认知的一个重要方面,而海马区作为学习记忆的关键部位容易缺血缺氧[5],因此本实验选取海马为研究部位。课题组前期研究已证明电针百会、神庭穴能够有效改善

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