藏药绿萝花水提物对成纤维细胞线粒体抗氧化作用的研究

发布时间：2018-01-30 05:28

本文关键词： 绿萝花成纤维细胞线粒体膜电位活性氧　出处：《动物医学进展》2017年02期 　论文类型：期刊论文

【摘要】：为探讨不同浓度的绿萝花制剂对线粒体的抗氧化作用及毒理作用,以小鼠胚胎成纤维细胞及其线粒体为研究对象,在最大无毒作用浓度(TC_0)的基础上设计3个浓度药物组,在TC_0下设计6个浓度药物组,以成纤维细胞线粒体膜电位(MMP)和线粒体总活性氧含量(ROS)为检测指标,在4个不同作用时间点,研究绿萝花对成纤维细胞线粒体的影响。结果表明,绿萝花制剂对鼠胚成纤维细胞(MEF)的TC_0为0.25g/mL,但ROS已急剧上升,MMP下降;绿萝花浓度为0.03g/mL时,抗氧化能力最强,随着浓度的上升(0.03g/mL~0.25g/mL),线粒体抗氧化能力减弱或消失;随着给药浓度的升高,细胞凋亡现象出现趋势明显,浓度高于TC_0时,表现出毒性作用,浓度升高,毒性增大。绿萝花干膏制剂0.03g/mL(以生药含量计算)具有抵抗由H_2O_2引起的MEF线粒体MMP下降和ROS的升高的功能,表现出一定的抗氧化能力和阻滞细胞凋亡发生的作用;TC_0及之上浓度具有细胞毒性作用。
[Abstract]:In order to study the antioxidation and toxicological effects of different concentrations of Loxanthus officinalis preparation on mitochondria, mouse embryonic fibroblasts and mitochondria were studied. Three concentration groups were designed on the basis of maximum nontoxic concentration (TC0), and six concentration groups were designed under TC_0. The mitochondrial membrane potential (MMP) and mitochondrial total reactive oxygen species (Ros) in fibroblasts were measured at four different time points. The results showed that the TC_0 of the preparation was 0.25 g / mL, but the ROS had increased sharply. MMP decreased; With the concentration of 0.03g / mL, the antioxidant ability of the mitochondria decreased or disappeared with the concentration of 0.03g / mL mLN 0.25g / mL. With the increase of drug concentration, the phenomenon of apoptosis appeared obviously. When the concentration was higher than TC_0, it showed toxic effect and the concentration increased. The toxicity was increased. The 0.03g / mL (based on the content of crude drugs) of the dried cream of Rosa ussuriensis had the function of resisting the decrease of MEF mitochondrial MMP and the increase of ROS induced by H _ 2O _ 2. It showed the function of antioxidation and arrest of apoptosis. TC_0 and its concentration have cytotoxic effects.
【作者单位】：西南民族大学生命科学与技术学院;四川金科药业有限公司;
【基金】：“十二五”国家科技支撑计划项目(2011BAD34B02-4) 西南民族大学研究生创新型科研项目(CX2015SZ090)
【分类号】：R29
【正文快照】： 藏药绿萝花为天南星科喜林芋属植物绿萝花的干燥花蕾,为西藏特有的二级保护珍贵药材,民间习以泡水饮用,用以糖尿病、高血压、高血脂、冠心病及各种血管脉管炎症等的防治。目前关于绿萝花的研究报道较少,仅局限于几种化学成分提取工艺研究[1];药理研究表明绿萝花具有增强机体对

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