静息能量代谢率用于评价糖皮质激素肾阴虚肾阳虚证模型的作用

发布时间：2018-01-23 00:16

本文关键词： 糖皮质激素肾阴虚肾阳虚动物模型能量代谢率　出处：《中国实验动物学报》2017年03期 　论文类型：期刊论文

【摘要】：目的观察静息能量代谢率(resting metabolic rate,RMR)在评价糖皮质激素肾阴虚及肾阳虚证模型中的作用。方法 BALB/c雄性小鼠分为对照组、模型组、金匮肾气丸组、知柏地黄丸组,后三组小鼠均给予含皮质酮的饮用水,对照组给予含1%乙醇的饮用水。采用封闭式流体压力呼吸计测定动物RMR。实验结束,处死小鼠,检测肾周脂肪、附睾脂肪、股四头肌、胫骨前肌重量指数;采用ELISA法检测血清激素水平;肝脏、肾脏组织HE染色观察;检测肝组织丙二醛(malondialdehyde,MDA)含量、琥珀酸脱氢酶(succinate dehydrogenase,SDH)活性、细胞色素c氧化酶(cytochrome-c-oxidase,COX)活性、ATP水平。结果与对照组比较,模型组能量代谢率在实验开始第2天即显著升高,第4天达最高(P0.01),之后逐渐降低,至第66天显著降低(P0.05)。皮质酮造模66 d显著降低小鼠血清甲状腺素(T4)含量,升高脂肪重量指数、降低肌肉重量指数、升高MDA水平、抑制SDH、COX活性及ATP水平。两补肾药均能降低动物死亡率,降低肝组织MDA含量,并且金匮肾气丸能升高模型组第4天及66天的能量代谢率(P0.05),能显著提高肝组织SDH活性、COX活性及ATP水平(P0.01),知柏地黄丸未观察到类似作用。结论静息能量代谢率对评价糖皮质激素肾阴虚/肾阳虚证模型具有一定作用。
[Abstract]:Objective to observe resting energy metabolism rate (metabolic rate). Methods BALB/c male mice were divided into control group, model group, Jingui Shenqi pills group and Zhibai Dihuang pill group. The latter three groups of mice were given drinking water containing corticosterone and the control group were given drinking water containing 1% ethanol. The closed fluid pressure breathing meter was used to determine the animal RMR.At the end of the experiment, the mice were killed and the perirenal fat was detected. Epididymal fat, quadriceps femoris, tibial anterior muscle weight index; Serum hormone levels were detected by ELISA method. Liver and kidney tissues were observed by HE staining. The content of malondialdehyde (MDA) and succinate dehydrogenase (SDH) activity in liver tissue were measured. The activity of cytochrome c oxidase (COXase) and ATP level were compared with those in control group. In the model group, the energy metabolism rate increased significantly on the second day of the experiment, reached the highest level on the 4th day, and then decreased gradually. On the 66th day, corticosterone significantly decreased serum thyroxine T4 content, increased fat weight index and decreased muscle weight index. The level of MDA was increased, the activity of ATP and the activity of ATP were inhibited. Both kidney tonifying drugs could decrease the mortality rate and decrease the content of MDA in liver tissue. Jingui Shenqi Pill could increase the energy metabolism rate of the model group on the 4th and 66th day, and could significantly increase the activity of SDH and the level of ATP (P0.01). Conclusion the resting energy metabolism rate has a certain effect on the evaluation of glucocorticoid kidney yin deficiency / kidney yang deficiency model.
【作者单位】：复旦大学药学院;复旦大学附属华山医院;复旦大学中西医结合研究院基础理论与应用研究所;
【基金】：国家自然科学基金项目(编号:81373791)
【分类号】：R228;R-332
【正文快照】： 3.复旦大学中西医结合研究院基础理论与应用研究所,上海200040)changes of the liver and kidney were examined by HE staining.Malondialdehyde(MDA)content,succinate dehydrogenase(SDH)activity,cytochrome c oxidase(COX)activity and ATP level were measured.Results

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