维药神香草聚酰胺树脂柱40%乙醇洗脱物的成分分析及其对哮喘小鼠炎症的改善作用

发布时间：2018-01-22 06:48

本文关键词： 维药神香草 %乙醇洗脱物支气管哮喘成分分析炎症因子小鼠　出处：《中国药房》2017年25期 　论文类型：期刊论文

【摘要】：目的:对神香草40%乙醇洗脱物进行成分分析,并考察其对哮喘小鼠炎症的改善作用。方法:收集聚酰胺树脂柱40%乙醇洗脱物,采用高效液相色谱-高分辨率质谱技术对神香草40%乙醇洗脱物成分进行分析。将72只小鼠随机分为空白组(生理盐水)、模型组(生理盐水)、地塞米松组(阳性对照,1.6 mg/kg)和神香草40%乙醇洗脱物高、中、低剂量组(200、100、50 mg/kg),每组12只。除正常组外,其余各组小鼠均于实验第0、14天ip 0.2 m L卵白蛋白(OVA)致敏;于实验第25~31天ig给药,每天1次;给药结束后,用2 mg/mL OVA连续滴鼻激发7 d。末次滴鼻激发24 h后,检测各组小鼠肺泡灌洗液(BALF)中肿瘤坏死因子α(TNF-α)、白细胞介素4(IL-4)和干扰素(IFN-γ)水平;观察肺组织病理变化。结果:共鉴定出11种化合物,相对百分含量为40.89%,主要成分有迷迭香酸、木犀草素7-O-α-L-吡喃鼠李糖(1→6)-β-D-吡喃葡萄糖苷、金丝桃苷等。与空白组比较,模型组小鼠BALF中TNF-α、IL-4水平升高,IFN-γ水平降低,IL-4/IFN-γ比值增大(P0.01);肺组织破坏严重,血管周围有炎症细胞浸润。与模型组比较,地塞米松组和神香草40%乙醇洗脱物高、中剂量组小鼠BALF中TNF-α、IL-4水平降低,IFN-γ水平升高,IL-4/IFN-γ比值减小(P0.01);肺组织病理变化改善。结论:本研究建立的分析方法能够有效地分析神香草40%乙醇洗脱物中的化学成分;神香草40%乙醇洗脱物可调节哮喘小鼠肺组织炎症因子的释放,减轻哮喘小鼠肺组织的炎症病变。
[Abstract]:Objective: to analyze the components of Shenxiang 40% ethanol elution and to investigate its effect on improving the inflammation of asthmatic mice. Methods: the polyamide resin column 40% ethanol elution was collected. High performance liquid chromatography-high resolution mass spectrometry (HPLC-MS) was used to analyze the components of Shenxiang 40% ethanol elution. 72 mice were randomly divided into blank group (normal saline group) and model group (normal saline group). The dexamethasone group (positive control group: 1.6 mg / kg) and Shenxiang 40% ethanol elution group had high, middle and low doses (200mg / kg). 12 mice in each group were sensitized by 0.2 mL ovalbumin on the 14th day of the experiment except the normal group. The drug was given orally on the 25th day of the experiment, once a day; At the end of administration, 2 mg/mL OVA was used continuously for 7 days and 24 h after the last nasal drip. The levels of TNF- 伪 TNF- 伪, IL-4 and IFN- 纬 in alveolar lavage fluid (BALF) of each group were measured. Results: a total of 11 compounds were identified with a relative content of 40.89. The main components were rosemary acid and luteolin 7-O- 伪 -L-rhamnose 1. 鈫扖ompared with the control group, the level of TNF- 伪 and IL-4 in BALF of the model group increased and the level of IFN- 纬 decreased. The ratio of IL-4 / IFN- 纬 increased (P 0.01). Compared with the model group, the dexamethasone group and Shenxiang 40% ethanol elution were higher than those in the model group, and TNF- 伪 in the BALF of the middle dose group was higher than that in the model group. The level of IL-4 decreased and the level of IFN- 纬 increased. The ratio of IL-4 / IFN- 纬 decreased (P 0.01). Conclusion: the analytical method established in this study can effectively analyze the chemical constituents in the ethanol elution of Shenxiang 40%. Shenxiang 40% ethanol elution could regulate the release of inflammatory factors in lung tissue of asthmatic mice and alleviate the inflammatory lesion of lung tissue in asthmatic mice.
【作者单位】：新疆维吾尔自治区药物研究所;中国医学科学院/北京协和医学院药物研究所/天然药物活性物质与功能国家重点实验室;
【基金】：国家自然科学基金资助项目(No.81560644) 新疆维吾尔自治区中医民族医药科技人才培养项目(No.2016-03-03)
【分类号】：R29
【正文快照】： 神香草为唇形科植物硬尖神香草(Hyssopuscuspida-tus Boriss.)的干燥地上部分,维吾尔名为“祖发奇尼”,是维吾尔族民间习用药材[1]。神香草药用全草,有很强的香味,主要含黄酮类、挥发油类、有机酸及其酯类等化学成分[2],具有镇咳祛痰、清热利湿、杀菌的作用,在维吾尔医学中有

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