基于“耳鼻喉科脾胃论”应用补中益气颗粒对脾气虚型变应性鼻炎大鼠鼻黏膜STAT6 mRNA的影响及临床疗效研究

发布时间：2018-01-18 12:32

本文关键词：基于“耳鼻喉科脾胃论”应用补中益气颗粒对脾气虚型变应性鼻炎大鼠鼻黏膜STAT6 mRNA的影响及临床疗效研究　出处：《安徽中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：1.目的探讨补中益气颗粒(吉灵参)对脾气虚型变应性鼻炎大鼠鼻黏膜STAT6m RNA的影响及临床疗效研究。2.方法动物实验:以卵清白蛋白0.03毫克抗原,佐剂氢氧化铝粉末30mg复制AR模型;以大黄腹泻法、疲劳法、控制饮食法等多因素复合伤脾的方法造成脾气虚模型;用荧光定量PCR法检测大鼠鼻黏膜中stat6 m RNA的蛋白含量。临床实验:选取2015年3月~10月安徽中医学院第一附属医院60例变应性鼻炎患者为研究对象,随机分为补中益气颗粒治疗组和依巴斯汀对照组,各30例,均停用相关药物,对照区别用药后两组的症状体征积分。3.结果动物实验:补中益气颗粒组与正常对照组、AR组及脾虚AR组相比较,差异有显著统计学意义(P0.01)。AR组与脾虚AR组较正常组stat6 m RNA蛋白表达明显增强,经治疗后蛋白表达减弱。临床实验:经三周的观察,补中益气颗粒治疗组的的显效率为56.7%,有效率为80%,无效率为20%;依巴斯汀对照组显效率为66.7%,有效率为83.3%,无效为16.7%。相比较而言,两组治疗均有明显疗效,两组疗效经卡方检验,P=0.739,P0.05,差异无统计学意义,享有可比性。4.结论补中益气颗粒能明显降低脾气虚型变应性鼻炎大鼠鼻黏膜的STAT6 m RNA的蛋白含量;补中益气颗粒治疗变应性鼻炎的有效在依巴斯汀;补中益气颗粒对变应性鼻炎的疗效确切,未发现明显不良反应,在治疗时间足够长的情况下可以长期使用,临床上值得推广。
[Abstract]:Objective to explore the Buzhong Yiqi granules (Jiling ginseng). Study on the effect of STAT6m RNA on the nasal mucosa of allergic rhinitis rats with deficiency of spleen qi. 2. Methods Animal experiment: ovalbumin 0.03 mg antigen was used. The AR model was made by 30 mg aluminum hydroxide powder. Spleen Qi deficiency model was induced by multiple factors such as rhubarb diarrhea method, fatigue method and diet control method. The protein content of stat6 m RNA in rat nasal mucosa was detected by fluorescence quantitative PCR. From March 2015 to October, 60 patients with allergic rhinitis were selected from the first affiliated Hospital of Anhui College of traditional Chinese Medicine. They were randomly divided into Buzhong Yiqi granule treatment group and Evastin control group with 30 cases in each group. Results: animal experiment: the Buzhong Yiqi granule group was compared with the normal control group and the spleen deficiency AR group. The expression of stat6 m RNA in AR group and spleen deficiency AR group was significantly higher than that in normal group. After treatment, the protein expression decreased. Clinical trial: after three weeks of observation, the effective rate of Buzhong Yiqi granule treatment group was 56.7, the effective rate was 80, and the non-effective rate was 20; The effective rate of Ebastine control group was 66.7%, the effective rate was 83.3%, and the ineffective rate was 16.7%. Compared with the control group, the two groups had obvious curative effect, and the two groups were tested by chi-square test. There was no significant difference between P0. 739 and P0. 05. Conclusion Buzhong Yiqi granule can significantly reduce the protein content of STAT6 m RNA in nasal mucosa of allergic rhinitis rats with deficiency of spleen qi. Buzhong Yiqi granule in the treatment of allergic rhinitis is effective in Ebastin; Buzhong Yiqi granule has definite curative effect on allergic rhinitis and no obvious adverse reaction. It can be used for a long period of time and is worth popularizing clinically.
【学位授予单位】：安徽中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R276.1

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