针刺天枢穴对结肠炎肠上皮细胞NF-κB信号通路的影响

发布时间：2018-02-21 20:28

本文关键词： 结肠炎 DSS 天枢穴针刺疗法核因子-κB 信号通路　出处：《中华中医药杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的:研究针刺"天枢穴"对DSS诱导结肠炎(IBD)的治疗作用及其对核因子-κB(NF-κB)信号通路的影响。方法:将30只C57BL/6小鼠随机分为空白组、模型组和天枢穴组,模型组和天枢穴组分别给予3%DSS水溶液造模,天枢穴组每日给予20min针刺干预,连续7d。记录每日DAI评分,取材后测量结肠长度,HE染色观察结肠病理变化,ELISA法检测结肠组织白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平,Western blot法检测结肠组织中NF-κB、IκBα蛋白磷酸化水平,免疫荧光检测结肠组织中NF-κB表达水平。结果:天枢穴组每日DAI评分显著低于模型组(P0.01);结肠长度针刺组显著长于模型组(P0.05);结肠组织HE染色观察发现天枢穴组炎性损伤明显轻于模型组;结肠组织中IL-6、TNF-α含量天枢穴组显著低于模型组(P0.01),NF-κB、IκBα磷酸化水平亦明显低于模型组(P0.01)。结论:针刺天枢穴可以明显地改善DSS诱导IBD症状及结肠组织炎性反应损伤程度,可能是通过抑制NF-κB信号通路而起到了治疗作用,针刺天枢穴可以作为IBD一种有效的治疗手段。
[Abstract]:Objective: to study the therapeutic effect of "Tianshu acupoint" on DSS induced colitis and its effect on NF-魏 B signal pathway. Methods: 30 C57BL / 6 mice were randomly divided into three groups: blank group, model group and Tianshu point group. The model group and Tianshu point group were given 3 DSS aqueous solution models respectively, and Tianshu acupoint group were treated with acupuncture for 20 minutes daily for 7 days. Daily DAI scores were recorded. The pathological changes of colon were observed by HE staining. The levels of interleukin-6 and TNF- 伪 in colon tissue were detected by Elisa and the phosphorylation of NF- 魏 B I 魏 B 伪 protein in colonic tissue was detected by Western blot. Results: the daily DAI score of Tianshu acupoint group was significantly lower than that of model group, the colonic length acupuncture group was significantly longer than that of model group (P 0.05), and the HE staining of colon tissue showed inflammatory lesion in Tianshu acupoint group. The injury was significantly lighter than that in the model group. The content of IL-6 TNF- 伪 in colon tissue in Tianshu group was significantly lower than that in model group. The phosphorylation level of I 魏 B 伪 in NF- 魏 B was also significantly lower than that in model group. Conclusion: acupuncture at Tianshu point can obviously improve the symptoms of IBD induced by DSS and the degree of inflammatory reaction injury in colon tissue. Acupuncture at Tianshu point may be an effective treatment for IBD by inhibiting NF- 魏 B signaling pathway.
【作者单位】：广州中医药大学;
【分类号】：R245

【相似文献】