蒙药忠伦阿汤对胶原诱导性模型大鼠OPG及RANKL影响的研究

发布时间：2018-02-17 03:29

本文关键词： 蒙药忠伦阿汤 CIA OPG RANKL　出处：《中华中医药学刊》2017年09期 　论文类型：期刊论文

【摘要】：目的:研究蒙药忠伦阿汤对胶原诱导性(CIA)关节炎大鼠血清及关节中OPG/RANKL的影响。方法:将CIA大鼠模型分为正常组、模型组、蒙药忠伦阿汤高、中、低剂量组及雷公藤多苷片组。实验过程中定期观察大鼠的一般活动情况。ELISA法检测大鼠血清中抗CII抗体及OPG和RANKL的含量。大鼠踝关节病理切片HE染色光镜观察滑膜和软骨组织病理学形态结构的变化,免疫组化SP法检测大鼠踝关节中OPG及RANKL的表达情况。结果:造模大鼠血清中抗CII抗体含量均高于正常组;正常组大鼠血清及踝关节中OPG呈高表达而RANKL呈低表达,模型组大鼠OPG呈低表达而RANKL呈高表达,二者有显著差异(P0.05),各治疗组药物均可下调RANKL的表达并升高OPG的表达,使OPG/RANKL比值增大。结论:蒙药忠伦阿汤能够一定程度增加血清及关节中OPG的表达而减少RANKL的表达,使OPG/RANKL的比值增大。蒙药忠伦阿汤可能是通过主要抑制RANKL的表达并辅助增强OPG的表达,使OPG/RANKL比例增大,OPG能够竞争性结合RANKL而阻断RANK与RANKL的结合,来实现缓解或延缓类风湿关节炎的病情发展。
[Abstract]:Objective: to study the effect of Mongolian medicine Zhongluana decoction on OPG/RANKL in serum and joint of rats with collagen-induced arthritis. Methods: the CIA rat model was divided into normal group, model group, Mongolian medicine Zhonglun decoction high and middle group. Low dose group and tripterygium wilfordii polyglycoside group. The general activity of rats was observed regularly during the experiment. Elisa was used to detect the contents of anti CII antibody and OPG and RANKL in rat serum. The synovium was observed by HE staining in the pathological sections of the ankle joint of rats. And changes in the histopathological morphology of cartilage, Immunohistochemical SP method was used to detect the expression of OPG and RANKL in the ankle joint of rats. Results: the level of anti CII antibody in serum of model rats was higher than that of normal group, the expression of OPG in serum and ankle joint of normal rats was high, and that of RANKL was low. In the model group, the expression of OPG was low and the expression of RANKL was high, there was significant difference between the two groups (P 0.05). All the drugs in the treatment group could down-regulate the expression of RANKL and increase the expression of OPG. Conclusion: the Mongolian medicine Zhonglun A decoction can increase the expression of OPG in serum and joint and decrease the expression of RANKL to some extent. The ratio of OPG/RANKL was increased, and the ratio of OPG/RANKL to RANKL could be blocked by inhibiting the expression of RANKL and increasing the expression of OPG, so that the ratio of OPG/RANKL could be competitively combined with RANKL, thus blocking the combination of RANK and RANKL. To relieve or delay the development of rheumatoid arthritis.
【作者单位】：内蒙古医科大学;内蒙古自治区人民医院蒙医科;
【基金】：国家自然科学基金项目(81360574)
【分类号】：R29;R-332

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