艾灸“肺俞、膏肓俞”对BLMA5所致肺纤维化大鼠肺上皮细胞E-cad基因表达的实验研究

发布时间：2018-02-02 09:08

本文关键词： 艾灸肺纤维化 E-cad 肺俞膏肓俞　出处：《辽宁中医杂志》2017年08期 　论文类型：期刊论文

【摘要】：目的:探究艾灸"肺俞、膏肓俞"抑制肺纤维化进程的作用机制。方法 :将81只8周龄SPF级大鼠随机分9组:空白对照组;模型组(7 d组、11 d组、23 d组、35 d组);艾灸组(11 d组、23 d组、35 d组);阳性对照(泼尼松)组,每组9只。采用气管内一次性注入博来霉素复制肺纤维化模型。各组造模均于第二天进行治疗及干预。艾灸组交替艾灸双侧肺俞和膏育俞,3壮/穴,5 mg/壮,1次/d,10次/疗程。泼尼松组以泼尼松灌胃。空白组和所有模型组与艾灸组采取同样的抓取和固定,不给予任何治疗。共治疗3个疗程,疗程间休息2 d。各组在设定的时间窗口处死动物,采用定量PCR法检测E-cad基因的表达水平。结果:艾灸治疗组E-cad基因表达量比同期模型组高,尤以第35 d明显。艾灸35 d组(0.32±0.07)明显高于模型35 d组(0.10±0.04)(P0.01)。艾灸组和泼尼松组无明显差异,统计学无意义(P0.05)。结论:艾灸"肺俞、膏肓俞"可抑制肺纤维化的进程,其内在机制可能与升高肺上皮细胞E-cad基因表达有关。
[Abstract]:Objective: to explore the mechanism of moxibustion on the process of pulmonary fibrosis. Methods: 81 8-week-old SPF rats were randomly divided into 9 groups: blank control group; Model group 7 d group 11 d group 23 d group 35 d group; Moxibustion group, 11 d group, 23 d group, 35 d group; Positive control group (prednisone). Pulmonary fibrosis model was induced by intratracheal injection of bleomycin into the trachea of 9 rats in each group. The rats in each group were treated and intervened on the second day. The moxibustion group alternately moxibustion bilateral Feshu and ointment Yushu 3 Zhuang / acupoint. Prednisone group was given prednisone intragastrically. The blank group and all model groups were treated with the same grasping and fixation as moxibustion group without any treatment. The animals in each group were killed at the set time window. Results: the expression of E-cad gene in moxibustion treatment group was higher than that in model group. Especially on the 35th day, 0.32 卤0.07 in moxibustion group was significantly higher than that in model 35 day group (0.10 卤0.04). There was no significant difference between moxibustion group and prednisone group. Conclusion: moxibustion of "Feshu and Yanhuangshu" can inhibit the process of pulmonary fibrosis, and its internal mechanism may be related to the increase of E-cad gene expression in pulmonary epithelial cells.
【作者单位】：成都中医药大学基础医学院;
【基金】：国家自然科学基金(81373734)
【分类号】：R245.81
【正文快照】： 肺纤维化(pulmonary fibrosis,PF)是以弥漫性肺泡单位肺泡炎症和间质纤维化为主要病理特征,以肺部影像学改变及进行性限制性通气困难为临床表现,并最终以呼吸衰竭为结局的一类疾病。其病因复杂,业已发现除SARS以外至少150种病因与之相关[1]。西医以免疫抑制和激素治疗为主,

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