针康法对慢性低灌注大鼠认知功能及OLGs影响的实验研究

发布时间：2018-02-09 23:26

本文关键词： 针康法慢性低灌注认知功能少突胶质细胞　出处：《黑龙江中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:通过观察针康法对慢性低灌注大鼠认知功能、脑白质组织染色情况、胼胝体部位OLGs表达的影响,拟阐明针康法改善慢性低灌注导致的认知功能障碍的可能机制。方法:30只健康Wistar雄性大鼠随机分为假手术组(Sha),模型组(Mod),康复组(Reh),针刺组(Acu)及针康组(A+R),每组6只。除假手术组外,其它各组均采用双侧颈总动脉永久结扎法(2-V0)制备慢性低灌注模型;假手术组仅给予分离双侧颈总动脉(common carotid artery,CCA),既不结扎又不剪断。经过4周的头穴丛刺结合康复训练后,Morris水迷宫检测大鼠的认知功能,HE染色观察大鼠脑白质组织的形态学变化,免疫组化法检测大鼠胼胝体部位少突胶质细胞(OLGs)阳性数目的表达。结果:1.学习记忆能力改变:慢性低灌注造模后,Morris水迷宫评测大鼠逃避潜伏期延长、原平台搜索次数减少,与Sha组相比差异有统计学意义(P0.05)。经过4周干预后,Reh、Acu及A+R三组逃避潜伏期缩短、原平台搜索次数增多,与Mod组相比差异有统计学意义(P0.05),且A+R组的Morris水迷宫评测成绩优于Reh、Acu组。2.HE染色观察:Sha组大鼠的脑白质无明显的病理学改变;Mod组大鼠脑白质出现不同程度的病理学改变:呈现皮层下、胼胝体、脑室旁白质疏松,纤维排列凌乱,胼胝体出现空泡样改变,皮质下可见较大的软化灶等;Reh、Acu及A+R三组大鼠脑白质病理学改变较轻,神经纤维排列紧密,胼胝体空泡面积较少,与Mod组相比差异明显,且A+R组胼胝体空泡面积明显减少,排列更加紧密规则。3.免疫组化检测结果:头穴丛刺治疗和跑台训练干预均能够升高慢性低灌注大鼠胼胝体组织中OLGs的表达,Reh、Acu及A+R组与Mod组相比,差异有统计学意义(P0.05)。A+R组OLGs的表达高于Reh组及Acu组,差异有统计学意义(P0.05)。结论:1.针康法能改善慢性低灌注大鼠的认知功能,且优于单纯针刺治疗与单纯康复治疗。2.针康法可以明显改善慢性低灌注大鼠脑白质的病理学改变。3.针康法可以增加慢性低灌注大鼠脑白质OLGs的表达。
[Abstract]:Objective: to observe the effects of acupuncture therapy on cognitive function, white matter staining and OLGs expression in corpus callosum of chronic hypoperfusion rats. Objective to elucidate the possible mechanism of acupuncture therapy in improving cognitive dysfunction induced by chronic hypoperfusion. Methods 30 healthy male Wistar rats were randomly divided into sham-operated group, model group, Rehor group, acupuncture group (Acua) and acupuncture group (6 rats in each group). Except for the sham-operation group, The chronic hypoperfusion model was established by permanent ligation of bilateral common carotid artery (2-V0) in all other groups. In the sham operation group, the common carotid artery of bilateral common carotid artery was isolated and neither ligated nor cut off. After 4 weeks of scalp acupuncture combined with rehabilitation training, Morris water maze was used to detect the cognitive function and HE staining of rat brain white matter tissue. Immunohistochemical method was used to detect the expression of oligodendrocyte oligodendrocytes (OLGs) in corpus callosum of rats. Results: 1. The ability of learning and memory: the latency of escape was prolonged and the search times of the original platform were decreased after Morris water maze was used to evaluate the rats' ability of learning and memory. After 4 weeks of intervention, the escape latency of the three groups was shortened, and the search times of the original platform increased, compared with that in the Sha group. Compared with Mod group, the difference was statistically significant (P 0.05), and the results of Morris water maze evaluation in group A and R were better than those in group A and R. 2. Observation of white matter in white matter of rats in group A and R with HE staining. There were no obvious pathological changes in the white matter of brain in rats in group A and R. There were pathological changes in the white matter of brain of rats in group A and R. Change: subcortical, In the corpus callosum, the white matter of the ventricle was loose, the fibers were disorganized, the corpus callosum appeared vacuolar changes, the subcortical soft foci were large, and the pathological changes of the white matter in the brain were mild and the nerve fibers were closely arranged in the three groups. The area of corpus callosum vacuole was less than that of Mod group, and the vacuolar area of corpus callosum was significantly decreased in AR group. The results of immunohistochemical examination showed that the expression of OLGs in the corpus callosum of rats with chronic hypoperfusion was increased by the treatment of scalp acupuncture and treadmill training. The expression of OLGs in the corpus callosum of rats with chronic hypoperfusion was higher than that in group A and R, as compared with that in group Mod. The expression of OLGs in P0.05A / R group was significantly higher than that in Reh group and Acu group, and the difference was statistically significant (P 0.05). Conclusion the acupuncture therapy can improve the cognitive function of rats with chronic hypoperfusion. Acupuncture therapy can significantly improve the pathological changes of the white matter of chronic hypoperfusion rats. Acupuncture Kang method can increase the expression of OLGs in the white matter of chronic hypoperfusion rats.
【学位授予单位】：黑龙江中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R245

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