针刺“项七针”穴组对颈椎间盘退变大鼠颈椎间盘Delta样配体4及发状分裂相关增强子1表达的影响

发布时间：2018-02-02 00:13

本文关键词： 针刺项七针颈椎间盘退变 Delta样配体发状分裂相关增强子　出处：《针刺研究》2017年04期 　论文类型：期刊论文

【摘要】：目的:观察针刺"项七针"穴组对颈椎间盘退变大鼠颈椎间盘Delta样配体4(Dll 4)及发状分裂相关增强子1(Hes 1)表达的影响,探讨针刺"项七针"穴组延缓颈椎间盘退变的作用机制。方法:SD大鼠随机分成正常组、模型组及项七针穴组,每组11只。采用动静力失衡法建立颈椎间盘退变大鼠模型。项七针穴组针刺双侧"风池""天柱""完骨"和"风府",留针20min,每日1次,共治疗4周。采用斜板实验观察大鼠的斜板角度变化,HE染色观察颈椎间盘形态结构,应用实时荧光定量和Western blot法检测颈椎间盘组织中Dll 4及Hes 1mRNA和蛋白表达。结果:与正常组比较,模型组大鼠斜板角度明显降低(P0.05);与模型组比较,项七针穴组大鼠斜板角度显著升高(P0.05)。正常组大鼠颈椎间盘形态结构正常,纤维环排列规整;模型组大鼠颈椎间盘形态结构不规则,髓核细胞的数量相对正常组明显减少;项七针穴组大鼠颈椎间盘形态结构接近正常组。与正常组比较,模型组大鼠颈椎间盘组织Dll 4mRNA表达降低(P0.05),项七针穴组颈椎间盘组织Dll 4mRNA表达量高于模型组(P0.05)。与正常组相比,模型组大鼠颈椎间盘组织Hes 1mRNA表达显著升高(P0.05)。项七针穴组大鼠Dll 4和Hes 1蛋白表达量明显高于模型组(P0.05)。结论:针刺"项七针"穴组减缓颈椎间盘退变可能与其调控Dll 4、Hes 1mRNA和蛋白表达相关。
[Abstract]:Objective: to observe the effect of acupuncture on the expression of Delta like ligand 4 (Dll 4) and hair mitosis related enhancer 1 (Hes1) in cervical intervertebral disc degeneration rats. Objective: to explore the mechanism of delaying cervical disc degeneration in the "Xiang Qi Needle" group. Methods the rats were randomly divided into normal group, model group and seven acupuncture points group. There were 11 rats in each group. The model of cervical intervertebral disc degeneration was established by using the method of dynamic and dynamic imbalance. The acupuncture group of seven acupuncture points was used to acupuncture bilateral "Fengchi", "Tianzhu", "end bone" and "Fengfu" for 20 minutes, once a day. After 4 weeks of treatment, the angle change of inclined plate was observed by oblique plate experiment and the morphological structure of cervical intervertebral disc was observed by HE staining. The expression of Dll _ 4 and Hes _ 1 mRNA and protein in cervical intervertebral disc were detected by real-time fluorescence quantitative analysis and Western blot. Results: compared with normal group. In the model group, the angle of inclined plate decreased significantly (P 0.05). Compared with the model group, the angle of inclined plate in the Xiangqi acupuncture group was significantly higher than that in the control group (P 0.05). The morphology and structure of cervical intervertebral disc were normal and the fibrous rings were arranged regularly in the normal group. The number of nucleus pulposus cells in the model group was significantly lower than that in the normal group. Compared with the normal group, the expression of Dll 4 mRNA in the cervical intervertebral disc of the model group decreased (P 0.05). The expression of Dll _ 4 mRNA in the cervical intervertebral disc tissue of the Xiangqi Acupuncture group was higher than that of the model group (P 0.05), compared with the normal group. The expression of Hes 1 mRNA in the cervical intervertebral disc of the model group was significantly higher than that in the model group (P 0.05), and the expression of Dll 4 and Hes 1 protein in the seven acupuncture points group was significantly higher than that in the model group (2). Conclusion: it is possible that the acupuncture of "Shengqi Needle" group to slow down the degeneration of cervical intervertebral disc may be related to the regulation of Dll _ 4. The expression of Hes 1 mRNA was related to protein expression.
【作者单位】：山东中医药大学针灸推拿学院;山东中医药大学第二附属医院;
【基金】：山东省自然科学基金资助课题(No.ZR 2014HM 068) 山东省重点研发计划项目(No.2015GSF 119019)
【分类号】：R245
【正文快照】： 颈椎病是由于颈椎间盘退变引起的慢性退行性疾病,是临床常见病和多发病。我国颈椎病的发病率约为3.8%~17.6%,且有逐年上升的趋势[1]。采用针刺治疗颈椎病疗效突出[2]。研究显示针刺治疗颈椎病能够调节机体的血液循环[3]、神经电生理[4]、血管活性物质[5]、氧自由基代谢[6]、椎

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