苦柏颗粒洗剂治疗肛肠病术后疼痛的实验研究

发布时间：2018-02-04 20:52

本文关键词： 苦柏颗粒洗剂肛肠病术后疼痛皮肤安全性　出处：《黑龙江省中医药科学院》2016年硕士论文　论文类型：学位论文

【摘要】：目的：通过研究苦柏颗粒洗剂在肛肠病术后肛门疼痛的疗效,并对苦柏颗粒洗剂的镇痛作用、作用原理和皮肤安全性进行研究,为在临床中进行推广提供科学依据。方法：采用随机对照的方法,观察苦柏颗粒洗剂治疗疼痛的疗效。将大鼠随机分成5组,分别为空白组、1：5000高锰酸钾溶液对照组、苦柏颗粒洗剂高、中、低剂量组。(1)大鼠足底热板法,造模成功后,在最后一次涂抹药物后的第30分钟、60分钟和90分钟,通过统计大鼠在热板仪上的舔足时间来比较各组药物的镇痛作用；(2)药物注射扭体法,造模后分别在各组大鼠腹腔注射0.7%冰醋酸溶液,记录下大鼠15分钟内扭体反应出现的次数,来比较药物的镇痛效果；(3)ELISA法,对组大鼠造肛肠病术后疼痛模型,除模型对照组,其余各组切口分别给予相应药物换药,每日2次,治疗3天后,目内眦取血,用ELISA法测定每组大鼠血清中IL-6和IL-10含量的改变；(4)皮肤安全性研究。结果：实验结果表明,苦柏颗粒洗剂高剂量组、中剂量能提高大鼠足底的痛感觉阈值(P0.01或P0.05),也可以明显抑制冰醋酸溶液所导致的大鼠扭体反应现象(P0.05)；苦柏颗粒高剂量组、中剂量(分别为24%和12%)能使肛肠病术后疼痛模型大鼠血清中的致痛物质白介素-6(IL-6)含量明显降低、镇痛物质白介素-10(IL-10)含量升高(P0.05或P0.01)；通过皮肤安全性实验研究结果可见,苦柏颗粒洗剂经皮给药,无红肿瘙痒等反应。结论：苦柏颗粒洗剂对肛肠病术后肛门疼痛有确切的效果,疗效强于对照组；苦柏颗粒洗剂局部给药,有镇痛作用,可以使血清中的IL-6降低、IL-10的含量升高；苦柏颗粒洗剂在皮肤局部给药,无任何不良反应,安全性较高。
[Abstract]:Objective: to study the curative effect of Kubai granule lotion on anal pain after anorectal disease, and to study the analgesic effect, action principle and skin safety of Kubai granule lotion. Methods: the therapeutic effect of Kubai granule lotion on pain was observed by a randomized control method. Rats were randomly divided into five groups: blank group. 1: 5000 potassium permanganate solution control group, Kubai granule lotion high, medium, low dose group. 1) the rat plantar hot plate method, after the success of the model, 30 minutes after the last application of the drug. At 60 and 90 minutes, the analgesic effects of each group were compared by counting the time of licking foot on the hot plate. (2) to compare the analgesic effect of drug analgesia, 0.7% glacial acetic acid solution was injected intraperitoneally into each group of rats after drug injection and writhing method was used to record the times of writhing reaction in 15 minutes. The model of postoperative pain of anorectal disease was established in rats of group A by Elisa. Except for model control group, the other groups were given the corresponding drugs to change medicine twice a day, after 3 days of treatment, the blood was taken from the canthus of the eye. The changes of IL-6 and IL-10 in serum of each group were determined by ELISA method. Results: the results showed that the moderate dose of Kubai granule lotion could increase the threshold of pain sensation in the plantar of rats (P0.01 or P0.05). It also inhibited the writhing reaction induced by acetic acid solution in rats (P 0.05). In the high dose group, the middle dose (24% and 12, respectively) could significantly decrease the content of the pain-causing substance interleukin-6 in the serum of the model rats with postoperative pain of anorectal disease. The content of the analgesic substance interleukin-10 (IL-10) was higher than that of the control group (P 0.05 or P 0.01). The results of skin safety test showed that Kubai granule lotion had no reaction such as skin administration without redness and pruritus. Conclusion: Kubai granule lotion has definite effect on anal pain after anorectal disease, and the curative effect is better than that in control group. Local administration of Kubai granule lotion has analgesic effect, which can reduce the content of IL-10 in serum and decrease the content of IL-10. Kubai granule lotion is given locally in the skin without any adverse reaction and is safe.
【学位授予单位】：黑龙江省中医药科学院
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R266

【参考文献】