脾虚对糖尿病炎症状态和氧化应激影响的相关研究

发布时间：2018-01-29 10:40

本文关键词： 糖尿病脾虚氧化应激炎症　出处：《广州中医药大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的：此次试验起源于于益气健脾治疗糖尿病的观点,结合氧化应激和炎症的研究热点,选择糖尿病为研究病种,注重于体内的炎症状态和血清氧化还原体系,主要分析2型糖尿病(Diabetes mellitus type 2, T2DM)患者炎症和氧化应激以及胰腺外分泌功能的变化,探讨脾虚(Spleen deficiency)通过氧化应激和炎症方面对糖尿病的影响.方法：1、按照目前临床通用的糖尿病诊断标准划分糖尿病病例范围,按照相关脾虚证的辨证标准,在糖尿病患者内选取30例患者设定为糖尿病脾虚组,选择排除相关脾虚证症状的糖尿病患者30例作为糖尿病非脾虚组,健康体检中心选择20例作为正常组参考对照。2、收集上述人员血清,检测其血清胰型淀粉酶(Pancreatic amylase, P-AMY);α淀粉酶(Alpha-amylase preparation, α-AMY);超敏C反应蛋白(Highsensitivity C-reactive protein, hs-CRP);白细胞介素6(Interleukin 6, IL-6);肿瘤坏死因子α (Tumor necrosis factor-alpha, TNF-α);丙二醛(Malondialdhyde, MDA);蛋白质羰基含量(Protein carbonyls content, PCO).3、指标检测时,采用酶联免疫吸附测定法(ELISA)测定血清P-AMY, α-AMY含量；采用双抗体夹心法(ELISA)测定血清炎症因子hs-CRP、IL-6、TNF-α含量；采用硫代巴比妥酸(2-Thiobarbituric acid, TBA)法测定血清MDA水平;采用2,4二硝基苯肼(DNPH)比色法测定血清PCO含量.结果：糖尿病脾虚患者的P-AMY含量不明显低于糖尿病非脾虚患者,高水平病例分布低于糖尿病非脾虚患者,正常组高水平病例分布多于糖尿病的两组,但平均水平对比无统计学意义;糖尿病脾虚患者的α-AMY含量不明显低于糖尿病非脾虚患者,高水平病例分布低于糖尿病非脾虚患者, 正常组高水平病例分布多于糖尿病的两组,但平均水平对比无统计学意义；糖尿病脾虚患者的炎症因子hs-CRP, TNF-α含量不明显高于糖尿病非脾虚患者,高水平病例分布高于糖尿病非脾虚患者,正常组高水平病例分布低于糖尿病的两组,但平均水平对比无统计学意义；糖尿病脾虚患者的炎症因子IL-6含量明显高于糖尿病非脾虚患者(P＜0.01),高水平病例分布高于糖尿病非脾虚患者,与正常组平均水平对比无统计学意义糖尿病脾虚患者的MDA, PCO含量高于糖尿病非脾虚患者,高水平病例分布高于糖尿病非脾虚患者,正常组高水平病例分布低于糖尿病的两组,但平均水平对比无统计学意义；结论：脾虚有降低胰腺外分泌功能的趋势,使血清胰型淀粉酶水平降低,脾虚有加重部分糖尿病患者体内炎症和氧化应激的趋势。
[Abstract]:Objective: this experiment originated from the point of view of invigorating qi and invigorating spleen to treat diabetes mellitus, combined with the research focus of oxidative stress and inflammation, select diabetes mellitus as the research disease, and focus on the inflammatory state and serum redox system in vivo. The changes of inflammation, oxidative stress and pancreatic exocrine function in patients with type 2 diabetes mellitus mellitus type 2 (T2DM) were analyzed. Objective: to investigate the effect of Spleen deficiency on diabetes mellitus by oxidative stress and inflammation. According to the current clinical general criteria for the diagnosis of diabetes mellitus cases divided into the scope, according to the relevant spleen deficiency syndrome differentiation criteria, 30 cases of diabetes patients were selected as the group of diabetes spleen deficiency. Thirty patients with diabetes without spleen deficiency syndrome were selected as non-spleen deficiency group, and 20 patients were selected as reference control group by the health examination center to collect the serum of the above mentioned persons. The serum levels of pancreatic amylase (Pancreatic amylase) and P-AMYYase were detected. 伪 -amylase preparation, 伪 -amylase, 伪 -amylase; High sensitivity C-reactive protein (hs-CRP); Interleukin 6, Interleukin 6, Interleukin 6; Tumor necrosis factor-alpha, TNF- 伪; Malondialdehyde (malondialdehyde); Protein carbonyls content, PCOC. 3, index detection. The contents of P-AMY, 伪 -AMY in serum were determined by enzyme-linked immunosorbent assay (Elisa). The level of serum inflammatory factor hs-CRPnIL-6 TNF- 伪 was determined by double antibody sandwich method (ELISAA). The serum MDA level was determined by thiobarbituric acid (TBA) method. The content of serum PCO was determined by DNPH colorimetry. Results: the content of P-AMY in diabetic patients with spleen deficiency was not significantly lower than that in patients with non-spleen deficiency of diabetes mellitus. The distribution of high level cases was lower than that of non-spleen deficiency diabetic patients, and the distribution of high level cases in normal group was higher than that in the two groups of diabetes mellitus, but the average level was not statistically significant. The content of 伪 -AMY in patients with spleen deficiency in diabetes mellitus was not significantly lower than that in patients with non-spleen deficiency of diabetes mellitus, the distribution of high level cases was lower than that in patients with non-spleen deficiency of diabetes mellitus, and the distribution of high level cases in normal group was more than that in two groups of diabetes mellitus. But the average level contrast has no statistical significance; The levels of inflammatory cytokines hs-CRP and TNF- 伪 in patients with spleen deficiency were not significantly higher than those in patients with non-spleen deficiency of diabetes mellitus, and the distribution of high level cases was higher than that in patients with non-spleen deficiency of diabetes mellitus. The distribution of high level cases in normal group was lower than that in diabetic group, but the average level was not statistically significant. The content of inflammatory factor IL-6 in diabetic patients with spleen deficiency was significantly higher than that in patients with non-spleen deficiency of diabetes (P < 0.01), and the distribution of high level cases was higher than that of patients with non-spleen deficiency of diabetes mellitus. Compared with the normal group, the average level of MDAand PCO in the patients with spleen deficiency was higher than that in the patients with non-spleen deficiency, and the distribution of the high level cases was higher than that in the patients with non-spleen deficiency of diabetes mellitus. The distribution of high level cases in normal group was lower than that in diabetic group, but the average level was not statistically significant. Conclusion: spleen deficiency has the tendency of decreasing pancreatic exocrine function, reducing serum pancreatic amylase level and exacerbating inflammation and oxidative stress in some diabetic patients with spleen deficiency.
【学位授予单位】：广州中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R259

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