针灸与振动训练对缺血缺氧性脑病大鼠细胞炎症反应的研究

发布时间：2018-01-18 02:24

本文关键词：针灸与振动训练对缺血缺氧性脑病大鼠细胞炎症反应的研究　出处：《中华中医药学刊》2017年06期 　论文类型：期刊论文

【摘要】：目的:观察针灸与振动训练对于缺血缺氧性脑病鼠炎症反应的影响,初步探究针灸和振动训练治疗缺血缺氧性脑病的作用机制。方法:将成功建立缺血缺氧性脑病模型的60只SD大鼠,根据数字随机表法分为4组,即模型对照组(不接受治疗)、针刺组(仅接受针刺治疗)、训练组(仅接受振动训练)以及联合组(针刺联合振动训练)各15只,在建模前、建模后24h以及干预后2周时分别对大鼠神经功能进行评分,同时应用ELISA法检测血清IL-1、IL-6表达水平,应用RT-PCR检测脑组织中TNF-αmRNA、NF-κB mRNA表达水平,同时利用SPSS 19.0对实验结果进行统计分析。结果:建模后,各组大鼠的神经行为评分、血清IL-1、IL-6、脑组织TNF-αmRNA、NF-κB mRNA显著高于建模前(P0.05),但组间数据无明显差异(P0.05);干预后,联合组、针刺组、训练组神经行为评分、血清IL-1、IL-6、脑组织TNF-αmRNA、NF-κB mRNA均明显下降,且联合组各指标下降最显著,其次为针刺组,在干预2周时,各组上述指标差异显著(P0.05)。结论:针灸联合振动训练能够明显减轻缺血缺氧性脑病细胞炎症反应,其作用机制考虑与抑制IL-1、IL-6、TNF-α以及NF-κB等炎症因子表达有关。
[Abstract]:Objective: To observe the effect of acupuncture and vibration training for hypoxic ischemic encephalopathy rat inflammatory response, preliminary study the mechanism of acupuncture and moxibustion and vibration training in the treatment of hypoxic ischemic encephalopathy. Methods: 60 SD rats of hypoxic ischemic encephalopathy model was established successfully, according to figures were randomly divided into 4 groups: model control group (no treatment), acupuncture group (received acupuncture treatment), training group (received vibration training) and combined group (acupuncture combined with vibration training) 15 rats each, before modeling, modeling after 24h and 2 weeks respectively after the intervention of the rat nerve function score, and serum IL-1 by ELISA method, the expression level of IL-6 and TNF- in brain tissue was detected using RT-PCR mRNA NF- kappa B alpha, mRNA expression level, while using SPSS 19 on experimental results were analyzed. Results: after modeling, the rats neurobehavioral score, serum IL-1, IL-6, brain group The fabric TNF- alpha mRNA NF- kappa B mRNA were significantly higher than that before modeling (P0.05), but no significant differences between groups of data (P0.05); intervention group, acupuncture group, training group, neurobehavioral score, serum IL-1, IL-6, TNF- in brain tissue mRNA NF- kappa B alpha, mRNA were significantly decreased, and the joint the group index decreased significantly, followed by acupuncture intervention, in 2 weeks, each of these indicators significantly difference (P0.05). Conclusion: acupuncture combined with vibration training can obviously reduce the hypoxic ischemic encephalopathy of inflammatory reaction, the mechanism of IL-6 and suppression of IL-1, NF-, and other inflammatory factor TNF- alpha kappa B expression.

【作者单位】：辽宁中医药大学;
【基金】：辽宁省自然科学基金项目(2014020185)
【分类号】：R245
【正文快照】： 缺氧缺血性脑病(hypoxic ischemic encephalopathy,即HIE)作为新生儿脑损伤性疾病之一,能够导致新生儿出现急慢性神经功能损伤,是小儿致残的主要病因[1-2]。目前临床对于新生儿HIE的发病机制仍存有较多未解之谜,且截止目前临床尚无HIE的根治性治疗手段,因此,积极进行HIE相关性

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