基于代谢组学研究热补针法治疗类风湿关节炎寒证家兔的作用机制

发布时间：2018-02-19 19:14

本文关键词： 热补针法类风湿关节炎代谢组学　出处：《针刺研究》2017年03期 　论文类型：期刊论文

【摘要】：目的:采用代谢组学技术探讨热补针法治疗类风湿关节炎(RA)寒证的特异性作用机制。方法:青紫蓝家兔随机分为正常组、模型组、平补平泻组、捻转补法组、热补针法组,每组8只。以卵蛋白诱导联合低温冷冻法建立RA寒证模型。各治疗组分别运用平补平泻法、捻转补法、热补针法于双侧"足三里"穴进行针刺干预,治疗30min,每日1次,共治疗7d。治疗后观察各组家兔的痛阈值以及关节表面温度,采用气相色谱-质谱(GC-MS)检测结合模式识别法分析家兔血清中内源性代谢物的变化。结果:模型组家兔痛阈、关节表面温度较正常组降低(P0.05),各针刺组干预后痛阈、关节表面温度较模型组升高(P0.05),热补针法组高于平补平泻组、捻转补法组(P0.05)。与正常组相比,模型组家兔血液代谢物中亮氨酸、缬氨酸、异亮氨酸、花生四烯酸的含量升高(P0.05),α-酮戊二酸、柠檬酸、琥珀酸、葡萄糖、纤维糖、棕榈酸、硬脂酸、乳糖、d-核糖、D-甘露糖的含量降低(P0.05)。与模型组比较,各针刺组血清中代谢物均出现不同程度的回调(P0.05),且热补针法组对α-酮戊二酸、柠檬酸、琥珀酸、葡萄糖、纤维糖、d-核糖、D-甘露糖的回调明显优于平补平泻组和捻转补法组(P0.05)。结论:热补针法治疗RA具有确切的疗效,可能与对三羧酸循环与糖代谢的相对特异性调控有关。
[Abstract]:Objective: to investigate the specific mechanism of heat tonifying acupuncture in the treatment of cold syndrome of rheumatoid arthritis (RA) by using metabonomics technique. Methods: rabbits with bluish blue were randomly divided into normal group, model group, Pingbu Pingxiao group, twirling reinforcing method group and heat reinforcing acupuncture group. Eight rats in each group were used to establish RA cold syndrome model by ovalbumin induction combined with hypothermia freezing method. Each treatment group was treated by acupuncture intervention at the bilateral "Zusanli" points, which were treated once a day for 30 mins. After 7 days of treatment, the pain threshold and joint surface temperature were observed, and the changes of endogenous metabolites in rabbit serum were analyzed by GC-MS and pattern recognition. Results: the pain threshold of the model group was determined by GC-MS. Compared with the normal group, the joint surface temperature decreased P0.05, the pain threshold of each acupuncture group was higher than that of the model group, the temperature of the joint surface was higher than that of the model group, the temperature of the hot acupuncture group was higher than that of the normal group, and that of the twirling tonifying group was higher than that of the normal group. Compared with the normal group, the Leucine in the blood metabolites of the model group was higher than that of the control group. The contents of valine, isoleucine, arachidonic acid, 伪 -ketoglutaric acid, citric acid, succinic acid, glucose, fibronectose, palmitic acid, stearic acid, galactose-ribonucleic acid, D-mannose were increased. The metabolites in serum of each acupuncture group showed different degrees of callback (P0.05A), and the heat supplementation group had effect on 伪 -ketoglutaric acid, citric acid, succinic acid, glucose, glucose, and 伪 -ketoglutaric acid, citric acid. The callback of D-ribose D-mannose was significantly better than that of P0.05 in the two groups. Conclusion: the therapeutic effect of heat supplement acupuncture on RA may be related to the relative specific regulation of tricarboxylic acid cycle and glucose metabolism.
【作者单位】：甘肃中医药大学针灸推拿学院;甘肃省中医院针灸科;兰州大学第二医院中医科;
【基金】：国家自然科学基金(No.81260558) 国家中医药管理局甘肃郑氏针法学术流派传承工作室项目(No.2305135901)
【分类号】：R245

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