恐伤孕鼠对其仔鼠脑皮质神经元早期发育影响的实验研究

发布时间：2018-01-29 02:01

本文关键词： 恐伤肾先天肾虚脑发育左归丸　出处：《时珍国医国药》2017年09期 　论文类型：期刊论文

【摘要】：目的根据肾藏精主髓,髓充于脑,肾主脑发育理论,研究先天肾虚影响脑皮质神经元早期发育的情况及补肾法的作用。方法 SD大鼠(雌雄2∶1)合笼,制备孕鼠,分为模型组、空白组、补肾组。模型组、补肾组采用梅花针叩击孕鼠头部和模拟地震平台两种方法复合不定时恐吓刺激孕鼠整个孕期直至生仔,补肾组在造模过程中同时给予左归丸灌胃治疗,空白组自然喂养至生产;检测出生当日仔鼠脑湿重体质比;血清FT3、FT4含量;光镜及电镜观察脑皮质神经元结构。结果与空白组比较,模型组仔鼠脑湿重体质比下降(P0.01),血清FT3、FT4含量显著性升高(P0.01),光镜下脑皮质神经元细胞个数明显减少,脑神经元超微结构残缺,轮廓凹凸不平,核形不规整,核膜塌陷;补肾组仔鼠脑湿重体质比下降(P0.01),血清FT3、FT4含量显著性升高(P0.01),脑皮质神经元细胞个数增多,脑神经元超微结构接近正常。结论复合法恐吓刺激孕鼠可引起胎产数下降,仔鼠脑湿重体质比、血清FT3、FT4变化,脑皮质层神经元发育异常;中药补肾法治疗后,可有效改善先天肾虚仔鼠脑早期不良发育。
[Abstract]:Objective to study the influence of congenital kidney deficiency on the early development of cortical neurons and the effect of tonifying kidney method according to the theory of kidney accumulation essence and marrow filling in brain and kidney main brain. Methods SD rats (male and female 2: 1) combined cage. The pregnant rats were divided into three groups: model group, blank group, kidney tonifying group. The model group and kidney tonifying group used plum blossom needle percussion on the head of pregnant rats and simulated seismic platform. In the kidney tonifying group, the rats were treated with Zuo Gui Pill in the course of model making, while the blank group was fed naturally to production. The ratio of brain wet weight to body weight was measured on the day of birth. Serum FT _ 3N FT _ 4 content; Results compared with the blank group, the wet weight ratio of brain in the model group was decreased (P 0.01), and the content of serum FT 3 FT 4 was significantly higher than that in the control group (P 0.01). Under light microscope, the number of neurons in cerebral cortex decreased obviously, the ultrastructure of brain neurons was incomplete, the contour was uneven, the nucleus was irregular, and the nuclear membrane collapsed. In the kidney tonifying group, the ratio of brain wet weight to body weight was decreased (P 0.01), the serum FT3 FT 4 content was significantly increased (P 0.01), and the number of cerebral cortical neurons was increased. Conclusion the ultrastructure of brain neurons is close to normal. Conclusion compound intimidation can induce the decrease of fetal birth rate, the ratio of wet weight to weight of brain, the changes of serum FT _ 3 and FT _ 4, and the abnormal development of cortical neurons. The treatment of tonifying kidney with traditional Chinese medicine can effectively improve the early dysplasia of brain of congenital kidney deficiency infant.
【作者单位】：湖北中医药大学;
【基金】：湖北省教育厅科学技术研究项目(No.D20152006) 国家中医药管理局全国名老中医药专家传承工作室建设项目(No.[2014]20)
【分类号】：R22
【正文快照】： Zuoguiwan肾藏精、生髓、通脑,脑为髓之海,故肾脑密切相关。现阶段大量研究表明,母体孕期环境对胎儿脑发育及成年后认知、行为及性格产生重大影响[1,2]。对于“恐伤肾”对仔鼠的影响研究越来越深入,如代谢组学[3,4]、先天肾虚孕育理论[5]、动物实验[6]。基于我们前期恐吓导致

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