电针对促排卵大鼠子宫VEGF、VEGFR2表达的影响

发布时间：2018-02-22 03:34

本文关键词： 电针体外受精-胚胎移植 VEGF VEGFR　出处：《南京中医药大学学报》2017年06期 　论文类型：期刊论文

【摘要】：目的观察电针刺激对激素促排卵大鼠子宫组织中VEGF、VEGFR2表达的影响,并探讨其作用机制。方法 10周龄SD雌性大鼠30只,随机分为对照组、模型组及治疗组,每组各10只。模型组和治疗组腹腔注射孕马血清促性腺激素(PMSG)20U,48h后注射同等剂量的人绒毛膜促性腺激素(hCG),与种鼠合笼,次晨分笼造模,治疗组在与种鼠合笼前1周连续电针治疗7d(刺激强度1mA,疏密波2/15Hz,持续刺激15min)。合笼后第3.5天处死实验动物,留取子宫组织,观察形态改变;采用免疫组化方法检测VEGF、VEGFR2蛋白的表达;采用qPCR技术测定子宫组织中VEGF、VEGFR2mRNA的表达,采用2~(-△△Ct)方法计算。结果肉眼观察对照组子宫形态规整,表面红润光滑;模型组子宫形态肿大扭曲,表面色泽暗红充血;治疗组子宫形态略微肿胀,表面红润,无充血现象。免疫组化可见VEGF、VEGFR2蛋白在3组子宫内膜上均有表达,平均光密度没有明显差异,但表达分布部位有差异。模型组相比于对照组,在基质细胞和腺体上的表达分布较为广泛。VEGFR2在子宫的肌层组织中表达有明显差异:对照组几乎没有表达,模型组表达则明显增高(P0.05),治疗组比模型组表达有明显下降(P0.05)。模型组子宫内膜组织VEGF mRNA的表达是对照组的0.68倍,下降趋势明显;治疗组是对照组的0.88倍,有上升趋势。模型组子宫内膜组织VEGFR2mRNA的表达是对照组的0.46倍,下降趋势明显;治疗组是对照组的0.82倍,有比较明显的上升趋势。结论电针可以调整促排卵大鼠子宫组织中VEGF及其受体的表达,从而调整激素促排卵导致的子宫内膜种植窗口期的同步性。
[Abstract]:Objective to observe the effect of electroacupuncture on the expression of VEGFR2 in uterine tissues of ovulatory rats induced by hormone, and to explore its mechanism. Methods Thirty female SD rats aged 10 weeks were randomly divided into three groups: control group, model group and treatment group. The model group and treatment group were injected with the same dose of human chorionic gonadotropin (hCGG) for 48 h after intraperitoneal injection of serum gonadotropin PMSG (PMSG) 20 U, and the mice were caged together with the mice. The experimental animals in the treatment group were continuously treated with electroacupuncture for 7 days (stimulation intensity 1 Ma, density wave 2 / 15 Hz) for 7 days (stimulation intensity 1 Ma, density wave 2 / 15 Hz). The experimental animals were killed on the 3.5 day after cage closure, and the uterine tissues were retained to observe the morphological changes, and the expression of VEGFFR2 protein was detected by immunohistochemical method. The expression of VEGFN VEGFR2 mRNA in uterine tissue was measured by qPCR technique. Results the uterine morphology of the control group was regular, the surface was ruddy and smooth, the uterine shape of the model group was enlarged and distorted, and the surface color was dark red and congestive. In the treatment group, the uterine shape was slightly swollen, the surface was ruddy, and there was no hyperemia. The expression of VEGF VEGFR2 protein was observed in the endometrium of the three groups with no significant difference in average optical density. Compared with the control group, the expression of VEGFR2 in stromal cells and glands in the model group was significantly different in the myometrium of uterus: there was almost no expression in the control group. The expression of VEGF mRNA in the model group was 0.68 times higher than that in the control group, and in the treatment group was 0.88 times higher than that in the control group, while the expression of VEGF mRNA in the model group was 0.68 times higher than that in the control group, while that in the treatment group was 0.88 times higher than that in the control group. The expression of VEGFR2mRNA in endometrium of the model group was 0.46 times of that of the control group, and decreased obviously, and the expression of VEGFR2mRNA in the treatment group was 0.82 times of that in the control group. Conclusion electroacupuncture can regulate the expression of VEGF and its receptors in uterine tissues of ovulatory rats, thus regulating the synchronism of endometrium implantation window induced by hormone ovulation.
【作者单位】：南京中医药大学针药结合重点实验室;南京医科大学第一临床医学院;
【基金】：国家自然科学基金(81403481,81473767) 江苏省高校自然科学基金面上项目(14KJB360003)
【分类号】：R245

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